4,908 results

1. HeatShock-acNPV_Bxb1_SV40UTR

   Plasmid

   #87641

   Purpose

   For creating drosophila transgenics expressing HeatShock-acNPV_Bxb1_SV40UTR

   Depositor

   Gregory Jefferis

   Article

   Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

   Insert

   Bxb1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1073B(HomeR2) =pBac-[Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]

   Plasmid

   #159677

   Purpose

   Plasmid provides the HomeR#2 gene-drive element harboring a rescue, gRNA#2, and 3xP3-eGFP that can be integrated via pBac and inserted at Pol-γ35 site #2 via HDR.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   [Pol-γ35.HL-decPol-γ35-p10]-[U6-gRNA#2(Pol-γ35)]-[3xp3-GFP-SV40]-Pol-γ35.HR-[Opie2-dsRed-SV40]
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. 1095=Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

   Plasmid

   #159673

   Purpose

   Plasmid supports Cas9 expression in both males and females, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

   Depositor

   Omar Akbari

   Article

   Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

   Insert

   Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
   

   Use

   Tags

   T2A-GFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. psiCheck-2-3 x mir-34

   Plasmid

   #40831

   Purpose

   Luciferase reporter

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   three sites partially complementary to miR-34 (mir-34 Fly)
   

   Use

   RNAi

   Tags

   luciferase

   Expression

   Insect

   Mutation

   Promoter

   SV40

   Available since

   Dec. 3, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pENTR-GFP-Patronin CC+CKK

   Plasmid

   #59048

   Purpose

   for baculovirus expression of Patronin CC+CKK (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Sept. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-GFP-Patronin CC 534–1457

   Plasmid

   #59050

   Purpose

   for baculovirus expression of Patronin CC truncation (using Baculodirect kit from Invitrogen)

   Depositor

   Ron Vale

   Article

   Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   eGFP

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pAW-GFP-2xmiR-34 (pAW-AS-25)

   Plasmid

   #40833

   Purpose

   miRNA sensor used in Drosophila cell lines

   Depositor

   Phillip Zamore

   Article

   Ameres et al Science. 2010 Jun 18;328(5985):1534-9.

   Insert

   Two target sites perfectly complementary to miR-34
   

   Use

   Mirna reporter

   Tags

   EGFP

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pMT-GFP-Pat coiled-coil (cc)

   Plasmid

   #31757

   Depositor

   Ron Vale

   Article

   Goodwin et al Cell. 2010 Oct 15;143(2):263-74.

   Insert

   Patronin (Patronin Fly)
   

   Use

   Tags

   GFP

   Expression

   Insect

   Mutation

   Contains only the coiled-coil domain: aa 535-1457

   Promoter

   Metallothionein

   Available since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

9. pBY2887

   Plasmid

   #23221

   Depositor

   Ralf Baumeister

   Article

   Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

   Insert

   SnAvi
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   Feb. 25, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLIB_GST-nsp13 (SARS-CoV-2)

    Plasmid

    #169190

    Purpose

    Baculoviral transfer vector to express GST-nsp13 (SARS-CoV-2) in insect cells

    Depositor

    John Diffley

    Article

    Zeng et al Biochem J. 2021 Jul 16;478(13):2405-2423

    Insert

    GST-nsp13 (ORF1ab Synthetic, SARS-CoV-2)
    

    Use

    Tags

    GST

    Expression

    Insect

    Mutation

    Codon optimised for insect cells (Spodoptera frug…

    Promoter

    Polyhedrin

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVP16 AD

    Plasmid

    #165822

    Purpose

    VP16 activation domain, requires assembly with TL2-TL4 linker and TL1 DNA binding domain

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    VP16 AD
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFL His-SDS22

    Plasmid

    #113512

    Purpose

    Expression of His-SDS22 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    SDS22 (PPP1R7 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pOCC6

    Plasmid

    #118864

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease, and N-terminal EGFP

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HIS6-3C-EGFP-NotI-ccdB-AscI-stop-HindIII cassette
    

    Use

    Tags

    HIS6, cleavable with 3C protease, EGFP

    Expression

    Insect

    Mutation

    Promoter

    polH

    Available since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUASP dsRed Rab7 CA

    Plasmid

    #44825

    Depositor

    Matthew Scott

    Article

    Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

    Insert

    Rab7 (Rab7 Fly)
    

    Use

    Drosophila

    Tags

    dsRed

    Expression

    Insect

    Mutation

    Q67L (constitutive active)

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pAWH-Rluc-let-7-Poly(A)

    Plasmid

    #50556

    Purpose

    Expression of luciferase reporter mRNA in S2 cell

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al Mol Cell. 2012 Dec 28;48(6):825-36. doi:

    Insert

    Luciferase-let7-Poly(A)
    

    Use

    Luciferase

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUASp YFP Rho1 DN

    Plasmid

    #52231

    Purpose

    Drosophila expression of YFP-Rho1 dominant negative mutant

    Depositor

    Matthew Scott

    Insert

    Rho1 (Rho1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    T19N; DN mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pUASp-FMW-attB-AthHen1

    Plasmid

    #104957

    Purpose

    Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila germline tissues using Gal4 drivers

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    pTrasposase promoter

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSGE_MC19_Trl-2xUAS_dCP

    Plasmid

    #71170

    Purpose

    Luciferase reporter

    Depositor

    Alexander Stark

    Article

    Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

    Insert

    MC19_Trl-2xUAS_dCP
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Insect

    Mutation

    Promoter

    DSCP Drosophila synthetic core promoter

    Available since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. ACVR1 (ACVR1Z-c041)

    Plasmid

    #110269

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    ACVR1 (ACVR1 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin promoter

    Available since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pXN-FBLWLF

    Plasmid

    #26262

    Depositor

    Tom Clandinin

    Article

    Gohl et al Nat Methods. 2011 Mar;8(3):231-7. doi: 1

    Insert

    Multiple cloning site
    

    Use

    Drosophila transgenesis

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Promoter

    Available since

    Feb. 16, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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