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HLA_DRB1_04_01

Plasmid

#165013

Purpose

Expression of HLA-DRB1*04:01 cDNA

Depositor

Matthew Meyerson

Publication

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*04:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-25xERSE2-minP-EGFP

Plasmid

#159667

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the ER stress response element-2 (ERSE2)

Depositor

Seiichi Oyadomari

Publication

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xERSE2-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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lenti-25xAARE-minP-EGFP

Plasmid

#159666

Purpose

EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

Depositor

Seiichi Oyadomari

Publication

Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

Insert

25xAARE-minP-EGFP

Use

Lentiviral

Expression

Mammalian

Available since

March 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX-IRES-Hygro_RGECO1.2

Plasmid

#164592

Purpose

Stable integration and expression of RGECO1.2 in mammalian cells

Depositor

Joseph Hyser

Publication

Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

Insert

R-GECO1.2

Use

Lentiviral

Expression

Mammalian

Mutation

R-GECO1 M164R/I166V/V174L/F222L/N267S/S270T/I330M…

Promoter

CMV

Available since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAV-Ef1a-DIO-PPO-Venus

Plasmid

#139505

Purpose

AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Publication

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Has service

AAV8 and AAV9

Insert

parapinopsin

Use

AAV

Tags

Venus tag following 3x Ala linker

Expression

Mammalian

Promoter

Ef1a

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTet-O-ATOH1-T2A-PuroR

Plasmid

#162342

Purpose

Lentiviral expression of ATOH1 under the control of the TetON promoter

Depositor

Charles Gersbach

Publication

Black et al Cell Rep. 2020 Dec 1;33(9):108460. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

T2A-PuroR

Expression

Mammalian

Promoter

Tet-ON

Available since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLVX-EF1a-PTDSS1-IRES-Puromycin

Plasmid

#134667

Purpose

Expresses EGFP-tagged Mitochondria-associated ER membrane marker

Depositor

David Andrews

Publication

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

PTDSS1 (PTDSS1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pLJC6-GLS-3xFLAG

Plasmid

#163450

Purpose

lentiviral overexpression vector for human GLS

Depositor

Jason Cantor

Publication

Rossiter et al Cell Metab. 2021 Feb 23. pii: S1550-4131

Insert

GLS (GLS Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

See: comments

Promoter

Ubc

Available since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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Lys-eMagB-iRFP

Plasmid

#162249

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

p18-eMagB-iRFP

Expression

Mammalian

Promoter

beta-actin promoter

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mCherry- eMagBF - 5ptaseOCRL

Plasmid

#162254

Purpose

Blue-light dependent heterodimerization system

Depositor

Pietro De Camilli

Publication

Benedetti et al Elife. 2020 Nov 11;9. pii: 63230. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry- eMagBF - 5ptaseOCRL

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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scFLARE1

Plasmid

#158738

Purpose

Mammalian expression of scFLARE1 component

Depositor

Alice Ting

Publication

Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

Insert

scFLARE1

Use

AAV

Tags

Flag, V5

Expression

Mammalian

Mutation

Truncated form of TEV protease (220-242 amino aci…

Promoter

CMV

Available since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Cas9-EGFP

Plasmid

#159126

Purpose

Expresses active Cas9 fused with EGFP for fluorescence microscopy

Depositor

Taekjip Ha

Publication

Liu et al Science. 2020 Jun 12;368(6496):1265-1269

Insert

Cas9 fused to EGFP

Use

CRISPR and Retroviral

Tags

EGFP

Expression

Mammalian

Promoter

MSCV LTR promoter

Available since

Dec. 14, 2020

Availability

Academic Institutions and Nonprofits only

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piGECI-N1

Plasmid

#160421

Purpose

Cytoplasmic expression of a near-infrared genetically encoded calcium indicator iGECI

Depositor

Vladislav Verkhusha

Publication

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iGECI

Expression

Mammalian

Promoter

CMV

Available since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pFUSE-rIgG-Fc2-ICAM1

Plasmid

#156462

Purpose

Expresses a rabbit Fc-ICAM1 fusion protein in mammalian cells

Depositor

Dirk Mielenz

Publication

Reimer et al Cell Rep. 2020 Aug 11;32(6):108030. doi:

Insert

Intercellular Adhesion Molecule 1 (Icam1 Mouse)

Tags

ICAM1-rFc

Expression

Bacterial and Mammalian

Mutation

endogenous signal peptide replaced with IL2 signa…

Promoter

hEF1-HTLC

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-gRNA

Plasmid

#158973

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA (gRNA3) expression plasmid.

Depositor

Charles P. Lai

Publication

Chien et al Nucleic Acids Res. 2020 Aug 14. pii: 589

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRNA3

Expression

Mammalian

Promoter

U6

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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Tol2-CAG::Palmbow

Plasmid

#158993

Purpose

Multicolor membrane-restricted labeling with a genome-integrating Tol2 transposon vector (randomly expressing palmitoylated mCherry, mEYFP or mCerulean upon Cre recombination)

Depositor

Jean Livet

Publication

Loulier et al Neuron. 2014 Feb 5;81(3):505-20. doi: 10

Insert

Palmbow

Tags

The default expressed EBFP2 is fused to H2B; oth…

Expression

Mammalian

Promoter

CAG

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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hHS1

Plasmid

#159169

Purpose

Human codon-optimized cytosolic labile heme reporter

Depositor

Amit Reddi

Publication

Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

hHS1

Expression

Mammalian

Promoter

CMV

Available since

Sept. 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pKS004 - pCAGGS-3XFLAG-CTCF-eGFP

Plasmid

#156438

Purpose

For transient expression of mouse CTCF tagged with eGFP

Depositor

Elphege Nora

Publication

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mouse CTCF (Ctcf Mouse)

Expression

Mammalian

Mutation

none

Available since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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mito-mScarlet

Plasmid

#141153

Purpose

Fluorescent labeling of the mitochondrial matrix in mammalian cells

Depositor

Gia Voeltz

Publication

Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

COX4 (COX4 Budding Yeast)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA5 CMV 3xFG eIF4G1

Plasmid

#158775

Purpose

pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

Depositor

Benjamin Blencowe

Publication

Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

Insert

eIF4G1

Tags

3xFlag

Expression

Mammalian

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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