4,552 results
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Plasmid#136138PurposePlasmid to accept gRNA target with SapI, lacZ blue-white screening. Contains Cas9 and HygR.DepositorInsertp5-35Sx2:HygR p5-MpU6:lacZgRNA p5-MpEF1a:Cas9-NLS
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pUC19/mChe-Px
Plasmid#183170PurposeConstruct for transient expression of peroxisomes-targeted mCherry in plants.DepositorInsertmCherry-Px
Tags10 aa peroxisome targeting signal 1 (IHHPRELSRL) …ExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT11
Plasmid#223383PurposeT-DNA vector for SpRY mediated mutagenesis for monocot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-OsU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT60
Plasmid#223432PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.DepositorInsert2x35s-dSpCas9-Act3.0-OsU3-gRNA scaffold 2.0
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceJune 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT61
Plasmid#223433PurposeT-DNA vector for dSpCas9 mediated gene activation for monocot plants; NGG PAM; dSpCas9-Act3.0 was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1 promoter; Hygromycin for plants selection.DepositorInsertZmUbi-dSpCas9-Act3.0-ZmUbi-gRNA scaffold 2.0-tRNA
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYPQAT08
Plasmid#223380PurposeT-DNA vector for SpRY mediated mutagenesis for dicot plants; NG or NA PAM preference; SpRY was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.DepositorInsertZmUbi-SpRY-AtU3-gRNA scaffold
UseCRISPRTags3x FLAG and NLSExpressionPlantAvailable SinceApril 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pECO200
Plasmid#169765PurposeExpress SpCas9 and Basta resistance geneDepositorTypeEmpty backboneUsePlant binary vectorExpressionPlantPromoter35S, AtU6Available SinceJuly 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGWB2-CYCD3;1
Plasmid#140750PurposeInducing plant cells to enter mitosisDepositorAvailable SinceMay 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyExpressionPlantPromoterUBQ10:sXVE:Available SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICSL1P1_AtU626Ter26
Plasmid#191769PurposeCas9 guide expression in dicotsDepositorInsertSpCas9 guide scaffold with AtU626 promoter/terminator
UseCRISPRExpressionPlantAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTRANS_232
Plasmid#91121PurposeTransformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: ToLCVDepositorTypeEmpty backboneExpressionPlantPromoter35SAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGB 35s:dCas:BRD:tNos (GB1172)
Plasmid#75401PurposeTranscriptional unit of (human codon optimized) inactivated Cas9 fused to the BRD Transcriptional RepressorDepositorInsertdCas9:BRD
UseCRISPR and Synthetic BiologyExpressionPlantPromoter35SAvailable SinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230-D156R (ttLbCas12a)
Plasmid#195366PurposeA temperature-tolerant LbCas12a mutant (D156R), also known as ttLbCas12aDepositorInsertLbCas12a-D156R (ttLbCas12a)
UseCRISPR; Gateway compatible lbcas12a-d156r (ttlbca…ExpressionPlantPromoterZmUBI1Available SinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
L1_UBE2:mS-Lt-Ck3
Plasmid#136131PurposeL1 in position 3, mark cell outline with mScarletIDepositorInsertp5-MpUBE2:mScarletI-Lti6b_3tNos-35S
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMK-N. tabacum atpB/accD
Plasmid#123582PurposeMoChlo kitDepositorInsertN. tabacum atpB/accD integration site
UseChloroplast transformation vectorExpressionPlantAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDGB3 alpha2 P35S:dCas9:SRDX:Tnos (GB1188)
Plasmid#160608PurposeTU for the expression of the dCas9 with the repressor domain SRDX as a CT fusion (CRISPR tools)DepositorInsertP35s:dCas9:SRDX:tNOS
UseSynthetic BiologyExpressionPlantMutationBsaI and BsmBI sites removedPromoterP35SAvailable SinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXYB089
Plasmid#239628PurposePlant RNA Vision constructs with no gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01
stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyExpressionPlantAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pXYB082
Plasmid#239578PurposePlant RNA Vision constructs with 41 bp gRNAs together with RFP inputDepositorInsertssfGFPn_Y66-P1_loop_01-stem01-gRNA01
gRNA02-stem_02-P1_loop_03-internal guide sequence-Ribozyme-sfGFPn_Y66
RFP
UseSynthetic BiologyExpressionPlantAvailable SinceJune 25, 2025AvailabilityAcademic Institutions and Nonprofits only