75,901 results

1. pDAS12489_PEAR-GFP_2in1_2.0

   Plasmid

   #177186

   Purpose

   PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HsTPC2-EYFP

   Plasmid

   #135194

   Purpose

   Expresses tagged TPC2 in mammalian cells

   Depositor

   Antony Galione

   Article

   Davis et al Curr Biol. 2012 Dec 18;22(24):2331-7. do

   Insert

   TPC2 (TPCN2 Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFP rbFOX2

   Plasmid

   #63086

   Purpose

   Mammalian expression of EGFP tagged FOX2

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   RBFOX2 (RBFOX2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGW_GPHN.FingR-YFP

   Plasmid

   #46298

   Depositor

   Don Arnold

   Article

   Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

   Insert

   GPHN.FingR (GPHN Synthetic, Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pC1-SypHer3s-IMS

   Plasmid

   #108120

   Purpose

   Mitochondrial ratiometric pH probe with enhanced brightness fused to Smac/DIABLO fragment. Probe is localized in mitochondrial intermembrane space. Mammalian cell expression.

   Depositor

   Vsevolod Belousov

   Article

   Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

   Insert

   SypHer3s-IMS
   

   Use

   Tags

   ratiometricYFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FPR3-Tango

   Plasmid

   #66285

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   FPR3 (FPR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXS-IRES-BLAST TFR1

   Plasmid

   #169890

   Purpose

   Express TFR1 open reading frame. Lacks IRE regulatory elements

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   TFRC (TFRC Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   TFRC cDNA, lacking 3'UTR regulatory elements

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMax_mOrange_G299C

   Plasmid

   #177829

   Purpose

   Site-specific mutagenesis of mOrange for the purposes of creating a site-specific G:G mispair for the purposes of measuring MMR activity via Host Cell Reactivation (HCR).

   Depositor

   Leona Samson, Zachary Nagel

   Article

   Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

   Insert

   mOrange_G299C
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G299C

   Promoter

   CMV

   Available since

   Nov. 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. EK0479 CMV-TO-VEGF-A(165) (FLP-IN)

   Plasmid

   #233384

   Purpose

   Secreted human VEGF-A

   Depositor

   Xiaojing Gao

   Article

   Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

   Insert

   VEGF-A (VEGFA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available since

   April 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid

    #207764

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pPB-CAG-ECC-Biosensor

    Plasmid

    #140832

    Purpose

    Encoding the CAG promoter based eccDNA biosensor

    Depositor

    Yonglun Luo

    Article

    Moller et al Nucleic Acids Res. 2018 Dec 14;46(22):e1

    Insert

    ECC-Biosensor
    

    Use

    Piggybac (pb) transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF1438

    Plasmid

    #143807

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    EP400 (EP400 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP-Atg14L

    Plasmid

    #21635

    Depositor

    Tamotsu Yoshimori

    Article

    Matsunaga et al Nat Cell Biol. 2009 Apr . 11(4):385-96.

    Insert

    Atg14L (ATG14 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB-GFP1-10-HygR

    Plasmid

    #223675

    Purpose

    To check the split GFP signals in mitochondria (1)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3/HA-FLAG-RBP2

    Plasmid

    #14800

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide1

    Plasmid

    #118169

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lenti-(BB)-hPGK-KRAB-dCas9-P2A-BlastR TCF7L2 TSS-guide2

    Plasmid

    #118170

    Purpose

    CRISPR-mediated repression of TCF7L2. KRAB domain and catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the hPGK promoter.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK and U6

    Available since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmCherry-HP1alpha

    Plasmid

    #179703

    Purpose

    visualization of centromere heterochromatin

    Depositor

    Kenji Sugimoto

    Article

    Fukada et al Biosci Biotechnol Biochem. 2019 Jul;83(7

    Insert

    heterochromatin protein alpha (CBX5 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCIneo-V5-SBP-HseIF4AI_AC

    Plasmid

    #148438

    Purpose

    Mammalian Expression of HseIF4AI

    Depositor

    Elisa Izaurralde

    Article

    Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

    Insert

    HseIF4AI (EIF4A1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    3 silent mutations relative to NM_001416.2

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-mScarlet-I

    Plasmid

    #159172

    Purpose

    To express a bright monomeric red FP for labelling and detection of mammalian cells

    Depositor

    Pantelis Tsoulfas

    Insert

    mScarlet-I
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Codons were optimized to Human codon usage

    Promoter

    CMV

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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