75,813 results

1. pCAG_Xph20-mRuby2-CCR5TC

   Plasmid

   #135531

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Use

   Tags

   mRuby2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCX137

   Plasmid

   #229212

   Purpose

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)

   Depositor

   Jennifer Doudna

   Article

   Xia et al Nat Biotechnol. 2025 Feb 18. doi: 10.103

   Insert

   CRISPR array plasmid for NOTCH2 (Spacers 1-24)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 18, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. GCEPIA1-SNAPER

   Plasmid

   #172659

   Purpose

   Detect the real-time ER calcium store and local calcium microdomains in live cells.

   Depositor

   Pingyong Xu

   Article

   Luo et al Biophys Rep (2019) 5, 31–42

   Insert

   calmodulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. TFORF2400

   Plasmid

   #144335

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   REXO1 (REXO1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Aug. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIR-CMV-SCN1A-Variant-1-IRES-mScarlet

   Plasmid

   #162278

   Purpose

   Eukaryotic expression of human SCN1A variant 1 isoform. This channel has been modified to be stably maintained in standard bacterial strains.

   Depositor

   Al George

   Article

   DeKeyser et al J Biol Chem. 2021 Jan-Jun;296:100298. do

   Insert

   SCN1A Variant 1, stabilized (SCN1A Human)
   

   Use

   Tags

   IRES-mScarlet

   Expression

   Mammalian

   Mutation

   IVS intron inserted after bp2946. Silent codon c…

   Promoter

   CMV

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. NMD2

   Plasmid

   #216220

   Purpose

   Nonsense mediated decay GFP P2A RFP reporter with two b-globin introns

   Depositor

   Rebecca Voorhees

   Article

   Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

   Insert

   GFP P2A RFP_b-globin intron x2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST26-NAT1-C-HA

   Plasmid

   #195178

   Purpose

   Vector for transient expression of NAT1 gene with C-terminal HA tag, gene flanked by att sites for gateway cloning

   Depositor

   Stephanie Roessler

   Insert

   NAT1 (NAT1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV promoter

   Available since

   Feb. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAV pEF1a-DIO-FLPo-WPRE-hGHpA

   Plasmid

   #87306

   Purpose

   An AAV vector expresses FLPo in a Cre recombinase-dependent manner.

   Depositor

   Li Zhang

   Article

   Zingg et al Neuron. 2017 Jan 4;93(1):33-47. doi: 10.

   Has Service

   AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   Cre-dependent mouse codon-optimized Flp recombinase.
   

   Use

   AAV

   Tags

   N/A

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1(+)IRES GFP

   Plasmid

   #51406

   Purpose

   Expresses GFP from internal ribosome entry sequence, cloning component

   Depositor

   Kathleen_L Collins

   Article

   Schaefer et al J Immunol. 2008 Jun 15;180(12):7804-17.

   Insert

   IRES GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-mStayGold-EFS-Puro

    Plasmid

    #227256

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-EF1A-Puro-hTBK1-EGFP

    Plasmid

    #221553

    Purpose

    Expresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.

    Depositor

    Shawn Ferguson

    Article

    Talaia et al bioRxiv [Preprint]. 2023 Dec 17:2023.12.

    Insert

    TBK1 (TBK1 Human)
    

    Use

    Piggybac

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. GPR_control

    Plasmid

    #216222

    Purpose

    GFP P2A RFP control reporter

    Depositor

    Rebecca Voorhees

    Article

    Inglis et al J Cell Sci. 2023 May 15;136(10):jcs26121

    Insert

    GFP P2A RFP_stop
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-SARS2-Spike

    Plasmid

    #145032

    Purpose

    Express SARS-CoV-2 spike protein with C9 tag at C-terminal in mammalian cells

    Depositor

    Fang Li

    Article

    Shang et al Nature. 2020 Mar 30. pii: 10.1038/s41586

    Insert

    SARS-CoV-2 Spike (S Synthetic)
    

    Use

    Tags

    C9

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 15, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pEGFP-C1-Ataxin3Q84

    Plasmid

    #22123

    Depositor

    Henry Paulson

    Article

    Chai et al Proc Natl Acad Sci U S A. 2002 Jul 9. 99

    Insert

    Ataxin 3 (ATXN3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRX-No-HaloTag7-mGFP-LC3

    Plasmid

    #184901

    Purpose

    Stable expression of HaloTag7-mGFP-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7 and monomeric EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSBbi-RB

    Plasmid

    #60522

    Purpose

    SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and blasticidin resistance gene

    Depositor

    Eric Kowarz

    Article

    Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

    Type

    Empty backbone

    Use

    Transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDY1173_ADAR1p150

    Plasmid

    #193192

    Purpose

    Express full length ADAR1p150

    Depositor

    Jonathan Gootenberg, Omar Abudayyeh

    Article

    Jiang et al Nat Biotechnol. 2022 Oct 27. pii: 10.103

    Insert

    ADAR1p150 (ADAR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. mCherry-ZO1-C-14

    Plasmid

    #55165

    Purpose

    Localization: Tight Junctions, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    ZO1 (TJP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLgw V5-EcoDam

    Plasmid

    #59210

    Purpose

    Mammalian DamID control lentiviral vector for expression of V5-Dam only

    Depositor

    Bas van Steensel

    Article

    Vogel et al Genome Res. 2006 Dec;16(12):1493-504. Ep

    Insert

    EcoDam
    

    Use

    Lentiviral; Damid

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Heat Shock Minimal Promoter and heat shock minima…

    Available since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. mEmerald-Calreticulin-N-16

    Plasmid

    #54023

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    Calreticulin (CALR Human)
    

    Use

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 2, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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