78,024 results

1. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   G12D

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lifeact-mCherry

   Plasmid

   #193300

   Purpose

   mammalian transient expression - expression of Lifeact-mCherry

   Depositor

   Klaus Hahn

   Article

   Herron et al Nat Commun. 2022 Jul 27;13(1):4363. doi:

   Insert

   Lifeact
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. B1-INTEGRIN WT

   Plasmid

   #195215

   Purpose

   Mammalian expression of mCherry tagged B1 Integrin. Wild-type.

   Depositor

   Valerie Weaver, David Bryant

   Article

   Paszek et al Cancer Cell. 2005 Sep;8(3):241-54. doi:

   Insert

   ITGB1 (ITGB1 Human)
   

   Expression

   Mammalian

   Available Since

   March 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C3-Sec3

   Plasmid

   #53755

   Purpose

   pEGFP-C3 plasmid that expresses Sec3

   Depositor

   Channing Der

   Article

   Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

   Insert

   Sec3 (EXOC1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 22, 2014

   Availability

   Academic Institutions and Nonprofits only

5. LifeAct-GFP-Fis1

   Plasmid

   #182576

   Purpose

   Expresses LifeAct-GFP targeted to the outer mitochondrial membrane via the Fis1 tail anchor sequence

   Depositor

   Uri Manor

   Article

   Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

   Insert

   LifeAct-GFP-Fis1
   

   Tags

   Fis1 and LifeAct

   Expression

   Mammalian

   Available Since

   April 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. ALKBH5-dCas9

   Plasmid

   #134783

   Purpose

   Expression fusion protein ALKBH5-dCas9 in Mammalian cells

   Depositor

   Shu-Bing Qian

   Article

   Liu et al Nat Chem Biol. 2019 Sep;15(9):865-871. d

   Insert

   ALKBH5 and inactive dCas9
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5 FRT TO GFP11-LRRK2_G2019S

   Plasmid

   #231175

   Purpose

   Expresses GFP-11-LRRK2 G2019S in mammalian cells

   Depositor

   Samara Reck-Peterson

   Article

   Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

   Insert

   GFP11-LRRK2 (G2019S) (LRRK2 Human)
   

   Tags

   GFP11

   Expression

   Mammalian

   Mutation

   G2019S

   Promoter

   CMV

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS2+mScarlet3-C Cloning Vector

   Plasmid

   #220616

   Purpose

   To express a protein of interest fused to the C-terminus of mScarlet3 (unpublished)

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Tags

   mScarlet3 fusion

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CLK1

   Plasmid

   #155458

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   CLK1 (CLK1 Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pC0050-CMV-dPspCas13b-longlinker-ADAR2DD(wt)

    Plasmid

    #103866

    Purpose

    dPspCas13b-ADAR2DD(wt) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA. Longer linker than pC0039.

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GSGGGGS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Available Since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-Cas12i2-T2A-EGFP

    Plasmid

    #188270

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    Cas12i2
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEYFP-C1-Drp1

    Plasmid

    #45160

    Depositor

    Richard Youle

    Article

    Frank et al Dev Cell. 2001 Oct;1(4):515-25.

    Insert

    Drp1 (DNM1L Human)
    

    Tags

    EYFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRK5-HA-Ubiquitin-K6

    Plasmid

    #121151

    Purpose

    Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

    Depositor

    Josef Kittler

    Article

    Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

    Insert

    Ubiquitin C (UBC Human)
    

    Expression

    Mammalian

    Mutation

    All Ks apart from K6 mutated to Rs

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGL3 2 kb prom. CD274

    Plasmid

    #107003

    Purpose

    Modified Luciferase expression vector

    Depositor

    Julian Downward

    Article

    Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

    Insert

    CD274 (CD274 Human)
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-TRE-mRuby2

    Plasmid

    #99114

    Purpose

    An AAV genome with tet-inducible expression of the fluorescent protein mRuby2

    Depositor

    Viviana Gradinaru

    Article

    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

    Insert

    mRuby2
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Aug. 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3 p53 WT

    Plasmid

    #69003

    Purpose

    mammalian expression of wild type human p53

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-VSVM

    Plasmid

    #64086

    Purpose

    Expression vector for Vesicular Stomatitis Virus Indiana strain matrix gene

    Depositor

    Ian Wickersham

    Insert

    VSV matrix protein
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. SYNJ1_BirA_Nterm

    Plasmid

    #221523

    Purpose

    Flag-tagged biotinylation enzyme for co-expression of Synj1

    Depositor

    Alessandro Ori

    Insert

    SYNJ1 (SYNJ1 Human)
    

    Tags

    BirA

    Expression

    Mammalian

    Available Since

    July 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti6.2-3xFLAG-V5-ccdB

    Plasmid

    #87072

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a N-terminal 3xFLAG-V5 tag

    Depositor

    Susan Lindquist, Mikko Taipale

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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