78,002 results

1. pENN226_CRISPRcharm

   Plasmid

   #220834

   Purpose

   CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Str-KDEL_SBP-EGFP-Ecadherin

   Plasmid

   #65286

   Purpose

   synchronize trafficking of Ecadherin from the ER (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Tags

   EGFP and IL-2 Signal Sequence and Streptavidin Bi…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. EGFPC1-huNFATc1EE-WT

   Plasmid

   #24219

   Depositor

   Jerry Crabtree

   Article

   Beals et al Genes Dev. 1997 Apr 1. 11(7):824-34.

   Insert

   NFATc1 (NFATC1 Human)
   

   Tags

   EE and GFP

   Expression

   Mammalian

   Available Since

   Sept. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-hHSF1 WT

   Plasmid

   #71724

   Purpose

   plasmid expressing myc-his tag hHSF1 WT

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   HSF1 (HSF1 Human)
   

   Tags

   MYC 6xHIS

   Expression

   Mammalian

   Available Since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA_Cloning Vector

   Plasmid

   #41824

   Purpose

   An empty gRNA expression vector, used to create a gRNA to a specific sequence.

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   AflII site for Gibson Assembly

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-C1-ADRP

   Plasmid

   #87161

   Purpose

   Fluorescent protein localized to lipid droplets (LDs)

   Depositor

   Elina Ikonen

   Article

   Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

   Insert

   ADRP (PLIN2 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFUGW-FIRE-pHLy

   Plasmid

   #170774

   Purpose

   Ratiometric biosensor expressed with UbC promoter for probing lysosomal pH in mammalian cells

   Depositor

   Aimee Kao

   Article

   Chin et al ACS Sens. 2021 Jun 8. doi: 10.1021/acsse

   Insert

   LAMP1 (LAMP1 Human)
   

   Use

   Lentiviral

   Tags

   mCherry and mTFP1

   Expression

   Mammalian

   Mutation

   84 nucleotide Signal Sequence switched to the beg…

   Promoter

   hUbC

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-tetO-myc T58A

   Plasmid

   #19763

   Depositor

   Konrad Hochedlinger

   Article

   Stadtfeld et al Cell Stem Cell. 2008 Mar 6. 2(3):230-40.

   Insert

   c-myc (MYC Human)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   T58A

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-OptoTGFBRs

   Plasmid

   #118942

   Purpose

   Mammalian expression plasmid of OptoTGFBRs (optogenetically-activated TGFb receptors)

   Depositor

   Won Do Heo

   Article

   Li et al ACS Synth Biol. 2018 Feb 16;7(2):443-451

   Insert

   OptoTGFBRs (Tgfb1, Tgfbr2 Mouse, Mustard Weed)
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. UBC plasmid

    Plasmid

    #169746

    Purpose

    Unregulated "open-loop" UBC promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    EGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-HSF1

    Plasmid

    #32537

    Depositor

    Stuart Calderwood

    Article

    Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

    Insert

    HSF1 (HSF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    see depositor comments below

    Promoter

    CMV

    Available Since

    Oct. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGP-AAV-syn-jGCaMP8s-WPRE

    Plasmid

    #162374

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8s
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    S26M F286Y Q315H

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. SaABE8e

    Plasmid

    #138500

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSaCas9
    

    Expression

    Mammalian

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. mScarlet-I-PXN

    Plasmid

    #112956

    Purpose

    In vivo visualization of focal adhesions (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    PXN
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLNCX2 ER:ras

    Plasmid

    #67844

    Purpose

    4-hydroxytamoxifen inducible ER:ras fusion protein

    Depositor

    Masashi Narita

    Article

    Young et al Genes Dev. 2009 Apr 1;23(7):798-803. doi

    Insert

    ER1a H-RasG12V fusion
    

    Expression

    Mammalian

    Mutation

    G525R renders the HBD largely insensitive to 17ß‐…

    Promoter

    CMV

    Available Since

    Aug. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-BRX-eGFP-NLS

    Plasmid

    #217534

    Purpose

    AAV vector to drive the expression of eGFP under the control of the 4xBRE regulatory element of SMAD1 and miniXon splicing casette transcription factor in vivo

    Depositor

    Peter Scheiffele

    Article

    Okur et al bioRxiv 2023

    Insert

    BMP reporter element and miniXon casette (Smad1 Mouse)
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    BMP Reporter Element

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1 SARS-CoV-2 N

    Plasmid

    #158079

    Purpose

    Expresses SARS-CoV-2 nucleocapsid protein in mammalian cells

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 nucleocapsid (N )
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. lenti-25xUPRE-minP-EGFP

    Plasmid

    #159668

    Purpose

    EGFP reporter plasmid containing 25 tandem repeats of the the unfolded protein response element (UPRE)

    Depositor

    Seiichi Oyadomari

    Article

    Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

    Insert

    25xUPRE-minP-EGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV_CKAR3

    Plasmid

    #238413

    Purpose

    AAV construct with CKAR3 expression under a human synapsin promotor and WPRE3 translation enhancer.

    Depositor

    Haining Zhong

    Article

    Yahiro et al bioRxiv [Preprint]. 2024 Jul 23:2024.07.

    Insert

    CKAR3 (PKC sensor)
    

    Use

    AAV

    Expression

    Mammalian

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFH3.2_mTagBFP2-MS2x12

    Plasmid

    #205558

    Purpose

    Express mTagBFP2-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    mTagBFP2
    

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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