78,292 results

1. pLV UBC msTMEM115-3C-3xHA-P2A-eGFP

   Plasmid

   #214997

   Purpose

   Stably express the GolgiTag (GolgiIP handle) in mammalian cells (mouse ORF). eGFP for cell sorting desired expression levels.

   Depositor

   Wade Harper

   Article

   Hollingsworth et al bioRxiv 2024.04.19.590338

   Insert

   TMEM115 (Tmem115 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Promoter

   UBC

   Available Since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-HcKCR2-EYFP

   Plasmid

   #182022

   Purpose

   Express light-gated potassium channel HcKCR2-EYFP in mammalian cells

   Depositor

   Mingshan Xue

   Article

   Govorunova et al Nat Neurosci. 2022 Jun 20. pii: 10.1038/

   Insert

   HcKCR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Available Since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-puro largeTcDNA

   Plasmid

   #14088

   Depositor

   William Hahn

   Article

   Zhao et al Cancer Cell. 2003 May . 3(5):483-95.

   Insert

   Large T antigen (SV40gp6 simian virus)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Feb. 22, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. FL-fibulin-3-V5 pcDNA4

   Plasmid

   #29701

   Depositor

   Mariano Viapiano

   Article

   Hu et al Mol Cancer Res. 2009 Nov . 7(11):1756-70

   Insert

   EFEMP1 (EFEMP1 Human)
   

   Tags

   V5

   Expression

   Mammalian

   Available Since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3.1-GGamma9-GFP2

   Plasmid

   #140991

   Purpose

   Encodes a G gamma subunit (GNG9) containing GFP2 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

   Depositor

   Bryan Roth

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Insert

   GGamma9-GFP2 (GNGT2 Human)
   

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MTS-mCherry-GFP1-10-Hyg-N1

   Plasmid

   #91957

   Purpose

   Expresses mCherry-GFP1-10 in mammalian cell mitochondria

   Depositor

   Rong Li

   Article

   Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

   Insert

   MTS-mCherry-GFP1-10
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCer-TG2-YFP

   Plasmid

   #85471

   Purpose

   TG2 biosensor that allows for quantitative assessment of TG2 conformational changes in live cells using FRET

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Caron et al PLoS One. 2012;7(8):e44159. doi: 10.1371

   Insert

   hTG2 (TGM2 Human)
   

   Tags

   EYFP and mCerulean

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLEX307-DPP4-blast

   Plasmid

   #158452

   Purpose

   Constitutive lentiviral expression of human DPP4.

   Depositor

   Alejandro Chavez, Sho Iketani

   Insert

   DPP4 (DPP4 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV3flag8SHARPIN

   Plasmid

   #50014

   Purpose

   Mammalian expression of human SHARPIN with a c-terminal 3xFLAG tag, and hygromycin selection.

   Depositor

   Martin Dorf

   Article

   Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

   Insert

   SHANK-associated RH domain interactor (SHARPIN Human)
   

   Tags

   3 x FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 31, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCF806-shRen.713

    Plasmid

    #186712

    Purpose

    Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Non-targeting control shRNA (shRen.713).

    Depositor

    Christof Fellmann

    Article

    Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

    Insert

    shRen.713
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lamp1-mScarlet-I

    Plasmid

    #98827

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-gw

    Plasmid

    #37626

    Purpose

    Non-integrating (episomal) expression vector with Gateway cassette

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Stem Cells. 2012 Nov 29. doi: 10.1002/st

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSico PGK GFP

    Plasmid

    #12093

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes the EGFP gene to be recombined out of the construct, activating shRNA expression.

    Depositor

    Tyler Jacks

    Article

    Ventura et al Proc Natl Acad Sci U S A. 2004 Jul 13. 1

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV G-CEPIA1er

    Plasmid

    #58215

    Purpose

    Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

    Depositor

    Masamitsu Iino

    Article

    Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

    Insert

    G-CEPIA1er
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

    Promoter

    CMV

    Available Since

    July 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CMV-Blast-PIP-FUCCI

    Plasmid

    #138715

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BPK2139

    Plasmid

    #65776

    Purpose

    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    

    Use

    CRISPR

    Tags

    NLS-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. LTR5HS S. aureus scaffold CARGO array

    Plasmid

    #191317

    Purpose

    Contains an array of guide RNAs targeting LTR5Hs elements

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Insert

    Array of guide RNAs
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. HSV-1 UL13

    Plasmid

    #26697

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    UL13
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1-CMV-CFP;UBC-Cre25nt

    Plasmid

    #65727

    Purpose

    Expresses Cre recombinase with a 25 nucleotide extracellular vesicle targeting sequence under the UBC promotor and CFP under the CMV promotor.

    Depositor

    Jacco van Rheenen

    Article

    Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

    Inserts

    CMV promoter driving CFP expression
    UBC promoter driving Cre-25nt expression
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Promoter

    CMV and UBC

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. Anti-GABA(A)R, Alpha1 [N95/35R]

    Plasmid

    #177574

    Purpose

    Mammalian Expression Plasmid of anti-GABA(A)R, Alpha1 (Human). Derived from hybridoma N95/35.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-GABA(A)R, Alpha1 (Homo sapiens) recombinant mouse monoclonal antibody (GABRA1 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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