78,292 results

1. pAC150-RFP-EGFP-LGALS3

   Plasmid

   #175778

   Purpose

   PiggyBac Vector expressing GFP-RFP-LGALS3

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   LGALS3 (LGALS3 Human)
   

   Expression

   Mammalian

   Available Since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. BPK2660

   Plasmid

   #70709

   Purpose

   Human expression plasmid for SaCas9 sgRNA(84nt in length) (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sa-sgRNA(84)

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP-N1-IL6

   Plasmid

   #111933

   Purpose

   Expresses human interleukin-6 (IL6) fused to GFP in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Verboogen et al J Mol Cell Biol. 2018 Jul 16. pii: 50546

   Insert

   Interleukin 6 (IL6 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells

   Promoter

   CMV

   Available Since

   July 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCXB-EBNA1

   Plasmid

   #41857

   Depositor

   Shinya Yamanaka

   Article

   Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

   Insert

   EBNA-1 (EBNA-1 human herpesvirus 4)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMito-TrxRFP1

   Plasmid

   #98997

   Purpose

   mammalian expression of a biosensor to monitor thioredoxin redox dynamics, mitochondrially locaclized

   Depositor

   Huiwang Ai

   Article

   Fan et al Nat Chem Biol. 2017 Jun 26. doi: 10.1038

   Insert

   TrxRFP1 and mitochondrial localization sequence
   

   Tags

   His-tag and Mitochondria localization sequence

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

   Plasmid

   #31358

   Depositor

   Peter Howley

   Article

   Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

   Insert

   JMJD6 (JMJD6 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Available Since

   Oct. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-DEST53 PINK1 N-GFP

   Plasmid

   #13315

   Depositor

   Mark Cookson

   Article

   Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

   Insert

   PINK1 (PINK1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   Oct. 16, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

   Plasmid

   #83841

   Purpose

   Fluorescent probe for G1/S transition and M/G1 transition

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-MmPeg10rc4

   Plasmid

   #174858

   Purpose

   Expresses recoded mouse Peg10 in mammalian cells

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   recoded Mus musculus PEG10 (Peg10 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNLF1-C_PLCG1:1-1291

    Plasmid

    #211089

    Purpose

    Mammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.

    Depositor

    Cheryl Arrowsmith

    Article

    Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

    Insert

    PLCG1:M1-L1291 (PLCG1 Human)
    

    Tags

    NanoLuc luciferase

    Expression

    Mammalian

    Mutation

    isoform 2. I813T natural variant (VAR_011908)

    Promoter

    CMV

    Available Since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBOBI C-GFP PDZD8

    Plasmid

    #227823

    Purpose

    To amplify the virus that expresses C-GFP PDZD8

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDZD8 (PDZD8 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

    Plasmid

    #163024

    Purpose

    Expression of CRISPRi with U6-gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 polyA, and myc NLS

    Expression

    Mammalian

    Promoter

    hEF-1a

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB_tre_Cas9

    Plasmid

    #126029

    Purpose

    PiggyBac cargo vector expressing doxycycline inducible Cas9

    Depositor

    Mauro Calabrese

    Article

    Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

    Inserts

    humanized S. pyrogenes Cas9
    Hygromycin resistance
    

    Use

    CRISPR; Piggybac

    Expression

    Mammalian

    Promoter

    EF1 alpha and TRE

    Available Since

    June 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmTurquoise2-Mito

    Plasmid

    #36208

    Purpose

    In vivo visualization of the mitochondria (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    MTS (COX8A Human)
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-N SV40 LT

    Plasmid

    #37857

    Depositor

    Karl Munger

    Article

    Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

    Insert

    SV40 LT (SV40gp6 Simian Virus 40)
    

    Use

    Retroviral

    Tags

    Flag and HA

    Expression

    Mammalian

    Promoter

    PKG

    Available Since

    July 23, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid

    #216153

    Purpose

    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments

    Depositor

    Pietro Fratta

    Article

    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi

    Insert

    mScarlet with cryptic splice site and C-terminal FLAG tag
    

    Expression

    Mammalian

    Available Since

    July 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPC-Flag-PML-IV

    Plasmid

    #62804

    Purpose

    expresses the isoform IV of PML (promyelocytic leukemia protein)

    Depositor

    Gerardo Ferbeyre

    Article

    Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

    Insert

    PML-IV (PML Human)
    

    Use

    Retroviral

    Tags

    3X-FLAG

    Expression

    Mammalian

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pX459V2.0-HypaCas9

    Plasmid

    #108294

    Purpose

    pX459 V2.0 (Plasmid #62988) with the N692A, M694A, Q695A, and H698A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1 puro Nodamura B2

    Plasmid

    #17228

    Depositor

    Christopher Sullivan

    Article

    Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

    Insert

    Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
    

    Expression

    Mammalian

    Mutation

    2 silent point mutations

    Available Since

    June 22, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #236406

    Purpose

    Expresses cytosolic low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (cyto).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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