4,931 results

1. OA-1045B

   Plasmid

   #125004

   Purpose

   Expresses gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. OA-1045D

   Plasmid

   #125006

   Purpose

   Expresses gRNAs targeting hedgehog and wingless

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   wg gRNA (wg Fly)
   

   Expression

   Insect

   Promoter

   u6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. OA-1045E

   Plasmid

   #125007

   Purpose

   Expresses tRNA-flanked gRNAs targeting hid, eve, and hedgehog

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OA-986C

   Plasmid

   #125000

   Purpose

   Expresses dCas9-VPR driven by the bottleneck promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   dCas9-VPR
   

   Use

   Piggyback

   Expression

   Insect

   Promoter

   bottleneck

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. OA-986D

   Plasmid

   #125001

   Purpose

   Expresses dCas9-VP64 driven by the Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   dCas9-VP64
   

   Use

   Piggyback

   Expression

   Insect

   Promoter

   ubiquitin

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. OA-986E

   Plasmid

   #125002

   Purpose

   Expresses dCas9-VP64 driven by the bottleneck promoter

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   dCas9-VP64
   

   Use

   Piggyback

   Expression

   Insect

   Promoter

   bottleneck

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. OA-1045A

   Plasmid

   #125003

   Purpose

   Expresses gRNAs targeting hid and eve

   Depositor

   Omar Akbari

   Article

   Buchman et al Nat Commun. 2021 Jun 2;12(1):3281. doi:

   Insert

   eve gRNA (eve Fly)
   

   Expression

   Insect

   Promoter

   U6:3

   Available Since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pUC57-attB2-SA(1)-CRY2-mCherry

   Plasmid

   #160440

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pUC57-attB2-SA(0)-CRY2-mCherry

   Plasmid

   #160439

   Purpose

   To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

   Depositor

   Nicholas Tolwinski

   Article

   Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

   Insert

   Cryptochrome 2 (CRY2 Mustard Weed)
   

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R979-X57-008: Hs.KRAS4b(1-169)

    Plasmid

    #159690

    Purpose

    Baculovirus protein expression of KRAS4b (1-169)

    Depositor

    Dominic Esposito

    Article

    Dharmaiah et al Sci Rep. 2019 Jul 19;9(1):10512. doi: 10

    Insert

    Hs.KRAS4b(1-169) (KRAS Human)
    

    Expression

    Insect

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R989-X30-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Plasmid

    #159557

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G13D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    G13D

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R989-X32-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

    Plasmid

    #159559

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61R (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    Q61R

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. R989-X28-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Plasmid

    #159555

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) G12D (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    G12D

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. R989-X31-636: His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

    Plasmid

    #159558

    Purpose

    Baculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L

    Depositor

    Dominic Esposito

    Article

    Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

    Insert

    His6-MBP-tev-G-Hs.KRAS4b(1-188) Q61L (KRAS Human)
    

    Tags

    His6-MBP-tev

    Expression

    Insect

    Mutation

    Q61L

    Available Since

    Sept. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Hy_pMT Flag-URH49 MUT

    Plasmid

    #110126

    Purpose

    Expresses Flag-tagged human URH49 (DDX39A) with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    URH49 (DDX39A Human)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 amino acid mutations (RSFS motif changed to KSL…

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Hy_pMT Flag-Hel25E MUT

    Plasmid

    #110122

    Purpose

    Expresses Flag-tagged D. melanogaster Hel25E with 4 mutations that impact circRNA nuclear export activity

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Hel25E (Hel25E Fly)
    

    Tags

    Flag

    Expression

    Insect

    Mutation

    4 mutations (KKLN motif changed to RSFS)

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    Sept. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. OA-1050I (notch)

    Plasmid

    #132421

    Purpose

    express arrays of gRNA targeting Notch under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    notch gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. OA-1050K (firefly luciferase)

    Plasmid

    #132422

    Purpose

    express arrays of gRNA targeting Firefly Luciferase under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    firefly Luciferase gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. OA-1050U (cinnabar)

    Plasmid

    #132423

    Purpose

    express arrays of gRNA targeting Cinnabar under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    cinnabar gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. OA-1050V (wingless)

    Plasmid

    #132424

    Purpose

    express arrays of gRNA targeting Wingless under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    wingless gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. OA-1050Z4 (yellow)

    Plasmid

    #132425

    Purpose

    express arrays of gRNA targeting Yellow under dU6-3 promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    yellow gRNA array
    

    Expression

    Insect

    Promoter

    U6-3

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. OA-1052B (Ubiq-Fluc_Ubiq-Rluc)

    Plasmid

    #132426

    Purpose

    Express Firefly Luciferase-eGFP and Renilla Luciferase under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    Ubiq promoter
    firefly luciferase
    T2A-eGFP
    Renilla Luciferase-SV40-P10
    reversed ubiq promoter
    

