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82,463 results

Showing: 1921 - 1940 of 82463 results

  14. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    107941-plasmid-map-sequence-id-418295

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  15. C1(1-29)-miniTurbo-V5_pCDNA3

    Plasmid
    #107174
    Purpose
    expresses V5-tagged miniTurbo on the mammalian ER membrane
    Depositor
    Alice Ting
    Publication
    Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniTurbo (BirA mutant)
    Expression
    Mammalian
    Mutation
    aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …
    Available since
    May 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    107174-plasmid-map-sequence-id-205307

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  16. pEGFP-N1 Full length Importin beta

    Plasmid
    #106941
    Purpose
    Transient expression of GFP-tagged Importin beta in mammalian cells (CMV promoter)
    Depositor
    Patrizia Lavia
    Publication
    Ciciarello et al J Cell Sci. 2004 Dec 15. 117(Pt 26):6511
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human Importin beta-1 (KPNB1) (KPNB1 Human)
    Tags
    GFP
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available since
    Feb. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    106941-plasmid-map-sequence-id-204312

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  18. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Publication
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    13 Articles
    92062-plasmid-map-sequence-id-180159

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Showing: 1921 - 1940 of 82463 results