4,908 results

1. pTriEX-Antenna-GDI Rac1

   Plasmid

   #83359

   Purpose

   Expression of Antenna-Rac1

   Depositor

   Klaus Hahn

   Article

   Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

   Insert

   Rac1 (RAC1 Human)
   

   Use

   Tags

   FRET Antenna (mCerulean-cpVenus) and His

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   CMV

   Available since

   March 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pScarlessHD-N-3x127D01-DsRed

   Plasmid

   #171579

   Purpose

   For scarless insertion of 3x127D01 NanoTag into N-term; Marked by the visible marker 3xP3-DsRed flanked by piggyBac termini.

   Depositor

   Norbert Perrimon, Hidde Ploegh

   Article

   Xu et al Elife. 2022 Jan 25;11. pii: 74326. doi:

   Insert

   3x127D01 (CXCR2 Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUb dRxCas13d + RxCas13d guide RNA

   Plasmid

   #176304

   Purpose

   Expresses catalytic dead RxCas13d and its associated guide RNA in Drosophila cells

   Depositor

   Jeremy Wilusz

   Article

   Ai et al Nucleic Acids Res. 2022 Mar 4. pii: 6542

   Insert

   RxCas13d
   

   Use

   Tags

   HA tag

   Expression

   Insect

   Mutation

   R239A, H244A, R858A, H863A

   Promoter

   Ubi-p63e

   Available since

   March 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUASTattB-mtdT-3XHA

   Plasmid

   #24355

   Depositor

   Liqun Luo

   Article

   Potter et al Cell. 2010 Apr 30. 141(3):536-48.

   Insert

   mtDT-3XHA
   

   Use

   Tags

   3XHA and mtdT

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastbac-Flag-BRCA1

   Plasmid

   #223228

   Purpose

   Insect expression construct for Flag-tagged BRCA1 protein production and purification

   Depositor

   Weixing Zhao

   Article

   Wang et al Mol Cell. 2023 Oct 19;83(20):3679-3691.e

   Insert

   Breast Cancer 1 (BRCA1 Human)
   

   Use

   Tags

   Flag

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   Aug. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJFRC153-20XUAS-IVS-B2::PEST

   Plasmid

   #32134

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   B2::PEST
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTriEx-mVenus-Zdk1-RhoA Q63L

   Plasmid

   #81046

   Purpose

   mammalian expression of Zdk1 tagged RhoA Q63L

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Inserts

   Zdk1
   RhoA Q63L (RHOA Human)
   

   Use

   Tags

   mVenus

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHRW-CRY2olig-VHH(GFP)

   Plasmid

   #126023

   Purpose

   express RFP-tagged CRY2 PHR E490G fused to anti-GFP nanobody for optogenetics

   Depositor

   Eurico Morais-de-Sá

   Article

   Osswald et al Biomolecules. 2019 Feb 12;9(2). pii: bio

   Insert

   CRY2 PHRolig-VHH(GFP)
   

   Use

   Tags

   RFP

   Expression

   Insect

   Mutation

   CRY2 E490G

   Promoter

   Hsp70

   Available since

   Sept. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTriEx-mCherry-LOV2 V416I

   Plasmid

   #81045

   Purpose

   mammalian expression of LOV V416I

   Depositor

   Klaus Hahn

   Article

   Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

   Insert

   LOV V416I
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   V416I

   Promoter

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCaST-elav-VP16AD

    Plasmid

    #15308

    Depositor

    Benjamin White

    Article

    Luan et al Neuron. 2006 Nov 9. 52(3):425-36.

    Insert

    VP16AD-ZIP+
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 10XUAS-flp-DD

    Plasmid

    #124777

    Purpose

    Expresses flippase fused with destabilizing domain (DD) downstream of a 10XUAS sequence.

    Depositor

    Jing-W Wang

    Article

    Sethi et al Elife. 2017 Nov 15;6. pii: e30327. doi:

    Insert

    TMP-inducible destabilized FLP (FLP-DD)
    

    Use

    Tags

    DD

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. act-AsCpf1

    Plasmid

    #73917

    Purpose

    expression plasmid of AsCpf1

    Depositor

    Simon Bullock

    Article

    Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

    Insert

    hAsCpf1
    

    Use

    Tags

    NLS-3xHA

    Expression

    Insect

    Mutation

    Promoter

    act5C

    Available since

    March 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAc5.1B-EGFP-DmG3BP_G

    Plasmid

    #146349

    Purpose

    Insect Expression of DmG3BP

    Depositor

    Elisa Izaurralde

    Insert

    DmG3BP (rin Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    V632G compared to AF231031.1, exact match to NP_5…

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSN840

    Plasmid

    #184832

    Purpose

    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Inserts

    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    

    Use

    Cre/Lox

    Tags

    His-FLAG and mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    p10 and polyhedrin

    Available since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. LHP1048 - pFB-RSP5

    Plasmid

    #32434

    Depositor

    Linda Hicke

    Insert

    RSP5 (RSP5 Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    P-P10 and P-Pol

    Available since

    Sept. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pUAS-PE2-attB

    Plasmid

    #149550

    Purpose

    Expression of PE2 enzyme under control of the Gal4-regulated UAS promoter. Can be used to generate transgenic flies with white+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    UAS

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOCC72

    Plasmid

    #118866

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV

    Depositor

    PEPC MPI CBG, Marc Bickle

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, mGFP, cleavable…

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINO_H11

    Plasmid

    #184277

    Purpose

    Expresses nanobody H11 in E. coli

    Depositor

    Ray Owens

    Article

    Mikolajek et al Proc Natl Acad Sci U S A. 2022 Aug 2;119

    Insert

    Nanobody H11
    

    Use

    Tags

    His6

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    T7

    Available since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mCherry-Zdk1-RhoA Q63L

    Plasmid

    #81058

    Purpose

    mammalian expression of Zdk1 tagged RhoA Q63L

    Depositor

    Klaus Hahn

    Article

    Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

    Inserts

    Zdk1
    RhoA Q63L (RHOA Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pF3BGX-T2A-lexAp65

    Plasmid

    #138393

    Purpose

    RMCE vector for generating C-terminal T2A-lexA-p65 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-lexAp65
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge