4,908 results

1. pTS26_QUAS_CsChrimson

   Plasmid

   #175548

   Purpose

   Expresses the optogenetic channel CsChrimson off of a QUAS promoter and contains Piggybac terminal repeats for transposon-mediated integration

   Depositor

   Leslie Vosshall

   Article

   Sorrells et al Elife. 2022 May 12;11:e76663. doi: 10.75

   Insert

   CsChrimson
   

   Use

   Tags

   tdTomato

   Expression

   Insect

   Mutation

   Promoter

   QUAS

   Available since

   May 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. PUC19_C_tag

   Plasmid

   #186659

   Purpose

   Empty donor plasmid for C-terminal tag of 3xFlag-3xHA.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   C-terminal 3xFlag-3xHA Tag
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. BacPAK Myc-S1

   Plasmid

   #19424

   Depositor

   Ronald Conaway, Joan Conaway

   Article

   Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

   Insert

   Rpn2 (PSMD1, RPN2 Human)
   

   Use

   Tags

   His and Myc

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 21, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pHACK-GAL4>QF2(newHA1)

   Plasmid

   #104873

   Purpose

   HACK Donor plasmids for converting GAL4 lines to QF2 lines using HACK method. Updated version of Addgene#80275 for easier cloning of inserts

   Depositor

   Christopher Potter

   Article

   Lin et al Genetics. 2016 Jun 22. pii: genetics.116

   Insert

   T2A-QF2
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBPHLWL-VP16AD

   Plasmid

   #26259

   Depositor

   Tom Clandinin

   Article

   Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

   Insert

   VP16AD-ZIP+
   

   Use

   Drosophila transgenesis

   Tags

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Feb. 2, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUAST-2x attB

   Plasmid

   #206362

   Purpose

   includes two standard UAS cassettes and a third cassette without a promoter

   Depositor

   Erdem Bangi

   Article

   Quintero et al Cell Rep. 2023 Nov 28;42(11):113370. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPING

   Plasmid

   #26046

   Depositor

   Ray Owens

   Article

   Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

   Insert

   SS peptide-LacZ-KHHHHHH
   

   Use

   Tags

   KHHHHHH and MGILPSPGMPALLSLVSLLSVLLMGCVAETG

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   May 17, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. Brp-T2A-QF2w-Hsp70-3XP3-dsRed

   Plasmid

   #141094

   Purpose

   Template plasmid for inserting QF2w into the Brp gene of Aedes aegypti with CRISPR/Cas9, to generate a pan-neuronal driver line.

   Depositor

   Carolyn McBride

   Article

   Zhao et al Cell Rep Methods. 2021 Jul 26;1(3):10004

   Insert

   bruchpilot
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   coding sequence downstream of CRISPR cut site, sy…

   Promoter

   Available since

   Sept. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. UAS-Syt::Halo7

   Plasmid

   #87648

   Purpose

   For creating drosophila transgenics expressing UAS-Syt::Halo7

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Syt::Halo7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   S265G in Syt (please see depositor's commen…

   Promoter

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBS-KS-attB1-2-PT-SA-SD-1-GFP11

    Plasmid

    #172068

    Purpose

    Protein trap plasmid for inserting GFP11 into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    GFP11x7
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pACU2

    Plasmid

    #31223

    Depositor

    Yuh-Nung Jan

    Article

    Han et al Proc Natl Acad Sci U S A. 2011 Jun 7;108

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFD2-dU6:2gRNA

    Plasmid

    #49409

    Purpose

    expression of gRNA under control of the Drosophila U6:2 promoter

    Depositor

    Simon Bullock

    Article

    Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

    Insert

    dU6-2:gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    dU6-2

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. 50 pLIB_STREP

    Plasmid

    #160275

    Purpose

    N-term 3C cleavable 2xStrepII tag for insect cell expression

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Use

    Tags

    2xStrepII

    Expression

    Insect

    Mutation

    Promoter

    polyhydrin

    Available since

    Dec. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJFRC206-10XUAS-FRT>STOP>FRT-myr::smGFP-V5

    Plasmid

    #63168

    Purpose

    GAL4-responsive UAS “flp-On” spaghetti monster GFP-V5 reporter

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

    Insert

    "spaghetti monster GFP-V5"
    

    Use

    Tags

    N-myristoylation

    Expression

    Insect

    Mutation

    Promoter

    GAL4-responsive UAS hsp70

    Available since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGP-20XUAS-IVS-Syn21-RSET-jGCaMP8m-p10

    Plasmid

    #218862

    Purpose

    Gal4 activated transgene expression of ultrafast protein calcium sensor under the hsp70 promoter in D. melanogaster

    Depositor

    GENIE Project

    Article

    Reagents et al figshare Dataset. 2024. doi: 10.6084/m9.

    Insert

    RSET-jGCaMP8m
    

    Use

    Tags

    6xHis-T7 gene 10 leader-Xpress epitope-Enterokina…

    Expression

    Insect

    Mutation

    A25G F286Y

    Promoter

    hsp70

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCoofy64

    Plasmid

    #122016

    Purpose

    Baculovirus vector for parallel SLIC cloning containing gp67 signal sequence, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Use

    Tags

    His6-Smt3Star and Multi3: depending on the LP2 pr…

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1 INI1

    Plasmid

    #1953

    Depositor

    Robert Kingston

    Article

    Phelan et al Mol Cell 1999 Feb;3(2):247-53.

    Insert

    INI1 (SMARCB1 Human)
    

    Use

    Tags

    flag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

18. pQF2wWB

    Plasmid

    #61313

    Purpose

    Enhancer trap version of QF2w, analogous to pGAWB

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    QF2w
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFBD-BirA_RACK1:1-317

    Plasmid

    #210889

    Purpose

    Insect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    RACK1:M1-R317 (RACK1 Human)
    

    Use

    Tags

    GGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG

    Expression

    Insect

    Mutation

    wild type

    Promoter

    Polyhedrin

    Available since

    March 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. OA-986F (AAEL003877-dCas9-VPR)

    Plasmid

    #183993

    Purpose

    Expresses dCas9-VPR under the Ae. aegypti polyubiquitin promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    AAEL003877-dCAS9-VPR
    

    Use

    CRISPR

    Tags

    dCas9-VPR

    Expression

    Insect

    Mutation

    Promoter

    AAEL003887

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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