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33,902 results

Showing: 1961 - 1980 of 33902 results

  1. pGEX4T1 SHP1 WT

    Plasmid
    #8578
    Depositor
    Ben Neel
    Insert
    SHP1 (PTPN6 Human)
    Expression
    Bacterial
    Available since
    June 17, 2005
    Availability
    Academic Institutions and Nonprofits only

  2. prhaBAD-pA-Hia5

    Plasmid
    #222303
    Purpose
    Plasmid for rhamnose inducible expression and purification of protein A-Hia5-6XHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    pA-Hia5
    Tags
    6His and protein A
    Expression
    Bacterial
    Mutation
    codon optimized for bacterial expression
    Promoter
    rhaBAD
    Available since
    Oct. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    222303-plasmid-map-sequence-id-447746

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  4. SPYtag-GFPnanobody

    Plasmid
    #214837
    Purpose
    This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Publication
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    SPYtag-GFPnanobody
    Tags
    His-TEV-SUMO-
    Expression
    Bacterial
    Promoter
    T5
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    214837-plasmid-map-sequence-id-425310

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  5. SPYtag-Histag-Avidin

    Plasmid
    #214836
    Purpose
    This plasmid encodes a protein (SPYtag-Histag-Avidin) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.
    Depositor
    Hironori Funabiki
    Publication
    Arimura et al eLife. 2025 May 20;13:RP103486. doi: 10.
    Insert
    SPYtag-Histag-Avidin
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    214836-plasmid-map-sequence-id-423115

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  6. pKM512

    Plasmid
    #140192
    Purpose
    For curing of Orbit-integrated plasmids and associated drug marker
    Depositor
    Kenan Murphy
    Publication
    Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-
    Inserts
    zeocin resistance gene
    Bxb1gp47 (directionality factor)
    Expression
    Bacterial
    Mutation
    Cloned in gp47 directinality factor in place of R…
    Promoter
    EM7 and Ptet
    Available since
    Jan. 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    140192-plasmid-map-sequence-id-418051

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  12. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

    Plasmid
    #140082
    Purpose
    Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter
    Depositor
    Keith Joung
    Insert
    pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
    Use
    In vitro transcription (mrna synthesis)
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    140082-plasmid-map-sequence-id-292704

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  13. pcDNA3.1.Gαi3-LgB91

    Plasmid
    #134359
    Purpose
    Nanoluc complementation assay. Expression of Gαi3 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi3. Addition of the HA epitope at N terminus of Gαi3.
    Depositor
    Julien Hanson
    Publication
    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gαi3-LgB91 (GNAI3 Human)
    Tags
    HA
    Expression
    Bacterial and Mammalian
    Promoter
    T7
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134359-plasmid-map-sequence-id-306392

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  19. pSF4 TetCMV intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid
    #119945
    Purpose
    Expresses SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats
    Depositor
    Jeffrey Chao
    Publication
    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Renilla luciferase
    Tags
    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available since
    March 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    119945-plasmid-map-sequence-id-483465

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  20. pGM1192

    Plasmid
    #115683
    Purpose
    E. coli-Streptomyces shuttle vector for gene expression
    Depositor
    Günther Muth
    Insert
    mCherry, aac(3)IV, tsr
    Tags
    Strep-tagII on mCherry, codon optimized
    Expression
    Bacterial
    Mutation
    mCherry codon optimized for Streptomyces
    Promoter
    SF14*
    Available since
    Feb. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    115683-plasmid-map-sequence-id-237128

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Showing: 1961 - 1980 of 33902 results