4,908 results

1. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104619

   Purpose

   Expresses HA tagged Citrine or FLAG tagged Cerulean upon binding of TALE1 to VAS1 or TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4-hsp70-Syn21-FLAG-Cerulean-P10
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10

   Plasmid

   #104618

   Purpose

   Expresses FLAG tagged Cerulean upon binding of TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   hsp70-Syn21-FLAG-mCerulean-P10
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJFRC81-3xVAS4-IVS-Syn21-GFP-P10

   Plasmid

   #104615

   Purpose

   Expresses GFP upon binding of TALE4 to VAS4

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   3xVAS4
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJFRC81-3xVAS1-Syn21-Citrine-HA-P10

   Plasmid

   #104616

   Purpose

   Expresses HA tagged Citrine upon binding of TALE1 to VAS1

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Insert

   Syn21-Citrine-HA-P10-MhcSD
   

   Use

   Tags

   HA

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJC-20xUAS-TALE-VP64-P10

   Plasmid

   #104605

   Purpose

   Control plasmid (no TALE array) with P10 terminator

   Depositor

   Tudor Fulga

   Article

   Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

   Type

   Empty backbone

   Use

   TALEN

   Tags

   Expression

   Insect

   Mutation

   Promoter

   hsp70

   Available since

   Jan. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAFMW-AthHEN1-CM

   Plasmid

   #104981

   Purpose

   Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUAST-Myc-mSesn2 (1186)

   Plasmid

   #66920

   Purpose

   expression of mouse Sesn2 in drosophila

   Depositor

   Michael Karin, Jun Hee Lee

   Article

   Lee et al Science. 2010 Mar 5;327(5970):1223-8. do

   Insert

   Sesn2 (Sesn2 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Insect

   Mutation

   Promoter

   HS promoter

   Available since

   Dec. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMCh-623

   Plasmid

   #92266

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 6W6A with six mutated W-motifs in its N-terminal domain in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   dGW182 6W6A (gw Fly)
   

   Use

   Tags

   NHA

   Expression

   Insect

   Mutation

   The following point mutations were introduced W23…

   Promoter

   Ac5

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMCh-695

   Plasmid

   #92263

   Purpose

   Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 with mutated PAM2 motif in D. melanogaster cells

   Depositor

   Marina Chekulaeva, Witold Filipowicz

   Article

   Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

   Insert

   dGW182 PAM2 mut (gw Fly)
   

   Use

   Tags

   NHA

   Expression

   Insect

   Mutation

   The following point mutations were introduced: EF…

   Promoter

   Ac5

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMCh-61

    Plasmid

    #92262

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMCh-76

    Plasmid

    #92288

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 CED (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215

    Promoter

    Ac5

    Available since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMCh-646

    Plasmid

    #92289

    Purpose

    Plasmid for expression of NHA-tagged Drosophila melanogaster GW182 CED 7W7A with seven mutated W-motifs in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Witold Filipowicz

    Article

    Chekulaeva et al Nat Struct Mol Biol. 2011 Oct 7;18(11):1

    Insert

    dGW182 CED 7W7A (gw Fly)
    

    Use

    Tags

    NHA

    Expression

    Insect

    Mutation

    dGW182 CED: aa 940-1215 with the following point …

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMCh-59

    Plasmid

    #92261

    Purpose

    Plasmid for expression of HA-tagged Drosophila melanogaster GW182 CED in D. melanogaster cells

    Depositor

    Marina Chekulaeva, Roy Parker

    Article

    Chekulaeva et al RNA. 2009 May;15(5):794-803. doi: 10.126

    Insert

    dGW182 (gw Fly)
    

    Use

    Tags

    HA

    Expression

    Insect

    Mutation

    aa 940-1215

    Promoter

    Ac5

    Available since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    p10 and pH

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc-GFP-dSUMOdeltaC

    Plasmid

    #85697

    Purpose

    expression of GFP-SUMO1 delta C in Drosophila cells

    Depositor

    Licio Collavin, Giannino Del Sal

    Article

    Mauri et al J Biol Chem. 2008 Jul 25. 283(30):20848-

    Insert

    SUMO (Sumo Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    non-conjugatable SUMO, lacking the C-terminal gly…

    Promoter

    pAc5

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pFBD-Btk(PH)Akt1(KD)

    Plasmid

    #86591

    Purpose

    Expresses chimeric fusion of PH domain of Btk and kinase domain of Akt1

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    PH(Btk)-KD(Akt1) fusion (AKT1, BTK Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFBD-Akt13C(WT)

    Plasmid

    #86576

    Purpose

    Expresses cleavable human full-length Akt1(WT)

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    3C cleavage site inserted at pos. 130

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR221-OSF-TRIM5-21R(1-300)

    Plasmid

    #79029

    Purpose

    Gateway entry vector for cloning and propagation in DH5alpha

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    TRIM5-21R (1-300)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    T7

    Available since

    March 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFastBac1-OSF-TRIMCyp (owl monkey)

    Plasmid

    #79035

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5Cyp (owl monkey)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFastBac1-OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)

    Plasmid

    #79030

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(African green monkey pygerythrus)(1-293)
    

    Use

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Mutation

    deleted amino acid 294-515 from frican green monk…

    Promoter

    polyhedrin

    Available since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge