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Plasmid#64070PurposeN-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)DepositorInsertBICD2N (1-400) (Bicd2 Mouse)
UseTagsGFP and His-ZZ-TCSExpressionInsectMutationCodon optimized for Sf9 expressionPromoterAvailable sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.Flag.p34
Plasmid#39221DepositorInsertp34 (GTF2H3 Human)
UseTagsFlagExpressionInsectMutationPromoterPphAvailable sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGL3-DSCP-Gateway
Plasmid#71507PurposeflySTARR-seq validation vector with Gateway cassette downstream of the firefly luciferase gene and the DSCP core promoterDepositorTypeEmpty backboneUseFlystarr-seq validation vectorTagsluciferaseExpressionInsectMutationPromoterAvailable sinceDec. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2Bac.V5.p62
Plasmid#39218DepositorInsertp62 (GTF2H1 Human)
UseTagsV5ExpressionInsectMutationPromoterPp10Available sinceAug. 24, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKM463
Plasmid#134214PurposeBaculovirus expression of CENP-U and CENP-QDepositorInserthis-CENP-U + Q
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-7-QFBDAD
Plasmid#46096DepositorInsertQF#7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKM498
Plasmid#134215PurposeBaculovirus expression of CENP-T and CENP-WDepositorInserthis-CENPT + CENPW
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC-14-LEXA-QF
Plasmid#46104DepositorInsertLexA-QF
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCaSpeR-act5cB-QF#7m1
Plasmid#46126DepositorInsertQF#7m1
UseTagsExpressionInsectMutationPromoterAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmStaufen_B
Plasmid#145947PurposeInsect Expression of DmStaufenDepositorInsertDmStaufen (stau Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
1161F
Plasmid#183138PurposePlasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.DepositorInsertU6.3-gRNAs[sxl, bTub]
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmFMR1_B
Plasmid#145940PurposeInsect Expression of DmFMR1DepositorInsertDmFMR1 (Fmr1 Fly)
UseTagsExpressionInsectMutationTwo amino acid mutations (N45D and G558R) and thr…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1
Plasmid#192471PurposeTransient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cellsDepositorInsertCAHS12
UseTagsAcGFP1ExpressionInsectMutationPromoterAc5Available sinceDec. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pUASTattb-cAMPr Y205A, R739A
Plasmid#84933PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R739A should increase affinity for cAMP without affecting specificity.DepositorInsertcAMPr Y205A R739A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R739APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67B MBP-tagged human Norrin ( 31–133)
Plasmid#132625Purposeexpresses MBP-tagged human Norrin ( 31–133) in Hi5 insect cellDepositorInsertMBP-Norrin ( 31–133)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUASTattb-cAMPr Y205A, R626A
Plasmid#84932PurposecAMPr under 5x UAS regulation. pUASTTB for phiC site mediated insertion. Y205A mutation inhibits PKA-C kinase activity. R626A should decrease affinity for cAMP without affecting specifity.DepositorInsertcAMPr Y205A R626A
UseTagsExpressionInsectMutationPKA-C Y205A, PKA-R R626APromoter5X UASAvailable sinceMarch 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-GCP2
Plasmid#178068PurposeInsect cell expression of GCP2DepositorInsertGCP2 (TUBGCP2 Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
13XLexAop2 IVS Jaws mCherry_tr
Plasmid#111551PurposeOptogenetics in Drosophila: LexAop2-induced expressionof Jaws mCherryDepositorInsertJaws_mCherry_trafficked
UseTagsExpressionInsectMutationwtPromoterhsp70Available sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
AeU6 3p3 GFP LgRNA
Plasmid#182208PurposeFor generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Aedes aegyptiDepositorInsert3xP3-GFP
UseCRISPRTagsExpressionInsectMutationPromoterU6Available sinceApril 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
907L
Plasmid#160237PurposeFlightN-ttgR-VP16-Sv40-attBDepositorInsertsTtgR repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
1025
Plasmid#160235PurposeFlightN-TetR(OFF)-VP16-Sv40-attBDepositorInsertsTetracycline repressor
Flightin promoter
UseTagsVP16ExpressionInsectMutationPromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHW-CIBN-MP-HFW-CRY2-VHH(GFP)
Plasmid#126027Purposeexpress Flag-tagged LARIAT for optogenetic clusteringDepositorInsertCIBN-MP; CRY2 PHR-VHH(GFP)
UseTagsFlagExpressionInsectMutationWTPromoterHsp70Available sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFTSG
Plasmid#162389PurposeExpression of proteins with a C-terminal thermostable GFP (TGP) tag in insect cellDepositorInsertthermostable GFP (TGP)
UseTagsHisExpressionInsectMutationPromoterAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBacPAK8-ElonginB
Plasmid#29504DepositorInsertElongin B (ELOB Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceApril 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKM377
Plasmid#134210PurposeBaculovirus expression of CENP-HKM and His-CENP-IDepositorInsertCENP-H/K/M untagged + his-I
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUASTLOTattB_mCherry::Nrv1::vhhGFP4
Plasmid#163926PurposeExpression of GrabFP-B-intracellular under control of UAS or LOP enhancerDepositorInsertFusion of vhhGPF4 (intracellular) nanobody with Nrv1 protein and mCherry fluorophor
UseCre/LoxTagsmCherryExpressionInsectMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E3E4 (630–1244)
Plasmid#132628Purposeexpresses human LRP6 E3E4 (630–1244) in Hi5 insect cellDepositorInsertLRP6 (630–1244)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGP67A human LRP6 E1E4 (20–1244)
Plasmid#132629Purposeexpresses human LRP6 E1E4 (20–1244) in Hi5 insect cellDepositorInsertLRP6 (20–1244)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5 dOrai
Plasmid#16368DepositorInsertOrai (olf186-F Fly)
UseTagsV5ExpressionInsectMutationPromoterAvailable sinceNov. 30, 2007AvailabilityAcademic Institutions and Nonprofits only