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79,966 results

Showing: 5281 - 5300 of 79966 results

  1. Human b4 nicotinic receptor subunit EM construct

    Plasmid
    #163961
    Purpose
    pEZT-BM vector with beta4 gene after CMV promoter, published EM construct
    Depositor
    Ryan Hibbs
    Article
    Gharpure et al Neuron. 2019 Nov 6;104(3):501-511.e6. do
    Insert
    Human beta4 nicotinic acetylcholine receptor subunit EM construct
    Use
    Baculovirus (bacmam) production and mammalian exp…
    Tags
    StrepII tag and apocytochrome b(562)RIL (BRIL)
    Expression
    Mammalian
    Mutation
    Pro341-Ser395 replaced with apocytochrome b(562)R…
    Promoter
    CMV
    Available Since
    Feb. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    163961-plasmid-map-sequence-id-323883

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  10. TFORF0799

    Plasmid
    #141705
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    ATF3 (ATF3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Jan. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    141705-plasmid-map-sequence-id-404521

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  11. hSyn1-FLEX-hChR2-tdtomato

    Plasmid
    #41015
    Depositor
    Karel Svoboda
    Insert
    Channelrhodopsin-2
    Use
    Adenoviral
    Tags
    tdtomato
    Expression
    Mammalian
    Mutation
    codon-optimized (human)
    Promoter
    synapsin
    Available Since
    Nov. 6, 2012
    Availability
    Academic Institutions and Nonprofits only

  14. pLNHA-C1-HsSmaug2_Q

    Plasmid
    #147285
    Purpose
    Mammalian Expression of HsSmaug2
    Depositor
    Elisa Izaurralde
    Insert
    HsSmaug2 (SAMD4B Human)
    Expression
    Mammalian
    Mutation
    one silent mutation compared to the seqeuence giv…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    147285-plasmid-map-sequence-id-421013

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  15. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1α and pTRE3G
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  17. rat APOBEC1 E63A

    Plasmid
    #112861
    Purpose
    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Expression
    Mammalian
    Mutation
    changed Glutamic acid 63 to Alanine
    Promoter
    CMV promoter
    Available Since
    Aug. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    112861-plasmid-map-sequence-id-296442

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  19. Gi3-CASE

    Plasmid
    #168122
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.
    Insert
    GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
    Use
    Luciferase
    Tags
    cpVenus on GNG2
    Expression
    Mammalian
    Mutation
    NLuc is inserted at A114/E115 within GNAI1
    Available Since
    July 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    168122-plasmid-map-sequence-id-332862

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  20. pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

    Plasmid
    #221349
    Purpose
    Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Biotin [IPI-M33] Mouse/Rabbit HC
    Use
    Affinity Reagent/ Antibody
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221349-plasmid-map-sequence-id-436706

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Showing: 5281 - 5300 of 79966 results