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5,013 results

Showing: 1291 - 1320 of 5013 results

  2. pBiex PKC - Peptide 8 mCit mCer

    Plasmid
    #83563
    Purpose
    Expresses a mCitrine-mCerulean FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PRKCA (PRKCA Human)
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available Since
    Nov. 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    83563-plasmid-map-sequence-id-159470

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  5. Or1a-Gal4

    Plasmid
    #63019
    Purpose
    To express Gal4 in the pattern of the Drosophila melanogaster odorant receptor Or1a, using the promoter sequences of the gene cloned 5' of Gal4
    Depositor
    Leslie Vosshall
    Article
    Fishilevich et al Curr Biol. 2005 Dec 6;15(23):2086-96.
    Insert
    Drosophila melanogaster Or1a promoter
    Tags
    Drosophila ADH (aa's 1-18) fused to Yeast Ga…
    Expression
    Insect
    Available Since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only

  9. pBIG1b_nsp7-His6-nsp8 (SARS-CoV-2)

    Plasmid
    #169180
    Purpose
    Baculoviral transfer vector to express nsp7-His6-nsp8 fusion protein (SARS-CoV-2) in insect cells
    Depositor
    John Diffley
    Article
    Bertolin et al Biochem J. 2021 Jul 16;478(13):2425-2443
    Insert
    nsp7-His6-nsp8 (ORF1ab Synthetic, SARS-CoV-2)
    Tags
    His6 (internal tag linking nsp7 and nsp8): nsp7-H…
    Expression
    Insect
    Mutation
    Codon optimised for insect cells (Spodoptera frug…
    Promoter
    Polyhedrin
    Available Since
    July 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    169180-plasmid-map-sequence-id-333998

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  10. pTSTEP-SNAPf

    Plasmid
    #72335
    Purpose
    Tissue-specifically swappable (C-terminal TagRFPT-to-SNAPf) genome tagging plasmid with Golic+ selection cassette, for fully transgenic gene targeting (Drosophila melanogaster)
    Depositor
    Avital Rodal
    Article
    Koles et al Biol Open. 2015 Dec 23. pii: bio.016089.
    Insert
    RRS-TagRFPT-p10-[Golic+] RRS-SNAPf
    Use
    CRISPR
    Expression
    Insect
    Available Since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    72335-plasmid-map-sequence-id-132005

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  22. pCoofy63

    Plasmid
    #122015
    Purpose
    Baculovirus vector for parallel SLIC cloning containing N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Insect
    Available Since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122015-plasmid-map-sequence-id-243586

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  24. pOPINO

    Plasmid
    #41140
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial, Insect, and Mamm…
    Available Since
    March 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    41140-plasmid-map-sequence-id-54734

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  27. RBBP4

    Plasmid
    #25350
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RBBP4-13 (RBBP4 Human)
    Use
    Baculovirus expression
    Tags
    His
    Expression
    Insect
    Promoter
    polyhedrin
    Available Since
    Aug. 19, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  29. pCS2+ cMyc Dam_f_GFPoNLS

    Plasmid
    #85820
    Purpose
    iDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.
    Depositor
    Joachim Wittbrodt
    Article
    Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261
    Insert
    cMyc Dam_f_GFPoNLS
    Tags
    cMyc and oNLS (optimized nuclear localization sig…
    Expression
    Bacterial, Insect, Mammalia…
    Mutation
    L122A.
    Available Since
    Jan. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    85820-plasmid-map-sequence-id-170474

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Showing: 1291 - 1320 of 5013 results