4,932 results

1. pHW-CIBN-MP-HFW-CRY2-VHH(GFP)

   Plasmid

   #126027

   Purpose

   express Flag-tagged LARIAT for optogenetic clustering

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CIBN-MP; CRY2 PHR-VHH(GFP)
   

   Tags

   Flag

   Expression

   Insect

   Mutation

   WT

   Promoter

   Hsp70

   Available Since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pattB-nsyb-MKII::GAL4DBDo

   Plasmid

   #64721

   Purpose

   generating transgenic TRIC flies

   Depositor

   Liqun Luo

   Article

   Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

   Insert

   MKIIK11A::GAL4DBDo
   

   Expression

   Insect

   Available Since

   May 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKM377

   Plasmid

   #134210

   Purpose

   Baculovirus expression of CENP-HKM and His-CENP-I

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

   Insert

   CENP-H/K/M untagged + his-I
   

   Expression

   Insect

   Available Since

   Feb. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGMR-QF#7m1

   Plasmid

   #46130

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7m1
   

   Expression

   Insect

   Available Since

   Aug. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKF297

   Plasmid

   #74774

   Purpose

   template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance

   Depositor

   Klaus Foerstemann

   Article

   Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

   Insert

   TEV- 2xV5 tag (short version)
   

   Expression

   Insect

   Available Since

   April 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGP-JFRC59-13XLexAop2-IVS-Syn21-op1-jGCaMP7c-T2A-tdTomato-p10

   Plasmid

   #172918

   Purpose

   LexA activated transgene coexpression of protein calcium sensor and tdTomato under the hsp70 promoter in D. melanogaster

   Depositor

   GENIE Project

   Insert

   jGCaMP7c-T2A-tdTomato
   

   Tags

   6xHis and T2A-tdTomato

   Expression

   Insect

   Promoter

   hsp70

   Available Since

   Sept. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. 13XLexAop2 IVS Jaws mCherry_tr

   Plasmid

   #111551

   Purpose

   Optogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherry

   Depositor

   Vivek Jayaraman

   Article

   Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

   Insert

   Jaws_mCherry_trafficked
   

   Expression

   Insect

   Mutation

   wt

   Promoter

   hsp70

   Available Since

   Sept. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcUW51-gE2

   Plasmid

   #11621

   Depositor

   Pamela Bjorkman

   Article

   Sprague et al J Biol Chem. 2004 Apr 2. 279(14):14184-9

   Insert

   HSV-1 glycoprotein E
   

   Tags

   His

   Expression

   Insect

   Mutation

   deleted amino acids 391-550

   Available Since

   April 6, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pUASTLOTattB_mCherry-CD8-vhhGFP

   Plasmid

   #163930

   Purpose

   Expression of Morphotrap-intracellular (Grab-FP-intracellular) under control of UAS or LOP enhancer

   Depositor

   Markus Affolter

   Article

   Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

   Insert

   Fusion of vhhGPF4 nanobody (intracellular) with mouse CD8 transmembrane protein and mCherry fluorophor
   

   Use

   Cre/Lox

   Tags

   mCherry

   Expression

   Insect

   Available Since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGal80,y+

    Plasmid

    #26851

    Depositor

    Christopher Potter

    Insert

    Gal80 (GAL80 Budding Yeast)
    

    Tags

    Yellow Gene (Drosophila)

    Expression

    Insect

    Available Since

    Dec. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJFRC156-21XUAS-B2RT>-dSTOP-B2RT>-myr::RFP

    Plasmid

    #32135

    Depositor

    Gerald Rubin

    Article

    Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

    Insert

    B2RT> STOP STOP B2RT>
    

    Expression

    Insect

    Available Since

    Nov. 2, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAct:dCas9-scFv

    Plasmid

    #78900

    Purpose

    Expresses scFv-VP64 under actin promoter for SunTag CRISPRa in Drosophila cells

    Depositor

    Norbert Perrimon

    Article

    Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

    Insert

    scFV-VP64
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Insect

    Promoter

    pActin (Drosophila)

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPRxRF3

    Plasmid

    #138386

    Purpose

    TagRFP-T knock-in cassette

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    TagRFP-T
    

    Expression

    Insect

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMT-GFP-p150Dynactin

    Plasmid

    #24283

    Depositor

    Ron Vale

    Article

    Griffis et al J Cell Biol. 2007 Jun 18. 177(6):1005-15

    Insert

    p150 Dynactin (DCTN1-p150 Fly)
    

    Tags

    GFP

    Expression

    Insect

    Available Since

    June 1, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pJC-elav1.8kb-TALE1-VP64-P10

    Plasmid

    #104609

    Purpose

    Expresses TALE1 under the control of a 1.8kb elav enhancer element

    Depositor

    Tudor Fulga

    Article

    Toegel et al Nat Commun. 2017 Nov 21;8(1):1663. doi:

    Insert

    1.8kb elav enhancer element (elav Fly)
    

    Use

    TALEN

    Expression

    Insect

    Promoter

    hsp70

    Available Since

    Jan. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmStaufen_B

    Plasmid

    #145947

    Purpose

    Insect Expression of DmStaufen

    Depositor

    Elisa Izaurralde

    Article

    Behm-Ansmant et al Genes Dev. 2006 Jul 15. 20(14):1885-98.

    Insert

    DmStaufen (stau Fly)
    

    Expression

    Insect

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1161F

    Plasmid

    #183138

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFastBac FPH

    Plasmid

    #1925

    Depositor

    Robert Kingston

    Article

    Francis et al Mol Cell 2001 Sep;8(3):545-56.

