78,024 results

1. myrAkt delta4-129

   Plasmid

   #10841

   Depositor

   Richard Roth

   Article

   Kohn et al J Biol Chem. 1996 Sep 6. 271(36):21920-6

   Insert

   Akt (AKT1 Human)
   

   Tags

   HA and myr

   Expression

   Mammalian

   Mutation

   Deletion of PH domain (aa4-129)

   Promoter

   SV40

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-CSF1R-V5/HIS

   Plasmid

   #201984

   Purpose

   expression of human CSF1R receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   CSF1R receptor tyrosine kinase (CSF1R Human)
   

   Tags

   V5, 6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. piggyBAC-U6-GFP-WPRE

   Plasmid

   #200030

   Purpose

   piggyBAC vector backbone with GFP for cloning DNA Ticker Tapes and U6 for pegRNAs

   Depositor

   Jay Shendure

   Type

   Empty backbone

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   U6, EF1a

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. xABEmax

   Plasmid

   #119813

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Huang et al Nat Biotechnol. 2019 May 20. pii: 10.103

   Insert

   ecTadA-ecTadA*(7.10)-nSpCas9 (xCas9)
   

   Expression

   Mammalian

   Available Since

   May 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-L1PA1CH_blast rescue

   Plasmid

   #69611

   Purpose

   Expresses a blasticidin tagged codon optimized L1PA1 designed to recover inserts

   Depositor

   Astrid Roy-Engel

   Insert

   L1PA1
   

   Tags

   blast rescue cassette

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCVpuro-PCAF

   Plasmid

   #63705

   Purpose

   retroviral expression of human PCAF in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   PCAF (KAT2B Human)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Mutation

   E452G

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV7.1_3xFLAG-LATS2

   Plasmid

   #172987

   Purpose

   constitutive expression of FLAG-tagged LATS2

   Depositor

   Michael Wehr

   Article

   Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

   Insert

   3xFLAG-LATS2 (LATS2 Human)
   

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   Oct. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. PB-CAG::Cytbow

   Plasmid

   #158995

   Purpose

   Multicolor cytoplasmic cell labeling with a genome-integrating piggyBac transposon vector (randomly expressing tdTomato, mTurquoise2 or mEYFP upon Cre recombination)

   Depositor

   Jean Livet

   Article

   Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

   Insert

   Cytbow
   

   Tags

   The default expressed EBFP2 is fused to H2B

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EF1A-DIO-Gephyrin.FingR-eGFP-CCR5TC

   Plasmid

   #126217

   Purpose

   Labeling of inhibitory synapses (green fluorescence) in cre-expressing neurons

   Depositor

   Xue Han

   Article

   Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

   Insert

   Gephyrin.FingR-eGFP-CCR5TC
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1A

   Available Since

   July 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV3flag8HOIL-1L

    Plasmid

    #50016

    Purpose

    Mammalian expression of human HOIL-1L (RBCK1) with a c-terminal 3xFLAG tag, and hygromycin selection.

    Depositor

    Martin Dorf

    Article

    Fu et al Cell Res. 2013 Sep 24. doi: 10.1038/cr.2

    Insert

    RanBP-type and C3HC4-type zinc finger containing 1 HOIL-1L (RBCK1 Human)
    

    Tags

    3 x FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 31, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLEX-uORF-HA-birA*-N-Ras(Q61K)-IRES-Puro

    Plasmid

    #120564

    Purpose

    Expresses HA-birA*-N-Ras Q61K fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Q61K

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBMN mCherry-OPTN(D474N)

    Plasmid

    #119683

    Purpose

    For mammalian expression of UBD mutant OPTN

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    OPTN (OPTN Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D474N

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAV-Syn-PSD95.FingR-eGFP-CCR5TC

    Plasmid

    #125693

    Purpose

    Global labeling of excitatory synapses (green fluorescence)

    Depositor

    Xue Han

    Article

    Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

    Insert

    PSD95.FingR-eGFP-CCR5TC
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    Synapsin

    Available Since

    July 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRE-Lepr_FL-IRES-eGFP

    Plasmid

    #196635

    Purpose

    Doxycycline inducible expression of full length mouse Lepr with eGFP marker

    Depositor

    Elaine Fuchs

    Article

    Yuan et al Nature. 2022 Nov 30. doi: 10.1038/s41586

    Insert

    Lepr (Lepr Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHR CD19-mMegf10 CAR

    Plasmid

    #113013

    Purpose

    for production of lentivirus encoding the CD19 CAR-Pmegf10

    Depositor

    Ron Vale

    Article

    Morrissey et al Elife. 2018 Jun 4;7. pii: 36688. doi: 10

    Insert

    CD19 CAR-P Megf10 intracellular domain (CD19, Megf10 Mouse, Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Sept. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX330-LAMP1

    Plasmid

    #207787

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of LAMP1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of LAMP1 (LAMP1 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6-GG-gRNA

    Plasmid

    #226860

    Purpose

    Human gRNA expression vector backbone with BsmBI Golden Gate site

    Depositor

    Alexis Komor

    Article

    Osgood et al Methods in Enzymology, February 5, 2025

    Insert

    Sp-gRNA
    

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-AAV-syn-jGCaMP8f-WPRE

    Plasmid

    #162376

    Purpose

    AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter

    Depositor

    GENIE Project

    Article

    Zhang et al Janelia Research Campus, Online Resource

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    jGCaMP8f
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    Q315L

    Promoter

    Synapsin

    Available Since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. WT NFAT1

    Plasmid

    #11100

    Depositor

    Anjana Rao

    Article

    Monticelli et al Eur J Immunol. 2002 Oct . 32(10):2971-8.

    Insert

    NFAT1 (Nfatc2 Mouse)
    

    Use

    Retroviral

    Tags

    3xHA and GFP

    Expression

    Mammalian

    Available Since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. SF3B3-1

    Plasmid

    #156004

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    SF3B3 (SF3B3 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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