78,008 results

1. pLD-puro-MTS-EGFP

   Plasmid

   #190270

   Purpose

   Lentiviral conditional expression of mitochondrial-targeted eGFP in mammalian cells

   Depositor

   Christian Münch

   Article

   Michaelis et al Bio Protoc. 2022 Dec 20;12(24):e4578. do

   Insert

   MTS-EGFP
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   Tight TRE promoter

   Available Since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. hiActTS-GR

   Plasmid

   #79775

   Purpose

   high FRET control for ActTS-GR

   Depositor

   Tetsuya Kitaguchi

   Article

   Yamashita et al Sci Rep. 2016 Jun 23;6:28535. doi: 10.10

   Insert

   hiActTS-GR
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. CYFIP1.mCherry

   Plasmid

   #122051

   Purpose

   Expresses human CYFIP1 in mammalian cells.

   Depositor

   Josef Kittler

   Article

   Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

   Insert

   CYFIP1 (CYFIP1 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcdna3.1 hciap1

   Plasmid

   #8311

   Depositor

   Jon Ashwell

   Article

   Li et al Nature 2002 Mar 21;416(6878):345-7.

   Insert

   cIAP1 (BIRC2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Available Since

   April 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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6. PKC epsilon WT

   Plasmid

   #21240

   Depositor

   Bernard Weinstein

   Article

   Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

   Insert

   PKC epsilon (Prkce Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

7. AAV-EF1A-DIO-PSD95.FingR-eGFP-CCR5TC

   Plasmid

   #126216

   Purpose

   Labeling of excitatory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   PSD95.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Artichoke

   Plasmid

   #73320

   Purpose

   Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   SFFV and EF1a

   Available Since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-N1 hMOG-alpha1-EGFP

   Plasmid

   #126463

   Purpose

   Expression a human MOG (isoform alpha1) EGFP fusion protein in mammalian cells

   Depositor

   Markus Reindl

   Article

   Mader et al J Neuroinflammation. 2011 Dec 28;8:184.

   Insert

   Myelin oligodendrocyte glycoprotein (MOG Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA5FRT/Flag-WDR5

    Plasmid

    #29759

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    WDR5 (WDR5 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pEGFP-C3-Sec8

    Plasmid

    #53758

    Purpose

    pEGFP-C3 plasmid that expresses Sec8

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Sec8 (EXOC4 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pCDH-SIRT2-Flag

    Plasmid

    #102624

    Purpose

    Expresses full length SIRT2 isoform 1 in mammalian cells

    Depositor

    Hening Lin

    Article

    Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

    Insert

    SIRT2 (SIRT2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX855

    Plasmid

    #62887

    Purpose

    N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

    Insert

    SpCas9 (aa 2-535)
    

    Use

    CRISPR

    Tags

    FRB and NES PTK2

    Expression

    Mammalian

    Mutation

    Aspartic acide 10 to Alanine (D10A)

    Promoter

    CBh

    Available Since

    March 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. TP53-KO_gRNA_1

    Plasmid

    #195130

    Purpose

    TP53-targeting gRNA in the LRG2.1 backbone

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    TP53 KO gRNA 1 (TP53 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1_crGE

    Plasmid

    #172933

    Purpose

    Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

    Depositor

    Arnold Boersma

    Article

    Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

    Insert

    CrowdingSensorGE
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. lenti-NLS-miRFP670nano-miRFP670

    Plasmid

    #216131

    Purpose

    Lentiviral vector for expressing a fusion of miRFP670nano and miRFP670 localizing to the nucleus.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Nat Cell Biol. 2024 May;26(5):745-756. d

    Insert

    EF1a-NuclearLocalizationSignal-miRFP670nano-miRFP670-WPRE
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. GZnP2 in pcDNA3.1+

    Plasmid

    #161739

    Purpose

    Cytosolic fluorescent reporter for Zn²⁺ (Kd = 352 pM, ex/em = 488 nm/515 nm)

    Depositor

    Yan Qin

    Article

    Fudge et al ACS Chem Biol. 2018 Jul 20;13(7):1897-19

    Insert

    GZnP2
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_T6B -mCherry

    Plasmid

    #205600

    Purpose

    expresses human T6B peptide fused with mChery in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3HA-hMIB2

    Plasmid

    #33312

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    MIB2 (MIB2 Human)
    

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 26, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid

    #183410

    Purpose

    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    KRAB-dCas9-ecDHFR
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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