    Expression

    Insect

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. OA-1050R (Ubiq-dCasRx)

    Plasmid

    #132417

    Purpose

    Express dCasRx under Ubiquitin-63E promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Insert

    dCasRx
    

    Expression

    Insect

    Promoter

    Ubiq

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. OA-1050L (UASt-CasRx)

    Plasmid

    #132418

    Purpose

    Express CasRx under UASt promoter

    Depositor

    Omar Akbari

    Article

    Buchman et al CRISPR J. 2020 Jun;3(3):164-176. doi: 10

    Inserts

    CasRx
    UASt+hsp70
    

    Expression

    Insect

    Promoter

    UASt

    Available Since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. UAS-u(XXL)Cas9

    Plasmid

    #127387

    Purpose

    Expresses Cas9 at very low level

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    uORF(XXL)-Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pKM33

    Plasmid

    #154167

    Purpose

    Aedes aegypti Rel2 (long isoform) with C-terminal IκB domain deleted, expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Tags

    FLAG

    Expression

    Insect

    Mutation

    C-terminal IκB domain deleted

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pKM5

    Plasmid

    #154166

    Purpose

    Aedes aegypti Rel2 (long isoform) expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus.

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel2 (long isoform)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pKM28

    Plasmid

    #154165

    Purpose

    Aedes aegypti Rel1A expressed under the IE1 promoter and hr5 enhancer from Autographa californica nuclear polyhedris virus

    Depositor

    Kevin Maringer

    Article

    Russell et al PLoS Negl Trop Dis. 2021 Feb 16;15(2):e0

    Insert

    Rel1A
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    IE1 promoter and hr5 enhancer from Autographa cal…

    Available Since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Opie2-PuroR[dsxM]

    Plasmid

    #131616

    Purpose

    Expresses a functional PuroR only in males and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Inserts

    PuroR[dsxM] (dsx Fly)
    dsRed
    

    Expression

    Insect

    Promoter

    Opie2 and hr5Ie1

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Opie2-NeoR[TraF]

    Plasmid

    #131617

    Purpose

    Expresses a functional NeoR only in females and dsRed in both genders.

    Depositor

    Omar Akbari

    Article

    Kandul et al Nat Commun. 2020 Apr 30;11(1):2106. doi:

    Insert

    NeoR[traF]
    

    Expression

    Insect

    Promoter

    Opie2

    Available Since

    May 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pF3BGX-mTurquoise2

    Plasmid

    #138390

    Purpose

    RMCE vector for generating C-terminal mTurqouise2 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    mTurquoise2
    

    Expression

    Insect

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pKM634

    Plasmid

    #134219

    Purpose

    Baculovirus expression of CENP-HIKM

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP HIKM
    

    Expression

    Insect

    Available Since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

33. pDK580

    Plasmid

    #134205

    Purpose

    Baculovirus expresion of CENP-L and CENP-N

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    CENP-L and His-CENP-N
    

    Expression

    Insect

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pKM412

    Plasmid

    #134213

    Purpose

    Baculovirus expression of CENP-P and CENP-O

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    his-CENP-P + O
    

    Expression

    Insect

    Available Since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pKM672

    Plasmid

    #134221

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 1-509
    

    Expression

    Insect

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKM692

    Plasmid

    #134224

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 235-509
    

    Expression

    Insect

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pKM673

    Plasmid

    #134222

    Purpose

    Baculovirus expression of CENP-C

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

    Insert

    GST-CENP-C 510-end
    

    Expression

    Insect

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Myo 9 Avi+Flag

    Plasmid

    #134969

    Purpose

    Expression and purification of c.elegans Myo 9 (Hum 7) using baculovirus from pFastBac1

    Depositor

    Martin Bähler

    Article

    Liao et al J Biol Chem. 2010 Aug 6;285(32):24933-42

    Insert

    Myo 9 (hum-7 Nematode)
    

    Tags

    Avi and Flag

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Jan. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. U6d-T

    Plasmid

    #117212

    Purpose

    Expresses gRNA in Aedes aegypti

    Depositor

    Omar Akbari

    Article

    Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

    Insert

    u6d promoter-gRNA
    

    Use

    Synthetic Biology

    Expression

    Insect

    Promoter

    U6

    Available Since

    Jan. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pFB HTB_hMSH6_T1219D

    Plasmid

    #135466

    Purpose

    Expression human MSH6_T1219D mutant protein using baculovirus

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Insert

    human MSH6_T1219D (MSH6 Human)
    

    Tags

    His-tag

    Expression

    Insect

    Mutation

    changed threonine to aspartic acid

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge