76,967 results

1. pmCherry-N1-Cofilin-S41E

   Plasmid

   #186751

   Purpose

   Expresses S41E phosphomimetic mCherry-tagged human cofilin in mammalian cells

   Depositor

   Geert van den Bogaart

   Article

   Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

   Insert

   Cofilin-1 (CFL1 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed serine 41 to glutamic acid

   Promoter

   CMV

   Available Since

   March 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNLF1-N_DCAF15:1-600

   Plasmid

   #210958

   Purpose

   Mammalian expression of full-length human DCAF15. N-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

   Insert

   DCAF15:M1-L600 (DCAF15 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Pancortin scFv [K96/7]

   Plasmid

   #190497

   Purpose

   Mammalian Expression of Pancortin scFV. Derived from hybridoma K96/7.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Pancortin (Homo sapiens) recombinant scFV (OLFM1 Mouse)
   

   Use

   Tags

   HA, Sortase, 6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-mitoX4-mVenus

   Plasmid

   #185808

   Purpose

   Mitochondrial targetted (COX8) mVenus

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   mito-mVenus
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. H2A-mTurquoise-CDC42-G12V-deltaCAAX

   Plasmid

   #176094

   Purpose

   Constitutively active, nuclear localized Cdc42

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al J Cell Sci. 2021 Aug 6. pii: 271216. doi

   Insert

   CDC42 (CDC42 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G12V

   Promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX313-Firefly luciferase

   Plasmid

   #118017

   Purpose

   Constitutive expression of Firefly luciferase

   Depositor

   David Root, William Hahn

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available Since

   Jan. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. p38-KTR-mStrawberry reporter

   Plasmid

   #158687

   Purpose

   This plasmid is a p38 pathway reporter. It has CMV promoter driving the expression of p38-KTR fused to mStrawberry-pGK-BSD

   Depositor

   Ravid Straussman

   Article

   Kaufman et al Nat Commun. 2022 May 18;13(1):2725. doi:

   Insert

   mStrawberry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   May 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-EGFP-Tau P301L

   Plasmid

   #46908

   Purpose

   expresses EGFP tagged Tau P301L in mammalian cells

   Depositor

   Karen Ashe

   Article

   Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

   Insert

   Tau P301L (MAPT Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   P301L

   Promoter

   CMV

   Available Since

   July 24, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-PinkyCaMP

   Plasmid

   #232856

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under hSyn promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hSyn

   Available Since

   Feb. 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. lenti sgRNA(MS2)_zeo backbone

    Plasmid

    #61427

    Purpose

    3rd generation lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker. Contains BsmBI sites for insertion of spacer sequences.

    Depositor

    Feng Zhang

    Article

    Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and EF1A

    Available Since

    Dec. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-EGFP-C4-Nrf2

    Plasmid

    #21549

    Depositor

    Yue Xiong

    Article

    Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

    Insert

    nuclear factor (erythroid-derived 2)-like 2 (NFE2L2 Human)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-hMLN

    Plasmid

    #27079

    Purpose

    Non-integrating (episomal) expression of human C-MYC, LIN28 and NANOG

    Depositor

    Shinya Yamanaka

    Article

    Okita et al Nat Methods. 2011 May;8(5):409-12

    Insert

    C-MYC, LIN28, NANOG (MYC Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. 4xCSL-luciferase

    Plasmid

    #41726

    Depositor

    Raphael Kopan

    Article

    Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

    Insert

    4xCSL binding sites
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Contains a multimerized high affinity CSL binding…

    Promoter

    Available Since

    Dec. 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSPgRNA

    Plasmid

    #47108

    Purpose

    Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

    Has Service

    Cloning Grade DNA

    Insert

    SPgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti6.2-ccdB-3xFLAG-V5

    Plasmid

    #87071

    Purpose

    Lentiviral Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

    Depositor

    Mikko Taipale, Susan Lindquist

    Article

    Taipale et al Cell. 2014 Jul 17;158(2):434-48. doi: 10

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    3xFLAG-V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVUT-tTR-KRAB

    Plasmid

    #11651

    Purpose

    Tet-regulated (Tet-on) lentiviral vector for transgene (hUbiquitin promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 3rd generation

    Depositor

    Didier Trono, Patrick Aebischer

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Has Service

    Cloning Grade DNA

    Insert

    hUbiquitin C, GFP, tTR-KRAB, Tet-on
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti-PE2max-BSD

    Plasmid

    #191102

    Purpose

    Lentiviral expression plasmid of PEmax prime editor with P2A-BSD marker

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    PEmax-P2A-BSD
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Changed R221K, N394K, H840A from SpCas9

    Promoter

    EF-1a

    Available Since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+HyPer7-NES

    Plasmid

    #136467

    Purpose

    Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

    Depositor

    Vsevolod Belousov

    Article

    Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

    Insert

    HyPer7
    

    Use

    Tags

    Nuclear export signal from the HIV Rev protein

    Expression

    Mammalian

    Mutation

    Promoter

    CMV, SP6

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentipuro3/TO/V5-GW/EGFP-Firefly Luciferase

    Plasmid

    #119816

    Purpose

    3rd generation lentiviral plasmid for expression of EGFP Firefly Luciferase fusion protein in mammalian cells

    Depositor

    Ethan Abel

    Article

    Lorenzatti Hiles et al Nat Protoc. 2019 Mar;14(3):738-755. doi:

    Inserts

    Luciferase
    Puromycin
    

    Use

    Lentiviral and Luciferase

    Tags

    EGFP and V5

    Expression

    Mammalian

    Mutation

    Stop codon removed in the frame of the V5 frame v…

    Promoter

    CMV/TO and Human Phosphoglycerate kinase

    Available Since

    Jan. 2, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pSpCas9(BB)-2A-Neo

    Plasmid

    #127762

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge