74,957 results

1. His-Precission-NEDD4L_C942S_pCDNA3.1(+)

   Plasmid

   #187729

   Purpose

   Express the catalytically inactive NEDD4L FL in mammalian cells

   Depositor

   Sandra Gabelli

   Article

   Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

   Insert

   NEDD4L FL with C874 mutated to a serine (NEDD4L Human)
   

   Use

   Tags

   His-HRV 3C site

   Expression

   Mammalian

   Mutation

   Cys874 mutated to a serine

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. MYC-hTRAK1

   Plasmid

   #225204

   Purpose

   Expresses MYC-tagged human TRAK1

   Depositor

   Thomas Schwarz

   Article

   Pekkurnaz et al Cell. 2014 Jul 3;158(1):54-68. doi: 10.1

   Insert

   trak1 (TRAK1 Human)
   

   Use

   Tags

   MYC

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBABE 3xHA-MKK6-EE puro

   Plasmid

   #216791

   Purpose

   Mammalian expression of constitutively active human MKK6

   Depositor

   Kevin Janes

   Insert

   MKK6 (MAP2K6 Human)
   

   Use

   Retroviral

   Tags

   3x-HA

   Expression

   Mammalian

   Mutation

   S207E and T211E, constitutively active

   Promoter

   LTR

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. MAC-C Sars-CoV-2 NSP12_P314L

   Plasmid

   #194813

   Purpose

   Sars-CoV-2 NSP12_P314L in MAC-C destination vector

   Depositor

   Markku Varjosalo

   Article

   Varjosalo et al 10 May 2024, Research Square

   Insert

   NSP12_P314L (ORF1ab Sars-CoV-2)
   

   Use

   Tags

   MAC-C

   Expression

   Mammalian

   Mutation

   P314L

   Promoter

   Available since

   May 16, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. UTS2R-DuET

   Plasmid

   #213386

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   UTS2R (UTS2R Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBiT3.1-N_TLE1:1-770

   Plasmid

   #211248

   Purpose

   Mammalian expression of full-length human TLE1. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   TLE1:M1-Y770 (TLE1 Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-Rab13 in pCSDEST2

   Plasmid

   #140879

   Purpose

   Expression of zebrafish GFP-tagged Rab13 in mammalian cells.

   Depositor

   Rob Parton

   Article

   Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

   Insert

   Rab13 (rab13 Zebrafish)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. NDV-HN-SpyTag

   Plasmid

   #160000

   Purpose

   Expresses Newcastle disease virus HN protein (47-575) with C-terminal SpyTag fusion in mammalian cells

   Depositor

   Mark Howarth

   Article

   Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

   Insert

   NDV-HN-SpyTag
   

   Use

   Tags

   His6-tag

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta-actin promoter

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-puro-NKX2-8

   Plasmid

   #75080

   Purpose

   retroviral vector holding human NKX2-8 cDNA

   Depositor

   David Mu

   Article

   Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

   Insert

   NKX2-8 (NKX2-8 Human)
   

   Use

   Retroviral

   Tags

   none

   Expression

   Mammalian

   Mutation

   wild-type, full-length, without native 3' UTR

   Promoter

   Available since

   May 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPRia-v2

    Plasmid

    #84832

    Purpose

    CRISPRi/a V2 library parental plasmid

    Depositor

    Jonathan Weissman

    Article

    Horlbeck et al Elife. 2016 Sep 23;5. pii: e19760. doi:

    Inserts

    sgRNA
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mU6

    Available since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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