78,093 results

1. qTAG-N-Solo-mStayGold-TUBA1B

   Plasmid

   #227327

   Purpose

   Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry-NLS

   Plasmid

   #39319

   Depositor

   Martin Offterdinger

   Article

   Micutkova et al Int J Cancer. 2012 Apr 1;130(7):1544-57.

   Insert

   mCherry-3xNLS
   

   Tags

   3xSV40LT NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5-F-AF9

   Plasmid

   #49428

   Purpose

   Mammalian expression of Flag-tagged human AF9

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

   Insert

   AF-9 (MLLT3 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPGK-T7/2-CD44v2-10 (fl)

   Plasmid

   #137824

   Purpose

   expression of CD44 proteins in mammalian cells

   Depositor

   Ursula Gunthert

   Article

   Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

   Insert

   CD44v2-10 (fl) (CD44 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   full length and mutations A282T, T483A, N535D, S5…

   Promoter

   PGK

   Available Since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDEST40-Arl13b11

   Plasmid

   #40873

   Depositor

   Tamara Caspary

   Article

   Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

   Insert

   Arl13b (Arl13b Mouse)
   

   Tags

   His and V5

   Expression

   Mammalian

   Mutation

   no stop codon

   Promoter

   CMV

   Available Since

   Nov. 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-UBR5

   Plasmid

   #52050

   Purpose

   CMV driven mammalian expression of UBR5 with an N-termianl GFP fusion

   Depositor

   Darren Saunders

   Article

   Gudjonsson et al Cell. 2012 Aug 17;150(4):697-709. doi: 1

   Insert

   UBR5 (UBR5 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG5 PPAR alpha

   Plasmid

   #22751

   Depositor

   Bruce Spiegelman

   Insert

   PPARalpha (Ppara Mouse)
   

   Expression

   Mammalian

   Available Since

   Dec. 3, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. mTAGBFP2 Peroxisome visual barcode

   Plasmid

   #158668

   Purpose

   tagging clonal populations with Peroxisomal mTAGBFP2 fluorescent protein. Adding Peroxisome localization signal to mTAGBFP2 WC

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mTAGBFP2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF2882

    Plasmid

    #143653

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    XBP1 (XBP1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCX-Klf5

    Plasmid

    #66657

    Purpose

    For expression of mouse Klf5.

    Depositor

    Barak Cohen

    Article

    Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

    Insert

    Klf5 (Klf5 Mouse)
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7-NSP3SA11

    Plasmid

    #89170

    Purpose

    Express Rotavirus SA11 NSP3 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 NSP3
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.hSynap.iAChSnFR

    Plasmid

    #137950

    Purpose

    Expresses iAChSnFR under hSynapsin promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Has Service

    AAV1

    Insert

    iAChSnFR
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSynanpsin

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBp-FGFR2c-WT

    Plasmid

    #45699

    Depositor

    Matthew Meyerson

    Article

    Liao et al Cancer Res. 2013 Jun 20.

    Insert

    FGFR2 IIIc (FGFR2 Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. PRK5-FLAG-Bag6ΔUBL

    Plasmid

    #61837

    Purpose

    express N-terminally FLAG tagged human Bag6 without UBL domain

    Depositor

    Yihong Ye

    Article

    Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

    Insert

    Bag6 (BAG6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. mKeima-Red-N1

    Plasmid

    #54597

    Purpose

    Localization: N1 Cloning Vector, Excitation: 440, Emission: 620

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    mKeima-Red

    Expression

    Mammalian

    Available Since

    June 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

    Plasmid

    #226574

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-DN-hCUL1-FLAG

    Plasmid

    #15818

    Depositor

    Wade Harper

    Article

    Jin et al Methods Enzymol. 2005;399:287-309. doi:

    Insert

    Cullin1 (CUL1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Dominant negative: N452

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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19. pmCherry-C1 actin-3XNLS P2A mCherry

    Plasmid

    #58475

    Purpose

    Expresses nuclear-targeted human actin, with an mCherry expression reporter after a P2A protease cleavage site, on a CMV promoter

    Depositor

    Dyche Mullins

    Article

    Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

    Inserts

    beta actin (ACTB Human)
    3XNLS
    P2A cleavage site
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pT7-VP4SA11

    Plasmid

    #89165

    Purpose

    Express Rotavirus SA11 VP4 in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Rotavirus SA11 VP4
    

    Expression

    Mammalian

    Promoter

    T7 promotor

    Available Since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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