1,755 results

1. pPD96_32

   Plasmid

   #1504

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   IVSA, ATG-MitLS, gfpN, IVSR, gfpS65C, IVSS, IVST,…

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSEM255 - sgRNA mosTI Pmlc-2_GFP

   Plasmid

   #159835

   Purpose

   Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Insert

   sgRNA mosTI Pmlc-2_GFP
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDSP16 (5’Δ Cbr-unc-119(+) + loxN + MCS + 5’Δ mNeonGreen)

   Plasmid

   #193854

   Purpose

   Promoterless insertion vector for split Cbr-unc-19(+)/mNeonGreen landing pad

   Depositor

   Patrick Phillips

   Article

   Stevenson et al Elife. 2023 Jul 4;12:RP84831. doi: 10.75

   Insert

   5' Cbr-unc-119(+) + 5' mNeonGreen
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Cbr-unc-119p

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPD49_83

   Plasmid

   #1448

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   hsp16-25', hsp16-415', IVSA, rf2128

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFS16 Pmyo-3 NPP-9 mChBioFLAG

   Plasmid

   #45099

   Depositor

   Steven Henikoff

   Article

   Steiner et al Genome Res. 2012 Apr;22(4):766-77. doi:

   Inserts

   npp-9 (npp-9 Nematode)
   unc-119 (unc-119 Nematode)
   

   Use

   Tags

   BLRP, FLAG, and mCherry

   Expression

   Worm

   Mutation

   Promoter

   myo-3 and unc-119

   Available since

   June 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOL2339

   Plasmid

   #37839

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Ub-V
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   Promoter

   nhx-2

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pOL2340

   Plasmid

   #37841

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Ub-M
   

   Use

   Tags

   mCherry

   Expression

   Worm

   Mutation

   R77M

   Promoter

   nhx-2

   Available since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ2092 - [1-2] ENTR - Fluor - mNeon(PATC)

   Plasmid

   #159853

   Purpose

   Three-fragment Gateway compatible flourophore for expression in C. elegans

   Depositor

   Christian Froekjaer-Jensen

   Article

   Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

   Insert

   [1-2] ENTR - Fluor - mNeon(PATC)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Not applicable

   Promoter

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Prab-3::NLS::GCaMP6s

    Plasmid

    #68119

    Purpose

    Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

    Depositor

    Andrew Leifer

    Article

    Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

    Insert

    rab-3::NLS::GCaMP6s
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pPD95_77

    Plasmid

    #1495

    Depositor

    Andrew Fire

    Type

    Empty backbone

    Use

    Tags

    IVSA, rf2204, gfpN, IVSR, gfpS65C, IVSS, IVST, gf…

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSF11-wNIR-GECO2-T2A-HO1

    Plasmid

    #159606

    Purpose

    Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

    Depositor

    Robert Campbell

    Article

    Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

    Insert

    wNIR-GECO2-T2A-HO1
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    tag-168

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSEM388 - mosTI unc-119 - NNN - mScarlet - tbb-2

    Plasmid

    #182348

    Purpose

    mScarlet fusion cloning vector for mosTI(unc-119). Transgene inserted upstream of mScarlet and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDD400

    Plasmid

    #137024

    Purpose

    SapTrap mNG-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized mNeonGreen
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAS3-rab-3P::AI::ChR2(H134R)

    Plasmid

    #232601

    Purpose

    Pan-neuronal expression of opsin 'ChR2(H134R)', regulated by rab-3 promoter and unc-54 3' UTR

    Depositor

    Andrew Leifer

    Article

    Sharma et al Genetics. 2024 Jul 8;227(3):iyae077. doi

    Insert

    Channelrhodopsin2
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDD362

    Plasmid

    #137023

    Purpose

    SapTrap HaloTag-GLO donor

    Depositor

    Bob Goldstein, Daniel Dickinson

    Insert

    C. elegans GLO-optimized Halo Tag
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized Halo Tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAS1-rab3P::his-24::mNeptune

    Plasmid

    #124350

    Purpose

    Nuclear Localized Pan-Neuronal mNeptune

    Depositor

    Andrew Leifer

    Insert

    rab-3, mNeptune (rab-3 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHKMC1: Empty sgRNA for Cloning

    Plasmid

    #67720

    Purpose

    Empty vector for cloning sgRNA. NotI and BamHI for cloning sgRNA. PU6 driven sgRNA.

    Depositor

    Monica Colaiacovo

    Article

    Norris et al Genetics. 2015 Oct;201(2):449-58. doi: 1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    PU6

    Available since

    July 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge