4,958 results
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Plasmid#22030PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
Tags6X His and mCeruleanExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available SinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
PRExpress-Bxb1-hsp70pA
Plasmid#158391Purposeheat shock inducible Bxb1 integrase for drosophila melanogasterDepositorInsertBxb1
ExpressionInsectAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
IMPT-10316:GABR1
Plasmid#157850PurposeHA-TEV-VFT-TMD-CC-PreX-eGFP in pFastBac1 for Sf9 expressionDepositorAvailable SinceAug. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
DDB1
Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectPromoterpolyhedrinAvailable SinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCFD5-NS
Plasmid#149546PurposeExpression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.DepositorTypeEmpty backboneUseCRISPRExpressionInsectPromoterU6Available SinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKRG3-mU6-PUb-hSpCas9
Plasmid#162162PurposeExpresses hSpCas9 (Ae. aegypti PUb promoter) with cloning site for sgRNAs (Ae. aegypti U6 promoter)DepositorInsertshSpCas9
sgRNA
UseCRISPRTagsN/AExpressionInsectPromoterAe. aegypti PUb and Ae. aegypti U6Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPaint-T2A-Gal4-3xP3-RFP
Plasmid#127556PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4-SV40 and 3xP3-RFP visible eye marker.DepositorInsertGal4
UseCRISPRExpressionInsectAvailable SinceAug. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
p{CFD4-3xP3::DsRed}
Plasmid#86864PurposeExpresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.DepositorTypeEmpty backboneExpressionInsectAvailable SinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIZ-EGFP-MYO10-HMM
Plasmid#87257PurposeExpression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).DepositorInsertMYO10 (Myo10 Mouse)
ExpressionInsectMutationMyo10 is truncated to contain aa.1-941PromoterOpIE2Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1-SNAPf
Plasmid#133073PurposePlasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tagDepositorAvailable SinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
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ORCO-T2A-QF-DBD
Plasmid#162525PurposePlasmid for CRISPR-mediated knock-in line that expresses the DNA-binding domain of QF by knocking-in a T2A-QF-DBD fragment replacing the stop codon of the endogenous Aedes aegypti Orco geneDepositorInsertOrco-left-HDR-arm; T2A-Zip-::QFDBD-SV40; 3xP3-dsRed-SV40; Orco-right-HDR-arm
ExpressionInsectAvailable SinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-His-Tipin
Plasmid#22891DepositorAvailable SinceJan. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-dALFANb(5mut)-mGreenLantern
Plasmid#219661PurposeGal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 5 destabilising mutations) fused to mGreenLanternDepositorInsertDestabilised ALFA nanobody (5 mutations) fused to mGreenLantern
ExpressionInsectAvailable SinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAW-Nb127D01-GFP
Plasmid#171571PurposeExpresses GFP-tagged anti-CXCR2 (human) recombinant llama nanobody (Nb127D01-GFP) in fly cellsDepositorAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGP-20XUAS-IVS-Syn21-jGCaMP8f-p10
Plasmid#162388PurposeGal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogasterDepositorInsertjGCaMP8f
Tags6xHisExpressionInsectMutationQ315LPromoterhsp70Available SinceMay 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-PUbECFP
Plasmid#47917PurposeDonor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.DepositorInsertECFP
TagsNLSExpressionInsectPromoterAedes aegypti polyubiquitin (PUb)Available SinceSept. 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
pE2n
Plasmid#17456Purposemodified pENTR vector for N-terminal 3x HA tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTags3xHAExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
p2.0VPI.EGFP
Plasmid#40868DepositorInsertmouse vasopressin promoter
UseAAVExpressionBacterial and InsectPromotermouse vasopressinAvailable SinceNov. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSL1180-HR-Aag2-loxP-PUb-RFP-loxP-3xFLAG-AGO1
Plasmid#162164PurposeSelectable homology donor template for Aag2 cell AGO1DepositorInsertsRFP
3xFLAG
UseCRISPR and Cre/Lox; Homology donor templateTagsAGO1 homology and loxPExpressionInsectPromoterAe. aegypti PUb and endogenous AGO1Available SinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits only