30,260 results

1. pDx_mScarlet3-SYFP2

   Plasmid

   #189764

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet3-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH2B_mCherry_IRES_neo3

   Plasmid

   #21044

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pH2B
   

   Use

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIB184 (EM)

   Plasmid

   #90194

   Purpose

   E. coli - Streptococci shuttle plasmid for gene expression in streptococci with P23 promoter

   Depositor

   Indranil Biswas

   Article

   Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

   Type

   Empty backbone

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-6P1-Vinculin tail

   Plasmid

   #107154

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

   Insert

   Vinculin (VCL Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNP2157

   Plasmid

   #222732

   Purpose

   pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Helper (WT)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUltra-5HTP

   Plasmid

   #178042

   Purpose

   Express modified ScTrpRS/tRNA for genetic incorporation of 5HTP to TAG codon

   Depositor

   Han Xiao

   Article

   Chen et al Chem. 2020 Oct 8;6(10):2717-2727. doi: 1

   Inserts

   Modified ScTrpRS for 5HTP incoporation
   suppressor tRNA for 5HTP incorporation
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   T107C, P254T, C255A

   Promoter

   Available since

   Jan. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPomyces-Sth1Cas9

   Plasmid

   #129552

   Purpose

   Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

   Depositor

   Fong Tian Wong, Mingzi M. Zhang

   Article

   Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

   Insert

   Sth1Cas9
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT7C-DNAJA1

   Plasmid

   #177655

   Purpose

   Recombinant protein synthesis of human DNAJA1

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   DNAJA1 (DNAJA1 Human)
   

   Use

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX Cis1-SH2

    Plasmid

    #46417

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Cis1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 90-214

    Promoter

    Tac

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pBS mCD4

    Plasmid

    #14613

    Depositor

    Dan Littman

    Article

    Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

    Insert

    CD4 (Cd4 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDF-1b-RFC140ΔN555

    Plasmid

    #175043

    Purpose

    Overexpression of human (Rfc1) p140ΔN555 in E.coli

    Depositor

    Brian Kelch

    Article

    Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    Replication factor C subunit 1 (RFC1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pQTEV-LRPAP1

    Plasmid

    #31327

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    low density lipoprotein receptor-related protein associated (LRPAP1 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACYCT2

    Plasmid

    #45799

    Depositor

    Luc Ponchon

    Article

    Ponchon et al Nucleic Acids Res. 2013 Aug;41(15):e150.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PBAD-his6-prkA-pACYC184

    Plasmid

    #41041

    Depositor

    Ichiro Matsumura

    Article

    Parikh et al Protein Eng Des Sel. 2006 Mar;19(3):113-

    Insert

    prkA
    

    Use

    Tags

    6xHis and T7 epitope tag

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMTTH+bromo5

    Plasmid

    #157803

    Purpose

    Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

    Depositor

    Michael Rosen

    Article

    Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

    Insert

    bromo5
    

    Use

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    pTAC

    Available since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX Socs3-SH2

    Plasmid

    #46506

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs3 (SOCS3 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 22-142

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHH0103 IQGAP1 WW domain

    Plasmid

    #104279

    Purpose

    Bacterial expression of WW domain from IQGAP1

    Depositor

    Sachdev Sidhu, Marius Sudol

    Insert

    IQGAP1 WW domain (IQGAP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    codon optimized for expression in bacteria

    Promoter

    tac

    Available since

    Aug. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX Socs1-SH2

    Plasmid

    #46504

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Socs1 (SOCS1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 1-166

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEZYHb

    Plasmid

    #18669

    Purpose

    pKW32 converted Gateway Destination vector for expressing hemoglobin tagged fusion proteins in bacteria

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Use

    Tags

    hemoglobin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge