5,017 results
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Plasmid#185448PurposeCoexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cellsDepositorTagsMBP, YBBR, and ZZExpressionInsectMutationSee Depositor Comments BelowAvailable SinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only
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pJFRC8-40XUAS-IVS-mCD8::GFP
Plasmid#26221DepositorInsertmCD8::GFP
ExpressionInsectAvailable SinceOct. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
UAS-FRT-GFP-FRT-u(M)Cas9
Plasmid#127388PurposeCas9 inducible by removal of a GFP flip-out cassetteDepositorInsertFRT-GFP-FRT-uORF(M)Cas9
UseCRISPRExpressionInsectAvailable SinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
p{CFD4-EYFP-3xP3::DsRed}
Plasmid#86863PurposeExpresses an EYFP gRNA ubiquitously and DsRed in eyes as a marker. With attB integration site.DepositorTypeEmpty backboneExpressionInsectAvailable SinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST-attB-yki:V5-S168A
Plasmid#85617PurposeExpresses V5 tagged mutant yki in Drosophila.DepositorInsertyki-V5-S168A
TagsV5ExpressionInsectAvailable SinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFB1.HMBP.PrS.RBBP4
Plasmid#125164PurposeExpresses human RBBP4 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.DepositorInsertHistone-binding protein RBBP4 (RBBP4 Human)
TagsHis-MBPExpressionInsectPromoterPolyhedrinAvailable SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab9
Plasmid#31736DepositorAvailable SinceSept. 21, 2011AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB_NSlmb-vhhGFP4
Plasmid#35577DepositorInsertNSlmb-vhhGFP4 (slmb Fly)
ExpressionInsectAvailable SinceApril 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFL His-p97
Plasmid#113510PurposeExpression of His-p97 in insect cells (Baculovirus system)DepositorAvailable SinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-OSF-TRIM5alpha(rhesus)
Plasmid#79033PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertOSF-TRIM5alpha(rhesus)
TagsOneSTrEP-FLAGExpressionInsectPromoterpolyhedrinAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_VHH-GFP4::CD8::mCherry
Plasmid#163917PurposeExpression of a membrane-tethered GFP-nanobody marked by mCherry under control of the UAS or LOP enhancersDepositorInsertFusion of vhhGPF4 nanobody with mouse CD8 transmembrane protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectAvailable SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCoofy11
Plasmid#44002DepositorTypeEmpty backboneTagsHis10ExpressionInsectPromoterhr5Available SinceNov. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)
Plasmid#48297DepositorTypeEmpty backboneTagsStrepII and msfGFPExpressionInsectAvailable SinceSept. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDR125
Plasmid#37150DepositorAvailable SinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pB[U6-3 wex2 PUb-DsRed]
Plasmid#169010PurposeFor germline transformation. Expresses white exon 2 sgRNA and DsRed marker in all cells.DepositorInsertwhite ex2 sgRNA
UsePiggybacExpressionInsectPromoterDrosophila melanogaster U6:3Available SinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUASTattB-2xFlag-mCherry-Msp300KASH
Plasmid#170807PurposeExpresses Msp300 KASH domain fused to 2xFLAG and mCherry under Gal4/UAS control for Drosophila transgenesisDepositorInsert2xFLAG/mCherry-Msp300KASH
Tags2xFLAG-mCherry and Msp300KASHExpressionInsectPromoter5xUAS + hsp70 minimal promoterAvailable SinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR]
Plasmid#86449PurposepBAc transposon with transformation marker for Tribolium transgenesis; Drives EGFP expression in the eyes; contains Gal4_ driven by Tribolium heatshock promoter; (Transformation plasmid)DepositorInsert3xP3-EGFP;Tc'hsp5'-Gal4Delta-3'UTR
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBiex-1 Myosin VI HMM_eGFP
Plasmid#82714PurposeExpresses Myosin VI HMM with a C-terminal eGFP.DepositorInsertMyosin VI
TagsFLAG purification tag and eGFPExpressionBacterial and InsectPromoterT7 PromoterAvailable SinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only