4,908 results

1. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   PEPC MPI CBG, Marc Bickle

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Use

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Mutation

   Promoter

   polH

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUASp YFP Rac1 CA

   Plasmid

   #52235

   Purpose

   Drosophila expression of YFP-Rac1 constitutively active mutant

   Depositor

   Matthew Scott

   Insert

   Rac1 (Rac1 Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Q61L; CA mutation

   Promoter

   GAL4

   Available since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pFAST NPC1(NTD)-3Q

   Plasmid

   #25772

   Depositor

   Johann Deisenhofer

   Article

   Kwon et al Cell. 2009 Jun 26. 137(7):1213-24.

   Insert

   npc1 (NPC1 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   aa23-252 only; N70Q N122Q N185Q

   Promoter

   Available since

   Dec. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pGS-FCER1G-1

   Plasmid

   #109192

   Purpose

   Encodes human full-length FCER1G to be expressed in a baculovirus/insect cell expression system

   Depositor

   Monika Golas

   Article

   Le et al Mol Biotechnol. 2018 Jan;60(1):31-40. do

   Insert

   Human full-length FCER1G, codon-optimized (FCER1G Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   p10

   Available since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIE-C-hα2M-H6

   Plasmid

   #128494

   Purpose

   Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

   Depositor

   F Xavier Gomis-Ruth

   Article

   Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

   Insert

   A2M (R787-N1473) (A2M Human)
   

   Use

   Tags

   His tag (6x)

   Expression

   Insect

   Mutation

   contains R787-N1473

   Promoter

   IE1

   Available since

   Aug. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-HS-NLRC4Δcard

   Plasmid

   #177276

   Purpose

   Purification of mouse NLRC4 (90-1024) from insect cells

   Depositor

   Hao Wu

   Article

   Zhang et al Science. 2015 Oct 23;350(6259):404-9. do

   Insert

   NLRC4 (Nlrc4 Mouse)
   

   Use

   Tags

   HIS-SUMO-PreScission

   Expression

   Insect

   Mutation

   I586T, N761S, M885V, K915R and F972L- please see …

   Promoter

   polyhedrin

   Available since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAC-U63-QtgRNA2.1-8R

   Plasmid

   #170514

   Purpose

   gRNA cloning vector containing a Gal80 Co-CRISPR reporter for tissue-specific CRISPR in somatic tissues.

   Depositor

   Chun Han

   Article

   Koreman et al Proc Natl Acad Sci U S A. 2021 Apr 6;118

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   U6:3

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAWH-Rluc-let-7-A114-N40-HhR

   Plasmid

   #50555

   Purpose

   Expression of luciferase reporter mRNA in S2 cell

   Depositor

   Yukihide Tomari

   Article

   Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

   Insert

   Luciferase-let7-A114-N40-HhR
   

   Use

   Luciferase

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pfast NPC1L1(NTD)

    Plasmid

    #37366

    Depositor

    Johann Deisenhofer

    Article

    Kwon et al PLoS One. 2011 Apr 15;6(4):e18722.

    Insert

    npc1l1 (NPC1L1 Human)
    

    Use

    Tags

    His

    Expression

    Insect

    Mutation

    aa22-284only

    Promoter

    Available since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pDW446

    Plasmid

    #8844

    Depositor

    David Waugh

    Article

    Duffy et al Anal Biochem. 1998 Sep 10. 262(2):122-8.

    Type

    Empty backbone

    Use

    Tags

    biotin acceptor peptide

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti PUb

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Aedes aegypti Polyubiquitin

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPINMalE

    Plasmid

    #41136

    Depositor

    Ray Owens

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop

    Plasmid

    #169369

    Purpose

    Expression of gRNA in Culex quinquefasciatus or other mosquitoes

    Depositor

    Valentino Gantz

    Article

    Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

    Insert

    pVMG0302_Cq-U6-1_2xBbsI_gRNA-Loop
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    CPIJ039653

    Available since

    May 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCa4B2G-QUAS-DSCP-FLPo

    Plasmid

    #24359

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    QUAS-DSCP-FLPo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pF3BGX-T2A-GAL80

    Plasmid

    #138396

    Purpose

    RMCE vector for generating C-terminal T2A-GAL80 fusion

    Depositor

    Shu Kondo

    Article

    Kondo et al Cell Rep. 2020 Jan 7;30(1):284-297.e5. d

    Insert

    T2A-GAL80
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQUAST-MKII::GAL4DBDo

    Plasmid

    #64723

    Purpose

    generating transgenic TRIC flies

    Depositor

    Liqun Luo

    Article

    Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

    Insert

    MKII::GAL4DBDo
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    May 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pattB-synaptobrevin-14-LEXA-QF-hsp70

    Plasmid

    #46123

    Depositor

    Christopher Potter

    Article

    Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

    Insert

    LexA-QF
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT-GFP-Patronin

    Plasmid

    #31754

    Depositor

    Ron Vale

    Article

    Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

    Insert

    Patronin (Patronin Fly)
    

    Use

    Tags

    GFP

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only