4,908 results

1. pB[U6-3 wex2 PUb-DsRed]

   Plasmid

   #169010

   Purpose

   For germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.

   Depositor

   Max Scott

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   white ex2 sgRNA
   

   Use

   Piggybac

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Drosophila melanogaster U6:3

   Available since

   April 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFL-EGFP-3xFlag-HLTF

   Plasmid

   #193055

   Purpose

   Baculovirus expression of human HLTF for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   HLTF (HLTF Human)
   

   Use

   Tags

   3xFlag

   Expression

   Insect

   Mutation

   Promoter

   polyhedron

   Available since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. act5c-LbCas12a

   Plasmid

   #140620

   Purpose

   Ubiquitous expression of LbCas12a in Drosophila

   Depositor

   Michael Boutros

   Article

   Port et al Proc Natl Acad Sci U S A. 2020 Aug 25. p

   Insert

   LbCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJFRC203-10XUAS-FRT>STOP>FRT-myr::smGFP-cMyc

   Plasmid

   #63167

   Purpose

   GAL4-responsive UAS “flp-On” spaghetti monster GFP-cMyc reporter

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2015 May 11. p

   Insert

   "spaghetti monster GFP-cMyc"
   

   Use

   Tags

   N-myristoylation

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB1.HMBP.PrS.RBBP4

   Plasmid

   #125164

   Purpose

   Expresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

   Depositor

   Chen Davidovich

   Article

   Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

   Insert

   Histone-binding protein RBBP4 (RBBP4 Human)
   

   Use

   Tags

   His-MBP

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   April 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAaePUb::Cas9-2A-Neo

   Plasmid

   #176680

   Purpose

   Expression of Drosophila codon optimized spCas9 under Ae. aegypti Polyubiquitin promoter (AAEL003888)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
   

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Drosophila codon optimized

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR651

   Plasmid

   #65213

   Purpose

   Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-1 cap gene in Sf9 insect cells

   Depositor

   Robert Kotin

   Article

   Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

   Inserts

   modified AAV-2 rep78
   modified AAV-1 cap
   

   Use

   AAV

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10 and polyhedrin

   Available since

   June 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2

   Plasmid

   #185449

   Purpose

   Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site

   Depositor

   Ahmet Yildiz

   Article

   Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.

   Inserts

   TRF2 (TERF2 Human)
   RAP1 (TERF2IP Human)
   

   Use

   Tags

   MBP, YBBR, and ZZ

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOPINS3C

   Plasmid

   #41115

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pActP-dtrpA1

    Plasmid

    #64713

    Purpose

    inducing calcium influx in cultured Drosophila S2 cells

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    dtrpA1 (TrpA1 )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOCC120-MBP-DDX3X-monoGFP-His

    Plasmid

    #219985

    Purpose

    DDX3X(WT) protein purification

    Depositor

    Simon Alberti

    Insert

    DDX3X(WT) (DDX3X Human)
    

    Use

    Tags

    MBP and monoGFP and 6xHis

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. OA-1053A

    Plasmid

    #184006

    Purpose

    Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNAeve1 - U6c:sgRNAeve2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. p{CFD4-EYFP-3xP3::DsRed}

    Plasmid

    #86863

    Purpose

    Expresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.

    Depositor

    David Stern

    Article

    Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTriEx-mCherry-Zdk1-TIAM1 DH/PH

    Plasmid

    #81129

    Purpose

    mammalian expression of Zdk1 tagged Tiam1 Dh/PH

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    TIAM1 DH/PH (TIAM1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTriEx-mVenus-PA-Rac1

    Plasmid

    #22007

    Purpose

    Expression of photoactivatable Rac1

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1 (RAC1 Human, Avena sativa (oat))
    

    Use

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pUASTattB-dALFANb(3mut)-mGreenLantern

    Plasmid

    #219662

    Purpose

    Gal4-inducible expression of the destabilised anti-ALFAtag nanobody (including 3 destabilising mutations) fused to mGreenLantern

    Depositor

    Markus Affolter

    Article

    Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

    Insert

    Destabilised ALFA nanobody (3 mutations) fused to mGreenLantern
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFB-GST-IRP2

    Plasmid

    #226621

    Purpose

    Expresses human IRP2 in insect cells

    Depositor

    Ning Zheng

    Article

    Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

    Insert

    IRP2 (IREB2 Human)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTriEx-mCherry-LOV2 I427T

    Plasmid

    #81035

    Purpose

    Mammalian expression of LOV I427T, a mutant with super short relaxation time

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Insert

    LOV I427T
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    I427T

    Promoter

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDM

    Plasmid

    #110740

    Purpose

    Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

    Depositor

    Tim Richmond

    Article

    Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pUASTLOTattB_VHH-GFP4::CD8::mCherry

    Plasmid

    #163917

    Purpose

    Expression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancers

    Depositor

    Markus Affolter

    Article

    Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

    Insert

    Fusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
    

    Use

    Cre/Lox

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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