30,738 results

1. pET28a-cMSP26

   Plasmid

   #199769

   Purpose

   Expresses split-intein fused MSP26 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSP26
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   Aug. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-cMSPdH4-6

   Plasmid

   #199766

   Purpose

   Expresses split-intein fused MSPdH4-6 for circularization during expression (internal His-Tag)

   Depositor

   Franz Hagn

   Article

   Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

   Insert

   cMSPdH46
   

   Tags

   His6-Tag

   Expression

   Bacterial

   Mutation

   internal His-Tag

   Available Since

   July 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX4T-1-6AlanineHE1

   Plasmid

   #165098

   Purpose

   bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1

   Depositor

   Arthur Haas

   Insert

   Uba1 (UBA1 Human)
   

   Tags

   GST-6-alanine

   Expression

   Bacterial

   Mutation

   None

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRT498-OtUBD

   Plasmid

   #190089

   Purpose

   Expresses a single OtUBD with an TEV-cleavable N-terminal 6xHis-MBP tag as well as an engineered N-terminal cysteine in E. coli

   Depositor

   Mark Hochstrasser

   Article

   Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

   Insert

   OtUBD
   

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNH-TrxT

   Plasmid

   #26106

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Tags

   6xHis, TEV cleavage site, and TrxA

   Expression

   Bacterial

   Promoter

   T7-lacO (lactose/IPTG inducible)

   Available Since

   Dec. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCutamp

   Plasmid

   #140632

   Purpose

   Plasmid-curing in Escherichia coli by targeting the AmpR promoter

   Depositor

   Sheng Yang

   Article

   Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

   Insert

   SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pYTK-DN2

   Plasmid

   #180283

   Purpose

   Spacer cassette (TU3)

   Depositor

   Andreas Milias-Argeitis

   Article

   Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

   Insert

   ConL2 - Spacer - ConRE
   

   Expression

   Bacterial

   Available Since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pNCS YFP

   Plasmid

   #91759

   Purpose

   Bacterial expression of a fluorescent protein, Yellow Fluorescent Protein (YFP)

   Depositor

   Erik Rodriguez, Roger Tsien

   Article

   Cubitt et al Trends Biochem Sci. 1995 Nov;20(11):448-

   Insert

   Venus YFP (A206K)
   

   Tags

   Hexa-Histidine tag, Xpress epitope for detection …

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a SnoopTag-mEGFP-SpyTag

   Plasmid

   #72325

   Purpose

   Bacterial expression of monomeric enhanced green fluorescent protein (mEGFP) containing SnoopTag at the N-terminus and SpyTag at the C-terminus, for programmed polyprotein synthesis.

   Depositor

   Mark Howarth

   Article

   Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

   Insert

   pET28a SnoopTag-mEGFP-SpyTag
   

   Tags

   N-terminal His6 tag

   Expression

   Bacterial

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTXB3-Flag PLK1 T210D

    Plasmid

    #68272

    Purpose

    bacterial expression of Plk1 T210D mutant

    Depositor

    Stefano Ferrari

    Insert

    PLK1 (PLK1 Human)
    

    Tags

    Flag and Mxe intein/chitin binding domain

    Expression

    Bacterial

    Mutation

    T210D

    Promoter

    T7

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pQhsCDtRNA-

    Plasmid

    #86015

    Purpose

    Multi-gene expression system with human box C/D proteins with U14

    Depositor

    Hong Li

    Article

    Peng et al PLoS One. 2014 Jul 31;9(7):e103096. doi:

    Inserts

    U14 (SNORD14A Human)
    15.5K (SNU13 Human)
    fibrillarin (FBL Human)
    NOP56 (NOP56 Human)
    NOP58 (NOP58 Human)
    

    Use

    Co-expression; purification

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    amino acids 1-401, amino acids 1-411, and amino a…

    Available Since

    Jan. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTHSSe_46

    Plasmid

    #109244

    Purpose

    pSC101 var4 BbsI cloning vector

    Depositor

    Christopher Voigt

    Article

    Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PJ23105

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAGD236

    Plasmid

    #219497

    Purpose

    pCDF-Duet deriviative with sfGFP flanked by 2 BsaI sites downstream of the first T7 promoter and independent msr and RT downstream of the second T7 promoter

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    sfGFP, Eco1 msr, Eco1 RT
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRE-Tn7-166

    Plasmid

    #118578

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mCardinal
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBbE8k-RFP

    Plasmid

    #35270

    Depositor

    Jay Keasling

    Article

    Lee et al J Biol Eng. 2011 Sep 20;5:12.

    Insert

    RFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    BAD

    Available Since

    Feb. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSFDuet-sumo-h-cGAS

    Plasmid

    #127161

    Purpose

    expression of human cGAS protein

    Depositor

    Thomas Tuschl

    Article

    Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

    Insert

    MB21D1 (CGAS Human)
    

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    None

    Available Since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Expression

    Bacterial

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEX Su(fu) (CT#99)

    Plasmid

    #13856

    Depositor

    Cliff Tabin

    Article

    Pearse et al Dev Biol. 1999 Aug 15. 212(2):323-36.

    Insert

    Su(fu) (SUFU Chicken)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nco I fragment from full length; ~2kbp- ATG to st…

    Available Since

    Feb. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

20. pMRE-Tn7-161

    Plasmid

    #118573

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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