78,128 results

1. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

   Plasmid

   #111925

   Purpose

   Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

   Depositor

   Takeharu Nagai

   Article

   Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

   Insert

   CeNL(Ca2+)_110μ
   

   Tags

   Calreticulin and KDEL

   Expression

   Mammalian

   Mutation

   E31D, F92W, E104D. D133E at CaM

   Promoter

   CMV

   Available Since

   June 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. qTAG-C-miRFP670nano3-Blast

   Plasmid

   #207713

   Purpose

   Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Blast cassette

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCI-HA3-HPS1

   Plasmid

   #133930

   Purpose

   Expresses 3xHA-HPS1 construct in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Martina et al J Biol Chem. 2003 Aug 1;278(31):29376-84

   Insert

   HPS1 (HPS1 Human)
   

   Tags

   3xHA tag

   Expression

   Mammalian

   Promoter

   CMV I.E.

   Available Since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-Tuba

   Plasmid

   #22214

   Depositor

   Pietro De Camilli

   Article

   Salazar et al J Biol Chem. 2003 Dec 5. 278(49):49031-4

   Insert

   Tuba (DNMBP Human)
   

   Tags

   HA

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 9, 2009

   Availability

   Academic Institutions and Nonprofits only

5. pFH3.29_Cre

   Plasmid

   #205560

   Purpose

   Express Cre (without export tag)

   Depositor

   Michael Elowitz

   Article

   Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

   Insert

   Cre
   

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas-hHDAC3-R265P

   Plasmid

   #103786

   Purpose

   Expresses dCas9 fused to human HDAC3 with R265P mutation

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCas9-HDAC3-R265P (HDAC3 Human, Synthetic, S. pyogenes)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   Changed arginine 265 to proline

   Promoter

   EF1A

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. ChlorON-3

   Plasmid

   #193479

   Purpose

   Turn-on fluorescent sensor for chloride

   Depositor

   Sheel Dodani

   Article

   Tutol et al ACS Cent Sci. 2023 Dec 18;10(1):77-86. d

   Insert

   ChlorON-3
   

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV/TO GFP-Zeo DEST (719-1)

   Plasmid

   #17431

   Purpose

   3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, GFP-Zeo

   Depositor

   Eric Campeau, Paul Kaufman

   Article

   Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Destination vector

   Expression

   Mammalian

   Available Since

   Aug. 12, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti-CMV-FlaviA-mNeptune-puro

   Plasmid

   #140091

   Purpose

   Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   FlaviA-mNeptune
   

   Use

   Lentiviral; High expression

   Tags

   mNeptune

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. FLAG-mBAF45a IRES GFP

    Plasmid

    #17880

    Depositor

    Jerry Crabtree

    Article

    Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

    Insert

    BAF45a (Phf10 Mouse)
    

    Tags

    FLAG and IRES EGFP

    Expression

    Mammalian

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZFN-AAVS1_ELD

    Plasmid

    #159297

    Purpose

    ZFN (left) targeting the AAVS1 locus with obligate heterodimer FokI

    Depositor

    Kosuke Yusa

    Insert

    ZFN targeting the AAVS1 locus
    

    Expression

    Mammalian

    Promoter

    CMV promoter

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. dCas9-CRY2Clust-FUSN

    Plasmid

    #200967

    Purpose

    induction of phase-separated condensate mediated by FUSN-IDR

    Depositor

    Jian Xu

    Article

    Kim et al Sci Adv. 2023 Mar 31;9(13):eadg1123. doi

    Insert

    FUSN
    

    Use

    Lentiviral

    Expression

    Bacterial and Mammalian

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. mEGFP-HRas

    Plasmid

    #18662

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. Rc/CMV cyclin A

    Plasmid

    #8959

    Depositor

    Bob Weinberg

    Article

    Hinds et al Cell. 1992 Sep 18. 70(6):993-1006.

    Insert

    cyclin A (CCNA2 Human)
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pST1374-NLS-flag-linker-Cas9

    Plasmid

    #44758

    Depositor

    Xingxu Huang

    Article

    Shen et al Cell Res. 2013 Apr 2. doi: 10.1038/cr.20

    Insert

    Human codon optimized Cas9
    

    Use

    CRISPR

    Tags

    32 amino acid linker, Flag, and NLS

    Expression

    Mammalian

    Mutation

    codon-optimized

    Promoter

    CMV

    Available Since

    April 24, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pmiRA1-MYB

    Plasmid

    #141120

    Purpose

    Lentiviral overexpression of MYB in mammalian cells.

    Depositor

    Jianjun Chen

    Article

    Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

    Insert

    MYB (MYB Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-dCas9-FLp300

    Plasmid

    #61356

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoter

    Depositor

    Charles Gersbach

    Article

    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

    Insert

    S.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Flag, HA, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    CMV

    Available Since

    April 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. PB-TAC-ERN

    Plasmid

    #80475

    Purpose

    All-in-One piggyBac transposon Destination vector for dox-inducible expression of Gateway cloned elements (inducible mCherry and constitutive rtTA and neomycin resistance) aka. KW111

    Depositor

    Knut Woltjen

    Article

    Kim et al Methods Mol Biol. 2016;1357:111-31. doi:

    Type

    Empty backbone

    Use

    Piggybac transposon

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. U6-BbsI-GGsite-mCherry-Puro

    Plasmid

    #221232

    Purpose

    Cloning vector for sgRNA with mCherry and Puro

    Depositor

    Fei Chen

    Article

    Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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