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p35S:Citrine-TurboID-3xHA

Plasmid

#199243

Purpose

This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

Depositor

Savithramma Dinesh-Kumar

Article

Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

Insert

Citrine-TurboID

Tags

3xHA and TurboID-3xHA

Expression

Plant

Promoter

CaMV 35S

Available Since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUB-Cas9

Plasmid

#86556

Purpose

For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

Depositor

Andreas Weber

Article

Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

Insert

Cas9

Use

CRISPR

Expression

Plant

Mutation

Cas9 is codon optimized for C. reinhardtii

Promoter

UBIQUITIN10

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pB-CMPE-ePPEplus

Plasmid

#205246

Purpose

For plant prime editing in wheat plants

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

Csy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS

Use

CRISPR

Expression

Plant

Mutation

Detailed in manuscript

Available Since

May 12, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU6-HV5

Plasmid

#239470

Purpose

Encodes a gRNA expression cassette driven by the AtU6 promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U6-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-AtU3b-HV5

Plasmid

#239477

Purpose

Encodes a gRNA expression cassette driven by the AtU3b promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

U3b-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMD18T-At7SL-HV5

Plasmid

#239478

Purpose

Encodes a gRNA expression cassette driven by the At7SL promoter.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

7SL-gRNA

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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p1300-dCas9-HV5

Plasmid

#239479

Purpose

An intermediate vector is used to assemble three gRNAs via Golden Gate cloning.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

UBQ-dCAS9

Use

CRISPR

Expression

Plant

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMpGWB128

Plasmid

#68582

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB436

Plasmid

#68701

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pDicAID_nCas9-PmCDA_NptII_Della

Plasmid

#91694

Purpose

Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

PcUbi

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pS-cr:GFP

Plasmid

#196295

Purpose

35S promoter-driven expression of the positive-sense TSWV S RNA segment encoding crRNA:GFP fusion in place of the NSs

Depositor

Zhenghe Li

Article

Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

Insert

Full length TSWV S antigenome encoding crRNA:GFP fusion in place of viral NSs

Use

CRISPR

Tags

SpeI-DR-BsaI-DR-SpeI

Expression

Plant

Promoter

duplicated cauliflower mosaic virus 35S promoter

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUC57-CmYLCV

Plasmid

#205245

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

CmYLCV-csy4_binding_site-esgRNA-tevopreQ1-csy4_binding_site

Use

CRISPR

Expression

Plant

Promoter

CmYLCV

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDESTpK7WG-RPS5Ap-mito

Plasmid

#163145

Purpose

Plant mitoTALEN vector assembly, Destination vector for plants to express ORFs from entry clone with mitochondrial presequence under the RPS5A promoter

Depositor

Shin-ichi Arimura

Article

Arimura et al Plant J. 2020 Oct 24. doi: 10.1111/tpj.1

Insert

RPS5A promoter and mitochondrial presequence in front of attR1 in pk7WG

Expression

Plant

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pYPQ133B2.0

Plasmid

#99892

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pLCS99

Plasmid

#209327

Purpose

Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of untagged protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCBL101.1

Plasmid

#199724

Purpose

A pVS1-based T-DNA binary vector with NPTII as a plant selectable marker gene. BsaI site was removed.

Depositor

Kan Wang

Article

Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

Insert

Multiple Cloning Site, NPTII

Expression

Plant

Mutation

"GAGACC" to "GATACC"

Available Since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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EC67908

Plasmid

#211795

Purpose

Over expresses Cas12a and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Insert

HptII, Cas12a, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB660-CYCD3;1

Plasmid

#140751

Purpose

Inducing plant cells to enter mitosis

Depositor

Bo Liu

Article

Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

Insert

Cyclin D3;1 (CYCD3;1 Mustard Weed)

Tags

TagRFP

Expression

Plant

Promoter

CaMV 35 promoter

Available Since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB314

Plasmid

#68642

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWBs00

Plasmid

#222476

Purpose

Marchantia polymorpha transformation vector carrying sulfadiazine selection marker.

Depositor

Philip Carella

Article

Robinson et al J Exp Bot. 2024 Jun 2:erae256. doi: 10.1

Type

Empty backbone

Expression

Plant

Available Since

Sept. 9, 2024

Availability

Academic Institutions and Nonprofits only

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