5,013 results

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ArpC1_pLib

Plasmid

#173678

Purpose

ArpC1 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

ArpC1 (ARPC1B Human)

Expression

Insect

Promoter

polyhedrin

Available Since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUAST-attB-Flag-dTSC2 WT

Plasmid

#111807

Purpose

Insect Expression of dTSC2

Depositor

Jun Hee Lee

Article

Kim et al Int J Mol Sci. 2015 Mar 27;16(4):7015-26

Insert

dTSC2 (gig Fly)

Tags

3xFlag

Expression

Insect

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGL2-ptc-lu

Plasmid

#37678

Depositor

Philip Beachy

Article

Chen et al Cell. 1999 Aug 6;98(3):305-16.

Insert

ptc promoter (−758 to +130)

Use

Luciferase

Tags

luciferase

Expression

Insect

Promoter

ptc

Available Since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only
874U=ExuL-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

Plasmid

#159671

Purpose

Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

Depositor

Omar Akbari

Article

Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

Insert

ExuL-NLS-hSpCas9-T2A-GFP

Tags

T2A-GFP

Expression

Insect

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 V416T

Plasmid

#81040

Purpose

mammalian expression of LOV V416T

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2 V416T

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

V416T

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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Jag2ICD-HA pBacPAK8-3

Plasmid

#17332

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged2 (Jag2 Rat)

Tags

HA

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pDW445

Plasmid

#8843

Depositor

David Waugh

Article

Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

Type

Empty backbone

Tags

biotin acceptor peptide

Expression

Insect

Available Since

Nov. 18, 2005

Availability

Academic Institutions and Nonprofits only

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pRB33

Plasmid

#72867

Purpose

template for N-terminal 3x FLAG tag, constitutive Puromycin resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

3xFlag tag

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pSK25

Plasmid

#72853

Purpose

template for C-terminal double-FLAG tag, Puromycin-resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

double Flag

Expression

Insect

Available Since

March 4, 2016

Availability

Academic Institutions and Nonprofits only

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pJET1.2[eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40]

Plasmid

#124069

Purpose

CRISPR/Cas mediated knock-in via non-homologous end-joining in Tribolium; bhsp drives EGFP expression; Dm-ebony and Dm-yellow for linearization; (Transformation plasmid; Black eye marker)

Depositor

Gregor Bucher

Insert

eb-Tc’bhsp-EGFP-2A-Cre; ye-3xP3-gTc’v-SV40

Use

CRISPR and Cre/Lox

Expression

Insect

Promoter

bhsp68

Available Since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac-HS-NLRC4Δcard

Plasmid

#177276

Purpose

Purification of mouse NLRC4 (90-1024) from insect cells

Depositor

Hao Wu

Article

Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

Insert

NLRC4 (Nlrc4 Mouse)

Tags

HIS-SUMO-PreScission

Expression

Insect

Mutation

I586T, N761S, M885V, K915R and F972L- please see …

Promoter

polyhedrin

Available Since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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His-ZZ-TEV-BICDR1

Plasmid

#111859

Purpose

Full length BICDR1 for expression in Sf9 cells. 8xHis-ZZ N-terminal tag, TEV site to cleave 8x-His-ZZ.

Depositor

Andrew Carter

Article

Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

Insert

BICDR1 (Bicdl1 Mouse)

Tags

His-tag, TEV site, and ZZ

Expression

Insect

Available Since

July 30, 2018

Availability

Academic Institutions and Nonprofits only

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pUWR-CadTS

Plasmid

#58325

Purpose

Destination vector for inserting ubi-CadTS to Drosophila melanogaster genome

Depositor

Denise Montell

Article

Cai et al Cell. 2014 May 22;157(5):1146-59. doi: 1

Insert

E-cadherin tension sensor

Expression

Insect

Promoter

poly-ubiquitin

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only
pX4

Plasmid

#183903

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Expression

Insect

Promoter

Aedes aegypti PUb

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pX3

Plasmid

#183904

Purpose

donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

Depositor

Eric Marois

Article

Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

Insert

GFP

Expression

Insect

Promoter

Aedes aegypti Polyubiquitin

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacI/MDR3cysless

Plasmid

#74400

Purpose

expression of MDR3 in insect cells

Depositor

Robert Nakamoto

Insert

MDR3 (Abcb1a Mouse)

