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79,308 results

Showing: 8221 - 8240 of 79308 results

  2. pAAV-EF1a-double floxed-hChR2(H134R)-EYFP-WPRE-HGHpA

    Plasmid
    #20298
    Purpose
    Cre-activated AAV expression of humanized ChR2 with H134R mutation fused to EYFP for optogenetic activation
    Depositor
    Karl Deisseroth
    Has Service
    AAV PHP.eB, AAV Retrograde, AAV1, AAV5, and AAV9
    Insert
    channelrhodopsin-2
    Use
    AAV
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    humanized ChR2 gene with histidine 134 changed to…
    Promoter
    EF1alpha
    Available Since
    May 13, 2009
    Availability
    Academic Institutions and Nonprofits only
    20298-plasmid-map-sequence-id-191904

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  3. TriEx-4 DHX36

    Plasmid
    #68368
    Purpose
    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.
    Depositor
    James Vaughn
    Article
    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2
    Insert
    DHX36 (DHX36 Human)
    Tags
    Histidine
    Expression
    Bacterial, Insect, and Mamm…
    Promoter
    CMV ie enhancer/promoter
    Available Since
    Sept. 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  4. TFORF2538

    Plasmid
    #144036
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    SOX4 (SOX4 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    144036-plasmid-map-sequence-id-400532

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  6. pM162

    Plasmid
    #173221
    Purpose
    Expresses ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP(1-9)-GB1 and Puro-T2A-MCP-mCherry-GFP11-GB1
    Depositor
    Hajin Kim
    Article
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Inserts
    ScFV-GCN4-GFP10M2-GB1-T2A-OptGFP (1-10)-GB1
    MCP-mCherry-GFP11-GB1
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173221-plasmid-map-sequence-id-341549

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  11. pXJ-Myc-IRF8

    Plasmid
    #232668
    Purpose
    transient expresses IRF8 in mammalian cells
    Depositor
    Chen-Yang Shen
    Insert
    IRF8 (IRF8 Human)
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    232668-plasmid-map-sequence-id-464286

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  12. OPRM1-DuET

    Plasmid
    #213361
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    OPRM1 (OPRM1 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    213361-plasmid-map-sequence-id-431826

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  14. TFORF2249

    Plasmid
    #143184
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TCF12 (TCF12 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    June 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    143184-plasmid-map-sequence-id-397810

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  18. Opto-CRAC version 1

    Plasmid
    #101245
    Purpose
    Genetically-encoded calcium actuator; mCherry-tagged LOV2-STIM1 chimera that enables optical control of calcium signals and calcium dependent signaling
    Depositor
    Yubin Zhou
    Article
    He et al Elife. 2015 Dec 8;4. pii: e10024. doi: 1
    Insert
    LOV2 and STIM1 cytosolic fragment
    Tags
    mCherry
    Expression
    Bacterial and Mammalian
    Promoter
    CMV
    Available Since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    101245-plasmid-map-sequence-id-209272

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Showing: 8221 - 8240 of 79308 results