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pTriEx4-Rac1-2G

Plasmid

#66110

Purpose

Second generation Rac1 FRET biosensor

Depositor

Olivier Pertz

Article

Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

Insert

Second generation Rac1 FRET biosensor

Tags

His-Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pLIB-HIS-TEV-Cyclin-B

Plasmid

#177012

Purpose

Generate baculovirus for insect cell expression of Cyclin-B with N-terminal Polyhistidine-TEV

Depositor

Andrea Musacchio

Article

Huis In 't Veld et al Protein Sci. 2021 Nov 17. doi: 10.1002/p

Insert

Cyclin B1 (CCNB1 Human)

Tags

Polyhistidine-TEV

Expression

Insect

Mutation

codon-optimised for insect cell expression

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pR BirA

Plasmid

#113640

Purpose

Insect cell expression vector for BirA Biotin Ligase

Depositor

James Moon

Article

Moon et al Methods Mol Biol. 2018;1799:165-181. doi

Insert

BirA Biotin Ligase (birA )

Expression

Insect

Promoter

Metallothionein

Available Since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pUCDM

Plasmid

#110742

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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Tubp-Gal4 in pCaSpeR4

Plasmid

#17747

Depositor

Liqun Luo

Article

Lee et al Neuron. 1999 Mar . 22(3):451-61.

Insert

GAL4 (GAL4 Budding Yeast)

Tags

Tubulin 1alpha promoter and hsp70 PolyA

Expression

Insect

Available Since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pTriEx-NLyn-Venus-Zdk1

Plasmid

#81042

Purpose

mammalian expression of plasma-membrane anchored Zdk1

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

Zdk1

Tags

6x His tag, FLAG, NLyn, and mVenus

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pACEBac1-PARP2-Flag-His-WT

Plasmid

#111574

Purpose

Expresses full length PARP2 in Sf9 (insect) cells

Depositor

Thomas Muir

Article

Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

Insert

PARP2 (PARP2 Human)

Tags

C-terminal 6xHis and C-terminal Flag

Expression

Insect

Promoter

polyhedrin promoter

Available Since

July 18, 2018

Availability

Academic Institutions and Nonprofits only

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pNos-PE2-attB

Plasmid

#149549

Purpose

Expression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.

Depositor

Norbert Perrimon

Article

Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

PE2

Use

CRISPR

Expression

Insect

Promoter

nanos (nos)

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pSPL

Plasmid

#110743

Purpose

Plasmid for the insect cell expression system, donor for plasmid fusions, two promoters, Multiplication Module M, LoxP site

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Expression

Insect

Promoter

Polyhedrin and p10 promoters

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLib6.6

Plasmid

#176652

Purpose

Expression of sgRNA under D. melanogaster U6-3 _(CR31539) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Type

Empty backbone

Use

Crispr

Expression

Insect

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

Plasmid

#185449

Purpose

Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

Depositor

Ahmet Yildiz

Article

Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

Inserts

TRF2 (TERF2 Human)
RAP1 (TERF2IP Human)

Tags

MBP, YBBR, and ZZ

Expression

Insect

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFL-EGFP-3xFlag-DEST

Plasmid

#134903

Purpose

Baculovirus co-expression of GFP and gateway cloned protein with 3x-N-terminal Flag tag

Depositor

Andrew Deans

Article

Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

Type

Empty backbone

Tags

3x-Flag

Expression

Insect

Promoter

polyhedron

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

Plasmid

#206434

Purpose

Vasa 463-661 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 463-661 (vas Fly)

Tags

EGFP

Expression

Insect

Mutation

F504E

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry (FspAI) (HK49)

Plasmid

#206453

Purpose

Expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-PH-mCherry-Oskar 139-240 (eLOTUS domain) (HK73)

Plasmid

#206461

Purpose

Oskar 139-240 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Oskar 139-240 (osk Fly)

Tags

PH mcherry

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1-OST4-mCherry (EcoRV) (XH26)

Plasmid

#206475

Purpose

Expression in Schneider cells for imaging

Depositor

Mandy Jeske

Article

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Type

Empty backbone

Expression

Insect

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTriEx-RhoA FLARE.sc Biosensor WT

Plasmid

#12150

Depositor

Klaus Hahn

Article

Pertz et al Nature. 2006 Apr 20. 440(7087):1069-72.

Insert

RhoA Biosensor (RHOA Human)

Tags

6xHis

Expression

Bacterial, Insect, and Mamm…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFB1_hMSH6

Plasmid

#129424

Purpose

Expression human MSH6 protein using baculovirus

Depositor

Peggy Hsieh

Article

Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

Insert

human MSH6 (MSH6 Human)

Expression

Insect

Promoter

Polyhedrin promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Article

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available Since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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