Tags

10His

Expression

Insect

Mutation

all Cys to Ala

Promoter

Polyhedrin

Available Since

March 2, 2018

Availability

Academic Institutions and Nonprofits only
pTriEx4-ADCY6-C2 (917-1165 aa)

Plasmid

#113904

Purpose

His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Mutation

Please see Depositor Comments

Promoter

T7, CMV

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx4-ADCY6-C1(306-672 aa)

Plasmid

#113903

Purpose

His-S tagged cytoplasmic catalytic domain 1 of Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Article

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Insert

Adenylate Cyclase 6 cytoplasmic catalytic domain 1 (ADCY6 Human)

Tags

His-S

Expression

Bacterial, Insect, and Mamm…

Promoter

T7, CMV

Available Since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pF3BGX-T2A-GAL80

Plasmid

#138396

Purpose

RMCE vector for generating C-terminal T2A-GAL80 fusion

Depositor

Shu Kondo

Article

Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

Insert

T2A-GAL80

Expression

Insect

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGB-08-09-BECN1

Plasmid

#187991

Purpose

Plasmid for the protein expression of BECN1/Beclin1 in a bacterial and insect expression system. Internal_Reference: SMC1325

Depositor

Sascha Martens

Insert

BECN1 (BECN1 Human)

Expression

Bacterial and Insect

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pUAST-HA-dAtg13

Plasmid

#111802

Purpose

Insect Expression of dAtg13

Depositor

Jun Hee Lee

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

dAtg13 (Atg13 Fly)

Tags

HA

Expression

Insect

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pSK15

Plasmid

#72855

Purpose

template for insertion of attP site (C-terminal tag plasmid design), Blasticidin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

attP site

Expression

Insect

Available Since

March 9, 2016

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H

Plasmid

#146503

Purpose

Insect Expression of DmDCP1_327-372

Depositor

Elisa Izaurralde

Article

Tritschler et al Proc Natl Acad Sci U S A. 2009 Dec 22. 1

Insert

DmDCP1_327-372 (Dcp1 Fly)

Expression

Insect

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pattB-nsyb-MKIIK11A::GAL4DBDo

Plasmid

#64722

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

MKIIK11A::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pBiex1-SYNAC

Plasmid

#82710

Purpose

Synthetic soluble adenylyl cyclase FRET sensor and cAMP generator

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al Mol Pharmacol. 2016 Apr;89(4):407-12. do

Insert

SYNAC (ADCY2, ADCY5 Human)

Tags

FLAG, mCerulean, and mCitrine

Expression

Bacterial and Insect

Promoter

T7

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pBDPR

Plasmid

#61598

Purpose

Transformation vector pBDPR

Depositor

John Carlson

Article

Koh et al Neuron. 2014 Aug 20;83(4):850-65. doi: 1

Type

Empty backbone

Use

Drosophila transformation vector

Expression

Insect

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pKM610

Plasmid

#134218

Purpose

Baculovirus expression of CENP-R

Depositor

Iain Cheeseman

Article

McKinley et al Mol Cell. 2015 Dec 17;60(6):886-98. doi:

Insert

CENP-R untagged

Expression

Insect

Available Since

Feb. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pFastBac-Giardia Dicer

Plasmid

#89146

Purpose

Expression of Giardia Dicer in Sf9 cells

Depositor

Jennifer Doudna

Article

MacRae et al Proc Natl Acad Sci U S A. 2008 Jan 15;10

Insert

Giardia Dicer

Tags

His6-teve

Expression

Insect

Available Since

April 13, 2017

Availability

Academic Institutions and Nonprofits only

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pBacPak-HA-E(y)2

Plasmid

#52288

Purpose

Expresses Drosophila E(y)2

Depositor

Jerry Workman

Article

Mohan et al Genes Dev. 2014 Feb 1;28(3):259-72. doi:

Insert

E(y)2 (e(y)2 Fly)

Tags

HA

Expression

Insect

Available Since

April 24, 2014

Availability

Academic Institutions and Nonprofits only

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CJ52 (cg1849 utr mut)

Plasmid

#17780

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

cg1849 utr fragment mut

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Mutation

mutant version

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only

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