1,737 results

1. Prig-3::CD4::spGFP1-10

   Plasmid

   #65829

   Purpose

   For membrane contact

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
   

   Use

   Tags

   spGFP1-10

   Expression

   Worm

   Mutation

   Promoter

   Prig-3

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. Prig-3::CD4::spGFP11

   Plasmid

   #65830

   Purpose

   For membrane contact

   Depositor

   Kang Shen, Cori Bargmann

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)
   

   Use

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   Promoter

   Prig-3

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TetR in pENTR1A (559-1)

   Plasmid

   #22265

   Depositor

   Eric Campeau

   Insert

   Tetracycline repressor (tetR )
   

   Use

   Tags

   Expression

   Insect, Mammalian, Worm Exp…

   Mutation

   Promoter

   Available since

   Dec. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKS2301

   Plasmid

   #37834

   Depositor

   Gary Silverman

   Article

   Miedel et al PLoS One. 2012;7(7):e40145. Epub 2012 Ju

   Insert

   Pnhx-2cpl-1::YFP (cpl-1 Nematode)
   

   Use

   Tags

   YFP

   Expression

   Worm

   Mutation

   Promoter

   nhx-2

   Available since

   Dec. 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. Prab-3::NLS::GCaMP6s

   Plasmid

   #68119

   Purpose

   Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

   Depositor

   Andrew Leifer

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

   Insert

   rab-3::NLS::GCaMP6s
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   rab-3

   Available since

   Aug. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJS145

   Plasmid

   #179159

   Purpose

   Encodes 2xNLS::GFP(flexon), codon optimized for C. elegans. Blocks expression from ubiquitous promoter rps-27p with Flexon. Excision of flexon with Cre allows for strong expression.

   Depositor

   Iva Greenwald

   Article

   Shaffer et al Proc Natl Acad Sci U S A. 2022 Jan 18;11

   Insert

   rps-27p::2xNLS::GFP(flexon)::unc-54 3' UTR
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Flexon replacing 2nd intron of 2xNLS::GFP

   Promoter

   rps-27p

   Available since

   Jan. 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD283

   Plasmid

   #66824

   Purpose

   YPET^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   YPET-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized YPET

   Expression

   Worm

   Mutation

   Promoter

   Available since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPD49_78

   Plasmid

   #1447

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   hsp16-415', hsp16-25', IVSA, rf2128

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDD317 (Pmyo-2::GFP + SEC)

   Plasmid

   #73344

   Purpose

   SEC vector carry Pmyo-2::GFP and ccdB sites for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Hibshman et al BMC Biol. 2021 Dec 14;19(1):263. doi: 10

   Insert

   Pmyo-2::GFP + SEC
   

   Use

   CRISPR and Cre/Lox

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   myo-2

   Available since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCZGY3196

    Plasmid

    #172696

    Purpose

    Prgef-1-gfp1-10 Expresses split GFP parts 1-10 in all C. elegans neurons (only shows active fluorescence when binds to GFP11)

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    Prgef-1
    

    Use

    Tags

    GFP 1-10

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMLS134

    Plasmid

    #73714

    Purpose

    SapTrap destination vector for building sgRNA expression vectors.

    Depositor

    Erik Jorgensen

    Article

    Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

    Insert

    pU6::sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    SapI insertion site

    Promoter

    C. elegans U6 snRNA pol III promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. TC358

    Plasmid

    #122613

    Purpose

    TRE-delta-pes-10-GFP

    Depositor

    Tian Chi

    Article

    Mao et al iScience. 2019 Jan 25;11:224-237. doi: 1

    Insert

    7XTRE-delta-pes-10-GFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJH4542

    Plasmid

    #200779

    Purpose

    Ptwk-40s GCaMP6s T2A NLS::mNeptune unc-54 3' UTR C.elegans AVA,AVB and other neurons expression of GCaMP6 and nuclear mNeptune

    Depositor

    Mei Zhen

    Article

    Meng et al Sci Adv. 2024 Apr 12;10(15):eadk0002. do

    Insert

    GCaMP6
    

    Use

    Tags

    mNeptune

    Expression

    Worm

    Mutation

    Promoter

    Ptwk-40

    Available since

    May 7, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pGM32DEST

    Plasmid

    #74747

    Purpose

    Encodes for the QF-GR recombinant gene, splice linker, and mCherry reporter gene

    Depositor

    Jordan Ward, Keith Yamamoto

    Article

    Monsalve et al Genetics. 2019 Feb;211(2):419-430. doi:

    Inserts

    Gateway cassette
    QF-GR (NR3C1 Synthetic, Neurospora crassa, Human)
    

    Use

    Tags

    Fusion of the Neurospora crassa QF activator to t…

    Expression

    Worm

    Mutation

    Promoter

    No promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDYH5

    Plasmid

    #52032

    Purpose

    Expression of GFP-tagged DAF-16 in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Tao et al Elife. 2013;2:e00518. doi: 10.7554/eLife

    Insert

    DAF-16 (daf-16 Nematode)
    

    Use

    Tags

    GFP and HIS

    Expression

    Worm

    Mutation

    Promoter

    Available since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

    Plasmid

    #159803

    Purpose

    Expression construct with ubiquitous expression to test fluorescence level on microscope

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHW522 (Prab-3::cGAL-C::let-858 3'UTR)

    Plasmid

    #107130

    Purpose

    cGAL-C driver under the control of C. elegans rab-3 promoter

    Depositor

    Paul Sternberg

    Article

    Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

    Insert

    NLS::gp41-1-C-intein::cGAL(AD)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    C. elegans rab-3 promoter

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCFJ150-GFP(dpiRNA)::CDK-1

    Plasmid

    #107938

    Purpose

    cdk-1p::GFP(dpiRNA)::cdk-1::cdk-1 3'UTR construct used for MosSCI in nematode. GFP is optimized by removing all piRNA targeting sites to allow germline expression.

    Depositor

    Heng-Chi Lee

    Article

    Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

    Insert

    GFP(dpiRNA)::CDK-1 (cdk-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    cdk-1

    Available since

    April 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pXF88

    Plasmid

    #139039

    Purpose

    MosSCI SapTrap assembly: Cassette 3 for 4-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    halotag (includes STOP codon, PATC introns)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD122 (Peft-3::Cas9 + ttTi5605 sgRNA)

    Plasmid

    #47550

    Purpose

    Cas9 + sgRNA plasmid that is targeted to a genomic site near the ttTi5605 Mos1 insertion allele

    Depositor

    Bob Goldstein

    Article

    Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

    Inserts

    Cas9
    ttTi5605 sgRNA
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Promoter

    U6 and eef-1A.1 (eft-3)

    Available since

    Sept. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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