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pPSup_35Senh(fwd)_35Spr+synJ

Plasmid

#149422

Purpose

Plasmid for STARR-seq in tobacco leaves. The GFP reporter gene is under the control of a 35S minimal promoter and an upstream 35S core enhancer.

Depositor

Stan Fields

Article

Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

Insert

35S core enhancer

Expression

Plant

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pYL322d1/N-eGFP

Plasmid

#183151

Purpose

Plant transient expression vector containing eGFP tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of eGFP.

Depositor

Yao-Guang Liu, Qinlong Zhu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Type

Empty backbone

Expression

Plant

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pEG302-SunTag-FWAgRNA-4-22aa-TET1cd

Plasmid

#115344

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN422aa_OCS

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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PEP108E

Plasmid

#97394

Purpose

binary construct of plasma membrane-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Tags

Plasma membrane target

Expression

Plant

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pGEL852

Plasmid

#214252

Purpose

Expression TadCBEd_V106W in rice cells

Depositor

Yong Zhang

Article

Fan et al Nat Commun. 2024 Jun 14;15(1):5103. doi:

Type

Empty backbone

Expression

Plant

Available Since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS_260d

Plasmid

#91126

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

PvUbi2

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCk4

Plasmid

#136698

Purpose

Acceptor plasmid for odd level position 4, lacZ blue-white screening.

Depositor

Nicola Patron

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pMpGWB426

Plasmid

#68691

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

No Promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB602-TP-mCherry-UnaG

Plasmid

#203483

Purpose

For Agrobacterium transformation. A plastid transit peptide fused mCherry-UnaG under the control of the cauliflower mosaic virus (CaMV) 35S promoter.

Depositor

Yutaka Kodama

Article

Ishikawa et al Sci Adv. 2023 Jun 9;9(23):eadh4787. doi:

Insert

Plastid transit peptide fused mCherry-UnaG

Expression

Bacterial and Plant

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB116

Plasmid

#68570

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Nuclear localization signal-tagged tdTomato (tdTo…

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pYPQ284-RVRR

Plasmid

#160327

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized Mb2Cas12a with N563R, K569V, N573R and K625R mutations, without promoter

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

Mb2Cas12a RVRR variant

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Mutation

N563R, K569V, N573R and K625R

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pFBP_promoter_acceptor

Plasmid

#189920

Purpose

Binary vector to accept a custom promoter sequence to drive fungal luciferase expression by Agrobacterium-mediated transient transformation

Depositor

Adam Steinbrenner

Article

Garcia et al Mol Plant Microbe Interact. 2023 Mar;36(

Type

Empty backbone

Expression

Plant

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGWB326

Plasmid

#68654

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

TagRFP

Expression

Plant

Promoter

No Promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pFGC5941-PacI-ath-STTM165/166-48nt

Plasmid

#84091

Purpose

target miRNA165/166 for destruction in Arabidopsis thaliana

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Insert

STTM165/166

Expression

Plant

Promoter

2x35S

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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p32Lb

Plasmid

#158154

Purpose

Construct conventional Cpf1 vectors that uses U3p and UBI10p to express single crRNA (or PTC/CA) and FnCpf1, respectively. For plant genome editing.

Depositor

Kabin Xie

Article

Ding et al Mol Plant. 2018 Apr 2;11(4):542-552. doi

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pICSL30003

Plasmid

#50304

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, no stop codon, mCherry variant of RFP (Discosoma sp.)

Expression

Plant

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMU-TGN41r

Plasmid

#61176

Purpose

Fluorescent reporter for trans-Golgi network mCherry-MtSYP41 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

MtSYP41

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

March 30, 2015

Availability

Academic Institutions and Nonprofits only

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pGWB519

Plasmid

#74861

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_211

Plasmid

#91110

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: BeYDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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