    Insert

    polyhomeotic (ph-d, ph-p Fly)
    

    Tags

    Flag

    Expression

    Insect

    Available Since

    Aug. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

    Plasmid

    #192471

    Purpose

    Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

    Insert

    CAHS12
    

    Tags

    AcGFP1

    Expression

    Insect

    Promoter

    Ac5

    Available Since

    Dec. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1025

    Plasmid

    #160235

    Purpose

    FlightN-TetR(OFF)-VP16-Sv40-attB

    Depositor

    Omar Akbari

    Article

    Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

    Inserts

    Tetracycline repressor
    Flightin promoter
    

    Tags

    VP16

    Expression

    Insect

    Available Since

    Nov. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pOPINDsbA

    Plasmid

    #41137

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRB35

    Plasmid

    #72866

    Purpose

    template base vector for N-terminal tagging, constitutive Puromycin resistance

    Depositor

    Klaus Foerstemann

    Article

    Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

    Insert

    base vector for N-terminal tags, Puro
    

    Expression

    Insect

    Available Since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBacPAK8-His-VHL-Flag

    Plasmid

    #29497

    Depositor

    Joan Conaway, Ronald Conaway

    Article

    Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

    Insert

    Von Hippel-Lindau (VHL Human)
    

    Tags

    Flag and His

    Expression

    Insect

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pAcGP67A human LRP6 E3E4 (630–1244)

    Plasmid

    #132628

    Purpose

    expresses human LRP6 E3E4 (630–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (630–1244)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAcGP67A human LRP6 E1E4 (20–1244)

    Plasmid

    #132629

    Purpose

    expresses human LRP6 E1E4 (20–1244) in Hi5 insect cell

    Depositor

    Wenqing Xu

    Article

    Cheng et al Nat Struct Mol Biol. 2011 Oct 9;18(11):1

    Insert

    LRP6 (20–1244)
    

    Expression

    Insect

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFastBacDual with LFn + PrgJ

    Plasmid

    #84869

    Purpose

    Baculovirus Expression of LFn-PrgJ fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein

    Depositor

    Russell Vance

    Article

    Rauch et al J Exp Med. 2016 May 2;213(5):657-65. doi

    Inserts

    LFn
    PrgJ
    

    Use

    Baculovirus

    Tags

    6xHis

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Nov. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCasper-pNos-PCP-eGFP

    Plasmid

    #180274

    Purpose

    to epress PCP-GFP fusion protein in the early fly embryos (driven by maternal nanos driver)

    Depositor

    Hernan Garcia

    Article

    Liu et al PLoS Comput Biol. 2021 May 18;17(5):e100

    Insert

    PCP-eGFP
    

    Tags

    eGFP

    Expression

    Insect

    Promoter

    nanos

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. R-Q111R-N122D

    Plasmid

    #167178

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-R variant with reverted Q111R and N122D mutations in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-R-Q111R-N122D
    ATP1B1
    

    Expression

    Bacterial and Insect

    Mutation

    Changed R at 111 to Q and changed D at 122 to N

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. AT1G66830 X005_pECIA14

    Plasmid

    #114810

    Purpose

    Prey vector AT1G66830 X005_pECIA14 should be used with bait vector AT1G66830 X005_pECIA2.

    Depositor

    Youssef Belkhadir

    Article

    Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

    Insert

    AT1G66830 (AT1G66830 Mustard Weed)
    

    Expression

    Insect

    Promoter

    Metallothionein (Copper-Inducible)

    Available Since

    March 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFAST NPC1(NTD)-3Q

    Plasmid

    #25772

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

    Insert

    npc1 (NPC1 Human)
    

    Tags

    His

    Expression

    Insect

    Mutation

    aa23-252 only; N70Q N122Q N185Q

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

31. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

32. pOPINEneo

    Plasmid

    #53536

    Purpose

    pTriex-based expression vector which adds a C-his8 tag to the inserted gene

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    June 23, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pfast NPC1L1(NTD)

    Plasmid

    #37366

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al PLoS One. 2011 Apr 15;6(4):e18722.

    Insert

    npc1l1 (NPC1L1 Human)
    

    Tags

    His

    Expression

    Insect

    Mutation

    aa22-284only

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pOCC121

    Plasmid

    #118893

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    

    Tags

    HIS6, cleavable with 3C protease and MBP, cleavab…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pDW446

    Plasmid

    #8844

    Depositor

    David Waugh

    Article

    Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

    Type

    Empty backbone

    Tags

    biotin acceptor peptide

    Expression

    Insect

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOPINMalE

    Plasmid

    #41136

    Depositor

    Ray Owens

    Type

    Empty backbone

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pattB-synaptobrevin-14-LEXA-QF-hsp70

    Plasmid

    #46123

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA-QF
    

    Expression

    Insect

    Available Since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pMT-GFP-Patronin

    Plasmid

    #31754

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Patronin (Patronin Fly)
    

    Tags

    GFP

    Expression

    Insect

    Promoter

    Metallothionein

    Available Since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

39. pCa4B2G-QUAS-DSCP-FLPo

    Plasmid

    #24359

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QUAS-DSCP-FLPo
    

    Expression

    Insect

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pQUAST-MKII::GAL4DBDo

    Plasmid

    #64723

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKII::GAL4DBDo
    

    Expression

    Insect

    Available Since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge