4,240 results

1. pGWB552

   Plasmid

   #74882

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with G3GFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Oct. 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ134-STU-Lb

   Plasmid

   #138105

   Purpose

   Golden Gate entry vector for 4th crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYPQ133-STU-Lb

   Plasmid

   #138102

   Purpose

   Golden Gate entry vector for 3rd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pEG302 22aa SunTag VP64 gRNA4 (FWA)

   Plasmid

   #120249

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNA

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PROM5_MpEF1a

   Plasmid

   #136113

   Purpose

   Strong promoter from MpEF1a, that drives expression everywhere in Mp gemmae but stronger in the notches

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   PROM5_MpEF1a Mapoly0024s0116
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pDIRECT_23A

   Plasmid

   #91138

   Purpose

   Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:bar

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Engineering Reagent: 35S:AtCas9 + AtU6:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGT10446

   Plasmid

   #192353

   Purpose

   L0 MoClo-compatible GoldenGate-module of the 35S terminator (t35S) with stop

   Depositor

   Tom Schreiber

   Article

   Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

   Insert

   TTCG_Stop-t35S_CGCT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Nov. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTRANS_210

   Plasmid

   #91108

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   35S

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGW-TurboID-GOI-GFP

   Plasmid

   #209383

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQ132-STU-Lb

    Plasmid

    #138099

    Purpose

    Golden Gate entry vector for 2nd crRNA cloning with LbCas12a crRNA scaffold flanked by HH and HDV ribozymes

    Depositor

    Yiping Qi

    Article

    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKEE401

    Plasmid

    #91715

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Kan resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pYPQ166

    Plasmid

    #109328

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Lee et al Plant Biotechnol J. 2018 Jul 4. doi: 10.

    Insert

    zCas9
    

    Use

    CRISPR; Gateway compatible zcas9 entry clone

    Tags

    3x FLAG, NLS and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. L2_UBE2:mT-N7-CsA

    Plasmid

    #136133

    Purpose

    Plasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35S:HygR p5-MpUBE2:mTurq-N7
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-Tnos

    Plasmid

    #211840

    Purpose

    Plant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    LRR-NEL
    

    Use

    Synthetic Biology

    Tags

    HA and mCherry

    Expression

    Plant

    Mutation

    Promoter

    UBIQUITIN10

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYUU

    Plasmid

    #159751

    Purpose

    Ubiquitin promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter). Selection of transgenic seeds by YFP fluoresce.

    Depositor

    Wolfgang Lukowitz

    Article

    Angulo et al bioRxiv

    Insert

    gRNA scaffold
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pXEE401B

    Plasmid

    #91716

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis. Contains Cas9 and empty gRNA scaffold, Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHEE901

    Plasmid

    #91707

    Purpose

    CRISPR/Cas9-mediated base editing in Arabidopsis. Contains Cas9(D10A) and empty gRNA scaffold, HygR

    Depositor

    Qi-Jun Chen

    Article

    Chen et al Sci China Life Sci. 2017 Mar 16. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pNC-Cam1304-MCS

    Plasmid

    #193387

    Purpose

    Nimble cloning-compatible vector for plant ectopic expression

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L1_lacZgRNA-Ck3

    Plasmid

    #136136

    Purpose

    L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-MpU6:lacZgRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMpGWB127

    Plasmid

    #68581

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    MpEF1alpha promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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