82,530 results
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Plasmid#66212PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertADORA3 (ADORA3 Human)
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pU6-(BbsI)_CBh-Cas9-T2A-mCherry-H1-(BamHI)
Plasmid#64217PurposeExpression vector for sgRNAs cloned into the BbsI sites, shRNAs into BamHI & AflII and for Expression of Cas9 linked to mCherry via T2ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
hU6 promoter; BbsI sites for sgRNA
H1 promoter; BamHI site for shRNA
UseCRISPRTags3xFLAG, NLS, and T2A-mCherryExpressionMammalianPromoterCBh, H1, and U6Available sinceSept. 2, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pT7-V5-SBP-C1-HshnRNPM
Plasmid#64924PurposeExpresses V5- and SBP-tagged hnRNPM in mammalian cellsDepositorAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-ESR1
Plasmid#65211PurposeFull length mammalian expression vector for hESR1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertesr1 (ESR1 Human)
TagsAcGFPExpressionMammalianPromoterCMVAvailable sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-CRY2-iSH2
Plasmid#66839PurposeExpression of a light-inducible PI3-kinase, generated by fusing the iSH2 domain of human p85α (residues 420–615) to mCherry-CRY2. Binds endogenous PI3-kinase p110α catalytic subunits constitutively.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiSH2
TagsmCherry-CRY2ExpressionMammalianPromoterCMVAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Syn-FLEX-rc[Chronos-GFP]
Plasmid#62722PurposeAAV-mediated expression of Chronos-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. Using bGHpA signal.DepositorInsertChronos-GFP
UseAAVTagsGFPExpressionMammalianPromoterhuman synapsin promoterAvailable sinceApril 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls
Plasmid#64113PurposeExpression of St1 dCas9-3XGFP in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XsfGFPExpressionMammalianPromoterCMV-TOAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
YFP-Parkin-IRES-zeo
Plasmid#61728PurposeExpression of human Parkin fused to eYFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertParkin (PRKN Human)
TagseYFPExpressionMammalianAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJW1512
Plasmid#62366PurposeN-terminal 3X FLAG fused to wt BirA - mCherry - sec61ßDepositorInsert3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)
UseLentiviralExpressionMammalianPromoterpTRE2Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-UbC
Plasmid#62806PurposeTo express genes at high levels in neuronal cells. This UbC promoter is more active in neurons than the promoter in CMV-based vectors.DepositorTypeEmpty backboneUseAAV; EucaryoticExpressionMammalianPromoterhUbCAvailable sinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-ICUE3
Plasmid#61623PurposePlasma membrane-targeted FRET biosensor for monitoring cAMPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLyn-ICUE3 (RAPGEF3 Human)
TagsECFP, N-terminal targeting sequence from Lyn kina…ExpressionMammalianPromoterCMVAvailable sinceMarch 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSBbi-RH
Plasmid#60516PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and hygromycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBudORF1-CH
Plasmid#51290Purposeexpresses the codon optimized ORF1 of L1RP with his tagDepositorAvailable sinceJan. 6, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG_mRuby2_smFP OLLAS
Plasmid#59761Purposeencodes multiple copies of an epitope tag OLLAS ( not detected by anti GFP ab)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmRuby2_smFP_OLLAS
ExpressionMammalianPromoterCAGAvailable sinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-Intersectin Long
Plasmid#47395DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertintersectin long (ITSN1 Human)
TagsEGFPExpressionMammalianPromoterCMVAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS-H2B-cerulean
Plasmid#53748PurposeContains a fusion of human histone H2B to cerulean fluorescent protein in the pCS2+ vector.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthistone H2B (H2BC21 Human)
TagsCeruleanExpressionMammalianPromotersimian CMV IE94Available sinceJuly 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mTFP1-C1
Plasmid#54613PurposeLocalization: C1 Cloning Vector, Excitation: 462, Emission: 492DepositorTypeEmpty backboneTagsmTFP1ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mKO2-C1
Plasmid#54494PurposeLocalization: C1 Cloning Vector, Excitation: 551, Emission: 565DepositorTypeEmpty backboneTagsmKO2ExpressionMammalianAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PH-Akt(R25C)-GFP
Plasmid#51466PurposeNon-PIP3 binding control for PH-Btk-GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertAKT (aa 1–164)
TagsEGFPExpressionMammalianMutationR25CPromoterCMVAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MR015-H11-R2-TALEN
Plasmid#51555PurposeExpresses the H11-R2 TALEN in mammalian cells. To cleave the H11 locus, this TALEN was coexpressed with H11-L2 (plasmid #51554).DepositorPublicationInsertH11-R2 TALEN
UseTALENExpressionMammalianAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by