4,240 results

1. pC1300_pUB10_pcoCASphi_E9t_version2_U6_AtPDS3_gRNA10

   Plasmid

   #197952

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter, with SV40 NLS at the C-terminal , and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. BCJJ345

   Plasmid

   #117504

   Purpose

   YAO:Cas9:E9 Golden Gate Level 1 Position 2 reverse

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:tagt:YAO:Cas9-3(intron):E9:ttgc:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtYAO

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMpGWB301

   Plasmid

   #68629

   Purpose

   Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

   Depositor

   Takayuki Kohchi

   Article

   Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   June 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. UBQ10:sXVE:GFP1–9

   Plasmid

   #108187

   Purpose

   Binary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plants

   Depositor

   Tzu-Yin Liu

   Article

   Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

   Insert

   GFP1-9
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBQ10:sXVE:

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. barII-UT-ABAleon2.1

   Plasmid

   #106981

   Purpose

   ABAleon2.1 in plant expression vector (Basta resistance)

   Depositor

   Julian Schroeder

   Article

   Waadt et al Elife. 2014 Apr 15;3:e01739. doi: 10.755

   Insert

   ABAleon2.1
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   UBQ10

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFRETtv-2in1-NC

   Plasmid

   #105122

   Purpose

   FRET/FLIM analysis in plants (stable or transient transformation)

   Depositor

   Christopher Grefen

   Article

   Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

   Type

   Empty backbone

   Use

   Tags

   mTRQ2 and mVenus

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Feb. 22, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. L1_KanR-Ck1

   Plasmid

   #136123

   Purpose

   L1 in position 1 (next to LB in L2 construct), for KanR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-MpEF1a:nptII_3t35S
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

8. pCk1_spacer

   Plasmid

   #136071

   Purpose

   Use when making even level constructs (L2,...) if pCk1 position (position 1) has no construct assigned

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   Odd spacer
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pYPQ281 (MbCas12a)

   Plasmid

   #138113

   Purpose

   Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized MbCas12a without promoter

   Depositor

   Yiping Qi

   Article

   Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

   Insert

   MbCas12a
   

   Use

   CRISPR; Gateway compatible cas12a entry clone

   Tags

   NLS

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSC6

    Plasmid

    #104780

    Purpose

    pSC218UG-At Ubiquitin10:Gateway expression cassette binary vector

    Depositor

    Robert Stupar

    Article

    Curtin et al Plant Biotechnol J. 2017 Oct 31. doi: 10

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Jan. 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGWB508

    Plasmid

    #74850

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Oct. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMpGWB405

    Plasmid

    #68670

    Purpose

    Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

    Depositor

    Takayuki Kohchi

    Article

    Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

    Type

    Empty backbone

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus 35S promoter

    Available since

    June 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. lvl0 alcSynth-34S

    Plasmid

    #153411

    Purpose

    Ethanol system inducible promoter

    Depositor

    Naomi Nakayama

    Article

    Andreou et al bioRxiv, March 31, 2021

    Insert

    alcSynth-34S promoter
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pYPQ141A2.0

    Plasmid

    #99896

    Purpose

    Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU6 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTRANS_222

    Plasmid

    #91116

    Purpose

    Transformation, Type: T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: ToLCV

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ141-ZmUbi-RZ-Fn

    Plasmid

    #108864

    Purpose

    FnCpf1 Gateway crRNA entry plasmid using Zea mays Ubi promoter and double ribozyme processing

    Depositor

    Yiping Qi

    Article

    Zhong et al Mol Plant. 2018 Jul 2;11(7):999-1002. do

    Type

    Empty backbone

    Use

    CRISPR; Gateway compatible crrna entry clone

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ230-RRVQ

    Plasmid

    #200188

    Purpose

    Gateway entry clone (attL1 & attR5) for LbCas12a-RRVQ mediated genome editing

    Depositor

    Yiping Qi

    Article

    Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

    Insert

    LbCas12a-RRVQ
    

    Use

    CRISPR; Gateway compatible lbcas12a-rrv entry clo…

    Tags

    NLS

    Expression

    Plant

    Mutation

    G146R, D156R, R182V, E795Q

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGMV-U

    Plasmid

    #112797

    Purpose

    Universal BeYDV-derived vector for cloning of up to four gRNAs

    Depositor

    Humberto Prieto

    Article

    Prieto et al In: Cantu D., Walker M. (eds) The Grape

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMpGWB103-Citrine

    Plasmid

    #79137

    Purpose

    Binary vectors for expression of Citrine fluorescent protein in M. polymorpha

    Depositor

    Yutaka Kodama

    Article

    Tsuboyama et al Plant Cell Physiol. 2014 Jan;55(1):229-3

    Insert

    Citrine
    

    Use

    Tags

    Citrine

    Expression

    Plant

    Mutation

    Promoter

    MpEF1a promoter

    Available since

    Aug. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pFRETgc-2in1-NC

    Plasmid

    #105121

    Purpose

    FRET/FLIM analysis in plants (stable or transient transformation)

    Depositor

    Christopher Grefen

    Article

    Hecker et al Plant Physiol. 2015 Jul;168(3):776-87. d

    Type

    Empty backbone

    Use

    Tags

    mCherry and mEGFP

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge