75,901 results

1. pEF-GalNAc-T2-msGFP2

   Plasmid

   #140164

   Purpose

   Expresses the Golgi marker GalnacT2-msGFP2

   Depositor

   Benjamin Glick

   Article

   Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

   Insert

   GalNAc-T2-msGFP2 (GALNT2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   March 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mito-PAGFP

   Plasmid

   #23348

   Purpose

   Expresses a mitochondrial matrix-targeted photoactivable GFP that enables detection and quantification of organelle fusion in living cells.

   Depositor

   Richard Youle

   Article

   Karbowski et al J Cell Biol. 2004 Feb 16. 164(4):493-9.

   Insert

   PAGFP
   

   Use

   Tags

   mito

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAIDFA-EF1a-NmScarlet-mAID

   Plasmid

   #140615

   Purpose

   Expression of OsTIR1F74A and mScarlet-mAID protein in mammalian cells under the control of EF1a promoter

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   OsTIR1 mutated F74A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

   Plasmid

   #102886

   Purpose

   PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1 myc-RNF114

   Plasmid

   #58295

   Purpose

   mammalian expression of myc tagged RNF114

   Depositor

   Francesca Capon

   Article

   Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

   Insert

   RNF114 (RNF114 Human)
   

   Use

   Tags

   his and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2014

   Availability

   Academic Institutions and Nonprofits only

6. CMV-SEAP

   Plasmid

   #24595

   Depositor

   Alan Cochrane

   Insert

   SEAP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. piRFP670-N1

   Plasmid

   #45457

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Has Service

   Cloning Grade DNA

   Insert

   iRFP670
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

   Promoter

   CMV

   Available since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-sgRNA Ef1alpha Puro-T2A-GFP

   Plasmid

   #111596

   Purpose

   sgRNA Ef1alpha Puro-T2A-GFP

   Depositor

   Jonathan Weissman

   Article

   Horlbeck et al Cell. 2018 Aug 9;174(4):953-967.e22. doi

   Insert

   mU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. hsKIF5B(1-560)-HaloTag-FRB

   Plasmid

   #213899

   Purpose

   Imaging of KIF5B fused to HaloTag

   Depositor

   Lukas Kapitein

   Insert

   hsKIF5B(aa1-560)-HaloTag-FRB (KIF5B Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-FOXO3a WT

    Plasmid

    #8360

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pF151 pcDNA3.1(+)SOD1WT

    Plasmid

    #26397

    Depositor

    Elizabeth Fisher

    Article

    Stevens et al PLoS One. 2010 . 5(3):e9541.

    Insert

    SOD1 (SOD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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12. VPS13C^mClover3

    Plasmid

    #118760

    Purpose

    VPS13C internally tagged with mClover3 at residue 1914.

    Depositor

    Pietro De Camilli

    Article

    Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

    Insert

    VPS13C (VPS13C Human)
    

    Use

    Tags

    mClover3 inserted at position aa 1914

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAVS1-Puro-CAG-mNG2(1-10)

    Plasmid

    #206042

    Purpose

    Repair template for the integration of a mNG2(1-10) expression cassette into the AAVS1 locus in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Elife. 2024 Apr 23;12:RP92819. doi: 10.7

    Insert

    AAVS1 homology arms with SA-P2A-Puro marker and CAG-mNG2(1-10) expression cassette
    

    Use

    CRISPR and Synthetic Biology; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. TFORF3104

    Plasmid

    #144580

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NKX2-5 (NKX2-5 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAX APP 695

    Plasmid

    #30137

    Depositor

    Dennis Selkoe, Tracy Young-Pearse

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    wild type splice variant 695

    Promoter

    Available since

    Aug. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV.GFAP.Cre.WPRE.hGH

    Plasmid

    #105550

    Purpose

    AAV expression of Cre from GFAP promoter

    Depositor

    James M. Wilson

    Has Service

    AAV PHP.eB and AAV5

    Insert

    Cre
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. M22: pHR-SFFVp-NLS-iLID::EGFP::FTH1

    Plasmid

    #122147

    Purpose

    Self-assebled 24-mer tagged with NLS, iLID, and EGFP. Upon blue-light activation can bind up to 24 SspB-modified proteins.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    FTH1 (FTH1 Human)
    

    Use

    Lentiviral

    Tags

    NLS-iLID-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. TFORF0005

    Plasmid

    #142525

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    HIF3A (HIF3A Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX459v3

    Plasmid

    #178799

    Purpose

    SpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh (Cas9-2A Puro) and U6 (gRNA)

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. TFORF0019

    Plasmid

    #141478

    Purpose

    Lentiviral vector for overexpressing the IRX4 transcription factor ORF with a unique 24-bp barcode. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    IRX4 (IRX4 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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