4,908 results

1. U6b

   Plasmid

   #117222

   Purpose

   Expresses gRNA in Aedes aegypti

   Depositor

   Omar Akbari

   Article

   Li et al Elife. 2020 Jan 21;9. pii: 51701. doi: 1

   Insert

   HR U6b promoter-gRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6

   Available since

   Jan. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. ppBAalbNixE1E2 OpIE2-GFP

   Plasmid

   #173505

   Purpose

   transgenesis vector for Aedes albopictus ectopic expression of the Nix gene

   Depositor

   Eric Marois

   Article

   Lutrat et al Commun Biol. 2022 Mar 7;5(1):210. doi: 1

   Inserts

   Nix masculinisation gene
   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Nix isoforms with exons 1, 2 separated by intron 1

   Promoter

   OpIE2 and own

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScarlessHD-C-3xVHH05-DsRed

   Plasmid

   #171580

   Purpose

   For scarless insertion of 3xVHH05 NanoTag into C-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Hidde Ploegh, Norbert Perrimon

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3xVHH05 (UBE2J1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mPA-GFP-PA-Rac1

   Plasmid

   #22022

   Purpose

   Expression of photoactivatable Rac1

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   PA-Rac1 (RAC1 Human, Avena sativa (oat))
   

   Use

   Tags

   6X His and mPA-GFP

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Promoter

   CMV, p10

   Available since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pJFRC-20XUAS-IVS-PhiC31

   Plasmid

   #133565

   Purpose

   Replace the 20XUAS promoter to express PhiC31 under the control of any promoter of interest.

   Depositor

   Tom Clandinin

   Article

   Isaacman-Beck et al Nat Neurosci. 2020 Sep;23(9):1168-1175.

   Insert

   PhiC31 Recombinase (int )
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   20X UAS

   Available since

   Oct. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pOPINTF

   Plasmid

   #41114

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMT-Diaphanous-GFP

   Plasmid

   #24287

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Diaphanous (dia Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 10, 2010

   Availability

   Academic Institutions and Nonprofits only

8. p{Mhc-F4-678-eGFP::FRT,attB}

   Plasmid

   #182185

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. p{ie1-DsRed::FRT-RC,attB}

   Plasmid

   #182191

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   DsRed
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1-TEV-mGFP-StrepII (Human autophagy E3(mGFP)-like enzyme)

    Plasmid

    #169077

    Purpose

    Insect codon optimized ATG genes (excluded tags): 10xHis-TEV-ATG5, ATG7, ATG10, ATG12 and ATG16L1-GFP-TEV-StrepII

    Depositor

    Sascha Martens

    Article

    Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

    Inserts

    ATG5 (ATG5 Human)
    ATG7 (ATG7 Human)
    ATG10 (ATG10 Human)
    ATG12 (ATG12 Human)
    ATG16 (ATG16L1 Human)
    

    Use

    Tags

    GFP tag, GGlinker, TEV cleavage site. StrepII and…

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Polyhedrin promoter

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCasper_Pdf-LexA::VP16

    Plasmid

    #33259

    Depositor

    Michael Rosbash

    Article

    Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

    Insert

    LexA::VP16-SV40 3'UTR (lexA E. coli)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Pdf

    Available since

    Dec. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pAC-QF

    Plasmid

    #24338

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QF
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 31, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRISPaint-T2A-FLPo-3xP3-RFP

    Plasmid

    #127561

    Purpose

    CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-FLPo-SV40 and 3xP3-RFP visible eye marker.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

    Insert

    FLPo
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBacDual with PA+Histag

    Plasmid

    #84870

    Purpose

    Baculovirus Expression of Bacillus anthracis protective antigen

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Insert

    PA
    

    Use

    Baculovirus

    Tags

    His

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. UAS-myr::4xSNAPf

    Plasmid

    #87637

    Purpose

    For creating drosophila transgenics expressing UAS-myr4x::SNAPf

    Depositor

    Gregory Jefferis

    Article

    Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

    Insert

    myr::4xSNAPf
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAct-GW-HygroR

    Plasmid

    #149610

    Purpose

    Gateway compatible expression plasmid for Drosophila and establishing stable cell lines by Hygromycin selection. Actin5c ubiquitous promoter.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    Gateway cassette (ccdb + Chlor.R.)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Act5c

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. p2Bac.XPC

    Plasmid

    #39204

    Depositor

    Aziz Sancar

    Article

    Reardon et al J Biol Chem. 1996 Aug 9;271(32):19451-6.

    Insert

    XPC (XPC Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Q475K polymorphism

    Promoter

    Pp10

    Available since

    July 30, 2012

    Availability

    Academic Institutions and Nonprofits only

18. pOmnibac_ZZ_linker_ybbR_Tau

    Plasmid

    #159717

    Purpose

    Expresses tau in Sf9 cells with a ZZ affinity tag and a ybbR tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    Microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    ZZ affinity tag, ybbR tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFB1.HMBP.PrS.SUZ12

    Plasmid

    #125162

    Purpose

    Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

    Depositor

    Chen Davidovich

    Article

    Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

    Insert

    Polycomb protein SUZ12 (SUZ12 Human)
    

    Use

    Baculovirus expression vector

    Tags

    His-MBP

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pQUASp

    Plasmid

    #46162

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Commun. 2016 Oct 3;7:13010. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge