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pGL4Luc-RLuc

Plasmid

#64034

Purpose

a bidirectional reporter vector allowing the study of biscistronic promoter

Depositor

Daniel Christophe

Article

Christophe-Hobertus et al Mol Cell Endocrinol. 2010 Apr 12;317(1-2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only

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pMiniT:SEP-GluA1 TKIT donor

Plasmid

#169418

Purpose

Donor DNA for TKIT knockin of SEP to GluA1 in mouse

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

Super ecliptic pHluorin (SEP)

Use

CRISPR

Expression

Mammalian

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Article

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

SUN1 (SUN1 Human)

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available Since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-MCS

Plasmid

#145245

Purpose

Backbone vector expressing GAL4 DNA-binding domain followed by MCS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Type

Empty backbone

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-LifeAct-GFP

Plasmid

#190199

Purpose

Expresses the small actin binding peptide, LifeAct, with a GFP reporter

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

LifeAct-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial and Mammalian

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf6

Plasmid

#10834

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf6 (ARF6 Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pH2rU3_ForInd_Omicron_sinobiological_Spiked21_T7_CMV_ZsGT2APurR

Plasmid

#204146

Purpose

Lentivirus backbone with BA.1 spike under an inducible promoter and constitutive expressed zsGreen-T2A-PuR genes

Depositor

Jesse Bloom

Article

Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

Inserts

SARS-CoV-2 BA.1 variant spike
zsGreen-T2A-PuR

Use

Lentiviral

Expression

Mammalian

Mutation

21 amino acid cytoplasmic tail deletion

Promoter

CMV and TRE3G

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBa.TfR-Halo

Plasmid

#162716

Purpose

Mammalian expression of human TFRC

Depositor

Marvin Bentley

Article

Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

Insert

TfR (TFRC Human) (TFRC Human)

Tags

Halo

Expression

Mammalian

Mutation

none

Promoter

Chicken Beta actin

Available Since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Article

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available Since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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mCherry-CaaX(Hras)

Plasmid

#108886

Purpose

Expression of membrane localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

mCherry-CaaX(Hras)

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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Flag-SENP1

Plasmid

#17357

Depositor

Edward Yeh

Article

Cheng et al Cell. 2007 Nov 2. 131(3):584-95.

Insert

SENP1 (SENP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

March 19, 2008

Availability

Academic Institutions and Nonprofits only

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VHL-NanoLuc Vector

Plasmid

#238827

Purpose

Express VHL-NanoLuc Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

VHL (VHL Human)

Tags

NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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APEX2-NLS

Plasmid

#124617

Purpose

proximity biotinylation

Depositor

Alice Ting

Article

Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

Insert

APEX2-NLS

Expression

Mammalian

Promoter

CMV

Available Since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-iLACCO1

Plasmid

#218821

Purpose

Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with mild affinity

Depositor

Robert Campbell

Article

Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

Insert

iLACCO1

Expression

Mammalian

Available Since

April 30, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA4/BDD-FVIII

Plasmid

#41035

Depositor

Robert Peters

Article

Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

Insert

B domain deleted FVIII (F8 Human)

Expression

Mammalian

Mutation

B domain deletion

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA4 myc PGC-1 alpha

Plasmid

#10974

Purpose

Mammalian expression of PGC-1a with myc and his tags

Depositor

Toren Finkel

Article

Ichida et al J Biol Chem. 2002 Dec 27. 277(52):50991-

Insert

PGC-1 alpha (PPARGC1A Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pRK-flag-GFP11

Plasmid

#78590

Purpose

Expression flag-GFP 11 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

flag-GFP 11

Tags

flag

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2016

Availability

Academic Institutions and Nonprofits only
pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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Human Beta-catenin GFP

Plasmid

#71367

Purpose

mammalian expression of human Beta-catenin GFP

Depositor

Alpha Yap

Article

Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

Insert

Beta-catenin (CTNNB1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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Mitochondrial cpGFP

Plasmid

#215711

Purpose

Expresses cpGFP in the mitochondria

Depositor

Katharine Diehl

Article

Smith et al Cell Chem Biol. 2025 Jan 21:S2451-9456(2

Insert

cpGFP

Use

Lentiviral

Tags

FLAG, HA, and mitochondrial localization sequence…

Expression

Mammalian

Mutation

circularly permuted GFP

Promoter

CMV

Available Since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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