78,292 results

1. pLenti-eGFP-dSaCas9-blast

   Plasmid

   #191653

   Purpose

   Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9), eGFP, and the blasticidin resistance gene.

   Depositor

   Moshe Szyf

   Article

   Sapozhnikov et al Sci Rep. 2023 Feb 9;13(1):2280. doi: 10.

   Insert

   eGFP-T2A-dSaCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3XFLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiBiT-FZD3

   Plasmid

   #195841

   Purpose

   Expresses human FZD3 with an N-terminal HiBiT; contains I292V and V495I

   Depositor

   Gunnar Schulte

   Article

   Kozielewicz et al ACS Pharmacol Transl Sci. 2021 May 11;4(

   Insert

   FZD3 (FZD3 Human)
   

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   I292V and V495I

   Promoter

   CMV

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT646 human L1 ORF2p-3xFlag (ORFeus-Hs, CMV promoter) in pCEP4 Puro

   Plasmid

   #213026

   Purpose

   Expresses full length human LINE-1 in human cells (codon optimized ORFeus-Hs), with a C-terminal 3xFlag tag on ORF2

   Depositor

   Martin S. Taylor

   Article

   Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

   Insert

   Human LINE-1 (ORFeus-Hs codon optimized sequence) with ORF2-3xFlag
   

   Tags

   3xFlag (ORF2p)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1(+) Drp1 V2 S600A

   Plasmid

   #46344

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Mutation

   S600A

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1(+) Drp1 V2 S600D

   Plasmid

   #46345

   Depositor

   David Chan

   Article

   Loson et al Mol Biol Cell. 2013 Jan 2.

   Insert

   Drp1 V2 (Dnm1l Mouse)
   

   Expression

   Mammalian

   Mutation

   S600D

   Promoter

   CMV

   Available Since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

6. sReach-mTurquoise2

   Plasmid

   #98840

   Purpose

   Produces a fusion between mTurquoise2 and sReach. It can be used as a positive control for FRET (using FLIM)

   Depositor

   Dorus Gadella

   Article

   Mastop et al Sci Rep. 2017 Sep 20;7(1):11999. doi: 10

   Insert

   sReach-mTurquoise2
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mEmerald-MCM2-N-22

   Plasmid

   #54164

   Purpose

   Localization: Nucleus, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

   Insert

   MCM2
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 10, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pBiFC-VC155

   Plasmid

   #22011

   Purpose

   Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   VC155
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Changed Alanine 206 to Lysine

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. TMEM16F-M522K-peGFP-N1

   Plasmid

   #228579

   Purpose

   Expresses mouse TMEM16F-M522K-eGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Expression

   Mammalian

   Mutation

   M522K plus a 3 amino acid N-terminal truncation (…

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBabe-puro-mIR-31

    Plasmid

    #26088

    Depositor

    Bob Weinberg

    Article

    Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

    Insert

    miR-31 (MIR31 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Sept. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-C1-RhebL1

    Plasmid

    #133766

    Purpose

    Expresses GFP-tagged RhebL1 in mammalian cells

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 (RHEBL1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO/Strep-HA-ZAKalpha_K45A

    Plasmid

    #141194

    Purpose

    Expresses kinase-dead Strep-HA-ZAK alpha in mammalian cells, can be used to make inducible cell line

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    Changed Lysine 45 to Alanine

    Promoter

    CMV

    Available Since

    June 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LentiGuide-puro-NLS-GFP

    Plasmid

    #185473

    Purpose

    Mammalian Expression, Lentiviral vector expressing GFP for two-colour competitive growth assay. Clone guide oligos into BsmBI site.

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pAAV-FLEXfrt-mCherry-KASH

    Plasmid

    #209200

    Purpose

    Flp-dependent expression of mCherry tagged KASH protein domain

    Depositor

    Larry Zweifel

    Insert

    mCherry-KASH
    

    Use

    AAV

    Tags

    KASH localization domain

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA4/TO/Strep-HA-ZAKalpha_deltaSdeltaCTD

    Plasmid

    #141198

    Purpose

    Expresses Strep-HA-ZAKalpha_deltaSdeltaCTD in mammalian cells, can be used to make inducible cell lines

    Depositor

    Simon Bekker-Jensen

    Article

    Vind et al Mol Cell. 2020 Apr 9. pii: S1097-2765(20

    Insert

    MAP3K20 (MAP3K20 Human)
    

    Tags

    Strep, HA

    Expression

    Mammalian

    Mutation

    deletion of last 27 amino acids and amino acids 6…

    Promoter

    CMV

    Available Since

    June 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCX137

    Plasmid

    #229212

    Purpose

    CRISPR array plasmid for NOTCH2 (Spacers 1-24)

    Depositor

    Jennifer Doudna

    Article

    Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

    Insert

    CRISPR array plasmid for NOTCH2 (Spacers 1-24)
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRK-Myc-Smurf2 (CG)

    Plasmid

    #13679

    Depositor

    Ying Zhang

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2001 Jan 30. 9

    Insert

    Smurf2 (C716G) (SMURF2 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Smurf2 with a C416 to G substution.

    Available Since

    Feb. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

18. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mPlum-Alpha-V-Integrin-25

    Plasmid

    #55950

    Purpose

    Localization: Focal Adhesions, Excitation: 590, Emission: 649

    Depositor

    Michael Davidson

    Insert

    Alpha-V Integrin (ITGAV Human)
    

    Tags

    mPlum

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 4, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pCIneo-lambdaN-HA-HsDCP2-E148Q_U

    Plasmid

    #147643

    Purpose

    Mammalian Expression of HsDCP2-E148Q

    Depositor

    Elisa Izaurralde

    Article

    Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

    Insert

    HsDCP2-E148Q (DCP2 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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