78,002 results

1. pcDNA3.1 NPY pHLeon

   Plasmid

   #235176

   Purpose

   Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

   Depositor

   Kate White

   Article

   Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

   Insert

   NPY-pHLeon (NPY Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-SapI

   Plasmid

   #196084

   Purpose

   (Empty Backbone) Inducible CRISPRi vector conferring puromycin resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Inserts

   dCas9-KRAB-MeCP2 repressor
   hU6 promoter and gRNA scaffold
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Catalytically dead mutant of the Cas9 endonucleas…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-Lifeact_Halo_T2A_EGFP

   Plasmid

   #135445

   Purpose

   Mamalian cell expression of HaloTag7 localized at F-actin and cytosolic EGFP

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

   Insert

   Lifact-HaloTag7 and EGFP
   

   Tags

   Lifact

   Expression

   Mammalian

   Promoter

   CMV/TetO

   Available Since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB-cT3G-cERP2-GATA4

   Plasmid

   #192896

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of GATA4

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   GATA4 (GATA4 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Codon-optimized

   Promoter

   TET3G-miniCMV

   Available Since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pER-mScarlet3_N1

   Plasmid

   #189772

   Purpose

   Construct for labelling endoplasmic reticulum in mammalian cells using ER targeted mScarlet3

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   ER-mScarlet3
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-FLAG

   Plasmid

   #46956

   Purpose

   Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   FLAG tag
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFlagCMV2-EFP

   Plasmid

   #12449

   Depositor

   Dong-Er Zhang

   Article

   Zou et al J Biol Chem. 2006 Feb 17. 281(7):3989-94

   Insert

   EFP (TRIM25 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. CD8-GFP-FKBP

   Plasmid

   #100751

   Purpose

   Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

   Depositor

   Stephen Royle

   Article

   Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

   Insert

   CD8A (CD8A Human)
   

   Tags

   FKBP and GFP

   Expression

   Mammalian

   Mutation

   Truncated

   Promoter

   CMV

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. PH-Akt-GFP

   Plasmid

   #51465

   Purpose

   Is a biosensor for PIP3/PI(3,4)P2

   Depositor

   Tamas Balla

   Article

   Varnai et al J Cell Biol. 1998 Oct 19;143(2):501-10.

   Insert

   AKT (aa 1–164)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. MCP-NMS

    Plasmid

    #210705

    Purpose

    This plasmid express EF1a promoter driven MCP-NMS transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti CMV rtTA3 Hygro (w785-1)

    Plasmid

    #26730

    Purpose

    3rd gen lentiviral reverse tetracycline-controlled transactivator 3 (rtTA3) expression vector, CMV promoter, Hygro 

    Depositor

    Eric Campeau

    Insert

    Tetracycline repressor A3 mutant
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABEpuro-ERBB2

    Plasmid

    #40978

    Depositor

    Matthew Meyerson

    Article

    Greulich et al Proc Natl Acad Sci U S A. 2012 Sep 4;109

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-HaloAKAR2.2-T2A-EGFP

    Plasmid

    #216490

    Purpose

    Expression of chemigenetic PKA biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

    Depositor

    Jin Zhang

    Article

    Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

    Insert

    HaloAKAR2.2
    

    Tags

    T2A-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tet-pLKO-puro-SOX2

    Plasmid

    #47540

    Purpose

    Tet inducible knockdown of SOX2.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    SOX2 shRNA
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MC4R

    Plasmid

    #233630

    Purpose

    encodes the human melanocortin 4 receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    MC4R (MC4R Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. EGFP-WASP

    Plasmid

    #242835

    Purpose

    Encodes human WASP and N-terminal EGFP tag

    Depositor

    Cheng-han Yu

    Article

    Qi et al Cell Mol Life Sci. 2025 Mar 24;82(1):127

    Insert

    WASP (WAS Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N/A

    Available Since

    Aug. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti CMV TetR Blast (716-1)

    Plasmid

    #17492

    Purpose

    3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast 

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    Tetracycline repressor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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18. LRRK2-WT

    Plasmid

    #17609

    Depositor

    Ted Dawson

    Article

    West et al Proc Natl Acad Sci U S A. 2005 Nov 15;10

    Insert

    LRRK2 (LRRK2 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    March 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1-IRES eGFP

    Plasmid

    #128059

    Purpose

    Lentiviral expression of an insert from EF1 promoter and co-expression of EGFP. See Depositor Comments for additional information regarding plasmid sequence.

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA5/FRT/TO_[Cox8a]x2_HaloTag7

    Plasmid

    #175529

    Purpose

    Expression of HaloTag7 at the inner membrane of mitochondria in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Cox8a-HaloTag7
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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