4,240 results

1. HBT_pcoCASphi_version2

   Plasmid

   #197932

   Purpose

   Cloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminal

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Insert

   pcoCasphi-2
   

   Use

   CRISPR

   Tags

   2xNLS-2xFLAG

   Expression

   Plant

   Mutation

   Promoter

   35SPPDK promoter

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pENTR_AtU6p_BsaI_gRNA

   Plasmid

   #225987

   Purpose

   To make Gateway entry clone of CRISPR guide RNA under AtU6p

   Depositor

   Imran Siddiqi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. pSW648 - pREV>>

   Plasmid

   #115996

   Purpose

   destination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression

   Depositor

   Sebastien Wolf

   Article

   Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

   Insert

   pREV:GR-LhG4:tRBCS:F-H adapter-H-A adapter::pOp4:SP(ER)-mTurquoise2-HDEL:tUBQ10::SulfR
   

   Use

   Tags

   HDEL and SP(ER)

   Expression

   Plant

   Mutation

   Promoter

   Available since

   April 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. lvl0 sXVE

   Plasmid

   #153406

   Purpose

   Estradiol inducible system trans activator

   Depositor

   Naomi Nakayama

   Article

   Andreou et al bioRxiv, March 31, 2021

   Insert

   sXVE CDS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDGB3 alpha2 P35S:MS2:TV:Tnos (GB2048)

   Plasmid

   #160627

   Purpose

   TU for the constitutive expression of Ms2 fused to TV (TALx6 - VP128) activation domains

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35s-MS2:TV-Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGW-GFP-GOI-TurboID

   Plasmid

   #209382

   Purpose

   Gateway compatible plasmid for TurboID-based proximity labelling experiment in planta

   Depositor

   Rosa Lozano-Durán

   Article

   Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   TurboID gene sequence C249A

   Promoter

   Available since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDGB3 alpha2 P35S:MS2:VPR:Tnos (GB1830)

   Plasmid

   #160624

   Purpose

   TU for the constitutive expression of the MS2 coat protein fused on Ct to the activation domain VPR (VP64+p65+Rta)

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   P35S:MS2:VPR:Tnos
   

   Use

   Tags

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   P35S

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCHW00016

   Plasmid

   #222447

   Purpose

   Level 0, C terminal tag module (TTCG-GCTT) containing p65AD-NLS.

   Depositor

   Chih-Hang Wu

   Article

   Chiang et al bioRxiv 2024.03.23.586378

   Insert

   p65AD-NLS
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   no

   Promoter

   Available since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. PEP101E

   Plasmid

   #97387

   Purpose

   binary construct of cytosolic sfGFP1-10 for plant

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

   Insert

   sfGFP1-10OPT
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAGT5439

    Plasmid

    #192351

    Purpose

    L0 MoClo-compatible GoldenGate-module of the OCS terminator (tOCS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tOCS_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAGT9403

    Plasmid

    #192352

    Purpose

    L0 MoClo-compatible GoldenGate-module of the NOS terminator (tNOS) with stop

    Depositor

    Tom Schreiber

    Article

    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4

    Insert

    TTCG_Stop-tNOS_CGCT
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAK-EL-03

    Plasmid

    #125762

    Purpose

    Endlinker for tRNA-gRNA system when using only three guides

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    Endlinker
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDW1775

    Plasmid

    #13456

    Depositor

    Daniel Voytas

    Article

    Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    AcV5

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFH54

    Plasmid

    #128183

    Purpose

    Level1 AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    AtUBI10p::SpCas9 (Arabidopsis codon optimized):pea3At
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pZG61

    Plasmid

    #202575

    Purpose

    RNA editing in plants. Plasmid contains 35S-dADARcd-LIC1-ccdB-LIC2-35S-hptII

    Depositor

    Guoyong Xu

    Article

    Zhou et al Cell Discov. 2021 Aug 24;7(1):72. doi: 1

    Insert

    HyperADARcd
    

    Use

    Tags

    Expression

    Plant

    Mutation

    E488Q

    Promoter

    Available since

    Dec. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PROM_MpUBE2

    Plasmid

    #136109

    Purpose

    PROM for cloning with 5UTR_MpUBE2. PROM+5UTR_MpUBE2 has ubiquitous expression in early satges of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    PROM_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. 5UTR_MpUBE2

    Plasmid

    #136110

    Purpose

    5UTR for cloning with PROM_MpUEB2. PROM+5UTR_MpUBE2 has ubiquitous expression in early stages of gemmae development.

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    5UTR_MpUBE2 Mapoly0106s0019
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pCk2_spacer

    Plasmid

    #136072

    Purpose

    Use when making even level constructs (L2,...) if pCk2 position (position 2) has no construct assigned

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    Odd spacer
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Osa-pCAMBIA-1301-STTM319

    Plasmid

    #84201

    Purpose

    Disturb the expression of miR319 in rice

    Depositor

    Guiliang Tang

    Article

    Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

    Insert

    miR319
    

    Use

    Tags

    Expression

    Bacterial, Plant, and Yeast…

    Mutation

    Promoter

    2x35S

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEE393

    Plasmid

    #149283

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only