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pCFJ1985 - [1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Plasmid

#159846

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - TagBFP2(PATC, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2249 - [1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Plasmid

#159847

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - ce-GFP (1 intron, syntrons(3), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2370 - intron GG3 - 250bp

Plasmid

#159879

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

intron GG3 - 250bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCFJ2259 - Intron GG2 - 900 bp

Plasmid

#159881

Purpose

Golden Gate compatible intron with PATCs for reduced germline silencing

Depositor

Christian Froekjaer-Jensen

Publication

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

Intron GG2 - 900 bp

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSEM253 - sgRNA mosTI hygroR

Plasmid

#159827

Purpose

Single copy or array insertion by MosTI using split hygroR selection

Depositor

Christian Froekjaer-Jensen

Publication

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

sgRNA mosTI hygroR

Expression

Worm

Mutation

Not applicable

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pAS18

Plasmid

#158787

Purpose

Ribosome-tagged GCaMP (soma localized) expression pan-neuronally C. elegans

Depositor

Jennifer Garrison, Zachary Knight

Publication

Chen et al Neuron. 2020 Jun 5. pii: S0896-6273(20)3

Insert

Ptag-168::GCaMP6m::rpl-1a

Expression

Worm

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1347

Plasmid

#154310

Purpose

Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa Linker for CT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1838

Plasmid

#154324

Purpose

sgRNA Destination Vector for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SapTrap sgRNA (F+E) vector, K09B11.2 U6 promoter and 3'UTR

Use

CRISPR

Expression

Worm

Mutation

F+E mutations in sgRNA

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1659

Plasmid

#154314

Purpose

NT Donor-cassette for SapTrap

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

30aa linker::AID*::3xFLAG for NT slot

Use

CRISPR

Expression

Worm

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1836

Plasmid

#154337

Purpose

Promoter reporter

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

SV40 NLS::mScarlet_I::PEST (dpi)::-tbb-2 3'UTR

Expression

Worm

Mutation

Silent mutations to remove piRNA sites

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pJW1592

Plasmid

#121059

Purpose

GFP^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning homology arms.

Depositor

Jordan Ward

Publication

Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

Insert

GFP^SEC^BioTag::AID*::3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFLAG, Biotin acceptor peptide (BioTag), C. eleg…

Expression

Worm

Available since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pSDH52

Plasmid

#139049

Purpose

MosSCI SapTrap assembly: Cassette 3B for 5-cassette system

Depositor

Erik Griffin

Publication

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

epdz (includes STOP codon)

Expression

Worm

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pBN449

Plasmid

#129555

Purpose

GAL4-induced expression of codon-optimised FLP recombinase with unfused mNeongreen (mNG) as a marker in C. elegans

Depositor

Peter Askjaer

Publication

Ayuso et al MicroPubl Biol. 2019 Jan 29;2019:10.1791

Insert

15xUAS-∆pes-10-Flp-SL2

Tags

mNeonGreen

Expression

Worm

Promoter

UAS-∆pes-10

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSH87

Plasmid

#127705

Purpose

Pflp-13::gfp1-10

Depositor

David M. Miller

Publication

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

flp-13

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSH88

Plasmid

#127703

Purpose

Pacr-2::gfp1-10

Depositor

David M. Miller

Publication

He et al Genetics. 2019 Apr 5. pii: genetics.119.

Insert

GFP1-10

Expression

Worm

Promoter

acr-2

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab-3P::his-24::CyOFP

Plasmid

#124347

Purpose

Nuclear Localized Pan-Neuronal CyOFP

Depositor

Andrew Leifer

Insert

rab-3, CyOFP (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pAS1-rab3P::his-24::mNeptune

Plasmid

#124350

Purpose

Nuclear Localized Pan-Neuronal mNeptune

Depositor

Andrew Leifer

Insert

rab-3, mNeptune (rab-3 Nematode)

Expression

Worm

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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BSP607

Plasmid

#122252

Purpose

expression vector for mKO2 under EFT3 promoter

Depositor

Alexander Mendenhall

Publication

Sands et al Transl Med Aging. 2018 Jan;2:1-10. doi:

Insert

mKO2

Expression

Worm

Promoter

EFT3

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pPD_Pnpr-4::G-CaMP6s

Plasmid

#117423

Purpose

For Calcium imaging, expression of G-CaMP6 under the expression of a npr-4 promoter (AVA and RIV interneurons)

Depositor

Shohei Mitani

Publication

Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

Insert

G-CaMP6s

Expression

Worm

Promoter

npr-4

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMLS260

Plasmid

#73685

Purpose

Punc-17::2xNLS-FLP-D5 Expression vector

Depositor

Erik Jorgensen

Publication

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

Punc-17::2xNLS-FLP-D5::let-858 3'-UTR

Use

Flp/frt

Expression

Worm

Mutation

G5D

Promoter

Punc-17

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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