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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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ASC-CASP1 Octamer

Plasmid

#164032

Purpose

Bacterial expression vector encoding a 5GSS-linked ASC(CARD)-CASP1(CARD) construct to express an octamer

Depositor

Hao Wu

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

CASP1, ASC (CASP1, PYCARD Human)

Tags

His6-MBP-TEV

Expression

Bacterial

Mutation

W169G (ASC), G20K (CASP1)

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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SUMO_TwinStrep_Csx30

Plasmid

#197611

Purpose

For protein purification of Csx30

Depositor

Feng Zhang

Article

Strecker et al Science. 2022 Nov 25;378(6622):874-881.

Insert

SUMO_TwinStrep_Csx30

Expression

Bacterial

Available Since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pB18cmBFP1A5

Plasmid

#14035

Depositor

Patrick Daugherty

Article

Mena et al Nat Biotechnol. 2006 Dec . 24(12):1569-7

Insert

BFP1A5

Use

Yeast/bacteria optimized codons

Tags

6xHis

Expression

Bacterial

Available Since

Feb. 14, 2007

Availability

Academic Institutions and Nonprofits only

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SOCS4B-c002

Plasmid

#53669

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

SOCS4B

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
pET11a_PTK2b_Kinase

Plasmid

#224242

Purpose

Expression of human PTK2b Kinase domain in E. coli

Depositor

Andrew Mesecar

Insert

ptk2b_kinase (PTK2B Human)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7 promotor

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAN4037

Plasmid

#74703

Purpose

Output plasmid: PPhlF-PBetI-YFP

Depositor

Christopher Voigt

Article

Nielsen et al Science. 2016 Apr 1;352(6281):aac7341. d

Insert

PPhlF-PBetI-YFP

Expression

Bacterial

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pIA033

Plasmid

#200420

Purpose

thrC::(bsPamCherry1 erm2), for bsPAmCherry1 fusions. PALM integration vector includes codon optimized PAmCherry1 FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Type

Empty backbone

Tags

bsPAmCherry

Expression

Bacterial

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pIA034

Plasmid

#200421

Purpose

amyE::(bsdronPA cm), for bsdronPA fusions. PALM integration vector includes codon optimized dronPA FP for B. subtilis. MCS for fast and interchangeable cloning.

Depositor

Rut Carballido-López

Article

Altinoglu et al bioRxiv 2023

Type

Empty backbone

Tags

bsdronPA

Expression

Bacterial

Available Since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pEB2-DsRed-Express

Plasmid

#103995

Purpose

Plasmid encoding DsRed-Express

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

DsRed-Express

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (R2A, K5E, N6D, T21S,…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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