4,908 results

1. p-attB-min.hsp70P-FRT-STOP#1-FRT-DamMycLAM

   Plasmid

   #71811

   Purpose

   Generating transgenic flies for FLP-inducible DamID

   Depositor

   Bas van Steensel

   Article

   Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

   Insert

   min.hsp70P-FRT-STOP#1-FRT-Dam-Myc-LAM
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pnos-Fok1:dCas9-nos

   Plasmid

   #62210

   Purpose

   Expresses Fok1:dCas9 under control of nanos promoter and 3'UTR. For germ line restricted high specificity genome engineering in Drosophila melanogaster.

   Depositor

   Simon Bullock

   Insert

   Fok1-dCas9
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   nanos

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASTLOTattB_eGFP::Dpp

   Plasmid

   #163702

   Purpose

   GFP-tagged version of Dpp (Decapentaplegic) under control of UAS and LexO enhancers

   Depositor

   Markus Affolter

   Article

   Harmansa et al Nature. 2015 Nov 19;527(7578):317-22. do

   Insert

   decapentaplegic (dpp Fly)
   

   Use

   Cre/Lox

   Tags

   Tagged with eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGE-attBTT-loxP-DsRed (pTL780)

   Plasmid

   #115160

   Purpose

   Integration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.

   Depositor

   Tobias Langenhan

   Article

   Blanco-Redondo et al G3 (Bethesda). 2018 Jul 31. pii: g3.118.

   Insert

   attB-TT loxP 3xP3-DsRed
   

   Use

   Cre/Lox

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. p3X-UASTattB

   Plasmid

   #189859

   Purpose

   Fly cloning and transformation

   Depositor

   Jung Hwan Kim

   Article

   Singh et al MicroPubl Biol. 2022 Aug 12;2022. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pattB-DSCP-QF#7-hsp70

   Plasmid

   #46133

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFBOH-LIC_DDB1:1-1140_delta:396-689

   Plasmid

   #210897

   Purpose

   Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   loop deletion amino acids I396 to D689

   Promoter

   Polyhedrin

   Available since

   March 19, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pattB-hsp70P-QF#7-hsp70T

   Plasmid

   #46134

   Depositor

   Christopher Potter

   Article

   Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

   Insert

   QF#7
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCRISPaint-T2A-Gal4DBD-3xP3-RFP

   Plasmid

   #127564

   Purpose

   CRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-Gal4DBD-SV40 and 3xP3-RFP visible eye marker.

   Depositor

   Norbert Perrimon

   Article

   Bosch et al Genetics. 2019 Nov 4. pii: genetics.119.

   Insert

   Gal4DBD
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   July 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCoHygro L16_shRNA of spliced Copia

    Plasmid

    #71389

    Purpose

    knock down expression of spliced Copia in Drosophila melanogaster

    Depositor

    Timothy Nilsen

    Insert

    Copia
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    U6

    Available since

    Nov. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pActPL-Gal4AD

    Plasmid

    #15303

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    Gal4AD-ZIP+ (GAL4 )
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. RP125 pUAST EGFP:Orco

    Plasmid

    #53441

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Orco

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Orco (Orco Fly)
    

    Use

    Tags

    EGFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 15, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pOCC292

    Plasmid

    #118881

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
    

    Use

    Tags

    mRuby3, HIS6, cleavable with 3C protease

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS-KS-attB1-2-PT-SA-SD-1-mTagBFP2(11x7)

    Plasmid

    #172065

    Purpose

    Protein trap plasmid for inserting mTagBFP2(11x7) into a phase 1 coding intron

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    mTagBFP2(11x7)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pDCC5

    Plasmid

    #59984

    Purpose

    Bi-cistronic Drosophila CRISPR/Cas9 vector, contains hsp70>Cas9-D10A nickase and U6>sgRNA cassette

    Depositor

    Peter Duchek

    Article

    Gokcezade et al G3 (Bethesda). 2014 Sep 17. pii: g3.114.

    Insert

    Cas9-D10A
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Insect

    Mutation

    D10A

    Promoter

    hsp70Bb

    Available since

    Oct. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. PRE-Hsp70BbCas9_1.2

    Plasmid

    #190796

    Purpose

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.

    Depositor

    Omar Akbari

    Article

    Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

    Insert

    gypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Nov. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC82

    Plasmid

    #118862

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for untagged recombinant protein

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPMW-attB

    Plasmid

    #61814

    Purpose

    pUASp plasmid with attB for Drosophila transgenesis

    Depositor

    Frederique Peronnet

    Article

    Dardalhon-Cumenal et al PLoS Genet. 2018 Jul 11;14(7):e1007498.

    Type

    Empty backbone

    Use

    Tags

    6xMyc

    Expression

    Insect

    Mutation

    Promoter

    UASp

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVL1393-MAL-SYK

    Plasmid

    #157990

    Purpose

    Expresses SYK in insect cell (sf9)

    Depositor

    Byung Il Lee

    Article

    Lee et al Mol Cells. 2018 Jun;41(6):545-552. doi:

    Insert

    Spleen tyrosine kinase (SYK Human)
    

    Use

    Tags

    Met-Ala-Leu and add Leu,Glu residues +His tag

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiex PKC - Peptide 8 GFP RFP

    Plasmid

    #83564

    Purpose

    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.

    Depositor

    Sivaraj Sivaramakrishnan

    Article

    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.

    Insert

    PKC alpha (PRKCA Human)
    

    Use

    Tags

    10 nm ER/K Helix, FLAG purification tag, TEV Prot…

    Expression

    Bacterial and Insect

    Mutation

    Only catalytic domain of PKC (aa 335-672)

    Promoter

    T7 Promoter

    Available since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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