78,292 results

1. pcDNA-iLACCO1.2

   Plasmid

   #218823

   Purpose

   Mammalian expression of genetically encoded green fluorescent L-lactate biosensor with high affinity

   Depositor

   Robert Campbell

   Article

   Hario et al ACS Cent Sci. 2024 Jan 31;10(2):402-416.

   Insert

   iLACCO1.2
   

   Expression

   Mammalian

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSCAR_Cas9-hygro_GFP

   Plasmid

   #162075

   Purpose

   3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

   Depositor

   Robert Manguso

   Article

   Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

   Inserts

   Cas9-2a-hygromycin B phosphotransferase
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Mutation

   codon-optimized for expression in M. musculus

   Promoter

   EF1a and sv40

   Available Since

   Feb. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Bsd

   Plasmid

   #214370

   Purpose

   Cloning vector with Blasticidin

   Depositor

   Bertrand Collet

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA Flag Lamp2b-HA

   Plasmid

   #71292

   Purpose

   Flag tag with 3 residue spacer fused to the N-terminus of Lamp2b, a transmembrane exosomal protein. 3 residue spacer then HA tag fused to the C-terminus of Lamp2b.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Tags

   Flag, HA, and Lamp2 signal peptide

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-EGFR-V5/HIS

   Plasmid

   #201102

   Purpose

   expression of human EGFR receptor tyrosine kinase in mammalian cells

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   human EGFR receptor tyrosine kinase, full length, wildtype (EGFR Human)
   

   Tags

   V5/His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-TrxRFP2

   Plasmid

   #172322

   Purpose

   an improved fluorescent biosensor for thioredoxin redox

   Depositor

   Huiwang Ai

   Article

   Pang et al Biochemistry. 2022 Mar 1;61(5):377-384.

   Insert

   TrxRFP2, an improved version from TrxRFP1
   

   Tags

   His-tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. mCh-KIF5B*-strep

   Plasmid

   #120164

   Purpose

   Expresses a chimera of mCherry (fluorescent tag), a KIF5B-based anterograde motor and streptavidin

   Depositor

   Juan Bonifacino

   Article

   Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

   Insert

   kinesin family member 5B (Kif5b Mouse)
   

   Tags

   Streptavidin and mCherry

   Expression

   Mammalian

   Mutation

   Truncated KIF5B: 1-555 aa

   Promoter

   CMV

   Available Since

   July 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. rtTA-N144

   Plasmid

   #66810

   Purpose

   2nd generation lentiviral transfer plasmid. Expresses the reverse tetracycline-controlled transactivator (rtTA) with hygromycin resistance

   Depositor

   Andrew Yoo

   Article

   Richner et al Nat Protoc. 2015 Oct;10(10):1543-55. doi

   Insert

   Reverse tetracycline-controlled transactivator
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   Human EF1a

   Available Since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-RLuc8.6-535

   Plasmid

   #87125

   Purpose

   Mammalian expression vector expressing red shifted variant of the reporter gene-Renilla luciferase (Rluc8.6)

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Nat Methods. 2007 Aug;4(8):641-3. Epub 2

   Insert

   Renilla Luciferase
   

   Expression

   Mammalian

   Mutation

   RLuc8/ A123S/D154M/E155G/D162E/I163L/V185L.

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. phDLX1-N174

    Plasmid

    #60859

    Purpose

    2nd generation lentiviral transfer plasmid. Expresses DLX1 under the EF1a promoter

    Depositor

    Andrew Yoo

    Article

    Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

    Insert

    DLX1 (DLX1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. SPLICS LY-MT Long P2A

    Plasmid

    #213613

    Purpose

    Detect the long-range Lysosome-Mitochondria contact

    Depositor

    Tito Calì

    Article

    Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

    Insert

    SPLICS LY-MT Long P2A
    

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2-MT GLI2 delta N

    Plasmid

    #17649

    Depositor

    Erich Roessler

    Article

    Roessler et al Hum Mol Genet. 2005 Aug 1. 14(15):2181-8

    Insert

    GLI2 (GLI2 Human)
    

    Tags

    6x Myc

    Expression

    Mammalian

    Mutation

    Missing 328 N-terminal amino acids

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRRL_SRSF2_WT_mCherry

    Plasmid

    #84020

    Purpose

    expression of SRSF2 in mammalian cells

    Depositor

    Robert Bradley

    Article

    Kim et al Cancer Cell. 2015 May 11;27(5):617-30. d

    Insert

    SRSF2 (SRSF2 Human)
    

    Use

    Lentiviral

    Tags

    2TA-mCherry and Flag

    Expression

    Mammalian

    Promoter

    PGK

    Available Since

    Oct. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 mCherry LIC cloning vector (6B)

    Plasmid

    #30125

    Purpose

    Empty LIC vector; adds an mCherry gene to the C-terminus of your protein of interest.

    Depositor

    Scott Gradia

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    June 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV.CAG.(mito).iATPSnFR2.A95A.A119L.miRFP670nano3

    Plasmid

    #209723

    Purpose

    Expresses mitochondrially targeted low affinity ratiometric ATP sensor

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).iATPSnFR2.A95A.A119L.miRFP670nano3
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUltra-U6-gRNAs1-5

    Plasmid

    #178569

    Purpose

    Constitutive expression of a gRNA-tRNA array for macsGESTALT editing

    Depositor

    Christopher Lengner

    Article

    Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.

    Insert

    5 gRNA-tRNA array with U6 promoter
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLenti6MFV SLC10A1

    Plasmid

    #194130

    Purpose

    Expresses NTCP (SLC10A1) protein in mammalian cells

    Depositor

    Jun Liu

    Insert

    SLC10A1 (SLC10A1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCK-mScarlet3_C1

    Plasmid

    #189771

    Purpose

    Construct for labelling plasma membranes in mammalian cells using LCK-mScarlet3

    Depositor

    Dorus Gadella

    Article

    Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

    Insert

    LCK-mScarlet3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. EBFP-C1

    Plasmid

    #54738

    Purpose

    Localization: C1 Cloning Vector, Excitation: 387, Emission: 450

    Depositor

    Michael Davidson

    Type

    Empty backbone

    Tags

    EBFP

    Expression

    Mammalian

    Available Since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Tags

    HIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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