4,958 results
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Plasmid#124526PurposepColE1_AlphaDDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial, Insect, and Mamm…Available SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only
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PRE-Hsp70BbCas9_1.2
Plasmid#190796PurposegypsyPRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements with one gypsy insulator element. Step 2 in cloning process.DepositorInsertgypsyPRE-Hsp70Bb-Cas9-T2A-eGFP
ExpressionInsectAvailable SinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1C-RLuc-A90-HhR_O
Plasmid#147073PurposeInsect Expression of A90-HhRDepositorInsertA90-HhR
ExpressionInsectMutationA127 instead of A90 (see comments below)Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQUAST-FLPo
Plasmid#24357DepositorInsertFLPo
ExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
R989-X05-636: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159553PurposeBaculovirus protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
dBrainbow
Plasmid#29327DepositorInsertUAS-Stop-GFP:HSV-mTFP1:HA-mKO2:V5
ExpressionInsectAvailable SinceNov. 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 I427V
Plasmid#81044Purposemammalian expression of LOV2 I427VDepositorInsertLOV I427V
TagsmCherryExpressionBacterial, Insect, and Mamm…MutationI427VAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDmAlfaTub84b
Plasmid#165781PurposeDrosophila Alpha Tubulin promoterDepositorInsertAlfaTub84b
ExpressionInsectAvailable SinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmG3BP_G
Plasmid#146349PurposeInsect Expression of DmG3BPDepositorInsertDmG3BP (rin Fly)
ExpressionInsectMutationV632G compared to AF231031.1, exact match to NP_5…Available SinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
UAST-LbCas12a
Plasmid#140621PurposeGal4 dependent expression of LbCas12a in DrosophilaDepositorInsertLbCas12a
UseCRISPRExpressionInsectPromoterUASTAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBac-GH146-QF
Plasmid#24353DepositorInsertQF
TagsdsRedExpressionInsectAvailable SinceApril 12, 2010AvailabilityAcademic Institutions and Nonprofits only -
pOCC61
Plasmid#118877Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mGFP-3C-HIS6-stop-HindIII cassette
TagsmGFP, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFastBac HTB DPP9L
Plasmid#166788PurposeInsect cell expression vector for DPP9L (protein purification)DepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-MZT1
Plasmid#178075PurposeInsect cell expression of MZT1DepositorInsertMZT1 (MZT1 Human)
ExpressionInsectAvailable SinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUAST cyto Grx1-roGFP2
Plasmid#64994Purposefor expression of cytosolic Grx1-roGFP2 with the Gal4 expression system in drosophilaDepositorAvailable SinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1_GI.1_Q62C-A140C
Plasmid#162581PurposeExpresses norovirus GI.1 VP1 protein with mutations Q62C-A140C in Sf9 insect cellsDepositorInsertVP1 protein from human norovirus GI.1
ExpressionInsectMutationGlutamine 62 changed to Cysteine and Alanine 140 …PromoterpolyhedringAvailable SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB5-PSMB6-PSMB7-TEV-2xSTII
Plasmid#217896PurposeInsect cell expression of human 20S CP proteasome subunit beta type-5 (PSMB5), beta type-6 (PSMB6), and beta type-7 (PSMB7) with C-terminal Twin-Strep-tagDepositorAvailable SinceSept. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMA5-PSMA6-PSMA7
Plasmid#217894PurposeInsect cell expression of human 20S CP proteasome subunit alpha type-5 (PSMA5), alpha type-6 (PSMA6), and alpha type-7 (PSMA7)DepositorAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-PSMB1-PSMB2-PSMB3-PSMB4-TEV-2xSTII
Plasmid#217897PurposeInsect cell expression of human 20S CP proteasome subunit beta type-1 (PSMB1), beta type-2 (PSMB2), beta type-3 (PSMB3), and beta type-4 (PSMB4) with C-terminal Twin-Strep-tagDepositorTags2xSTIIExpressionInsectAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only