1,743 results

1. pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

   Plasmid

   #71720

   Purpose

   Expressing an mRuby-tagged TIR1 in the soma of C. elegans

   Depositor

   Abby Dernburg

   Article

   Zhang et al Development. 2015 Nov 9. pii: dev.129635

   Insert

   TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)
   

   Use

   Tags

   mRuby

   Expression

   Worm

   Mutation

   codon-optimized for expression in C. elegans; Two…

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFJ1415

   Plasmid

   #84828

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-1 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPD95_25

   Plasmid

   #1486

   Depositor

   Andrew Fire

   Type

   Empty backbone

   Use

   Tags

   IVSA, ATG-SecSig, pes10

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSEM238

   Plasmid

   #161515

   Purpose

   Neuronal expression of histamine-sensitive chloride channel for negative selection

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al MicroPubl Biol. 2021 Jan 8;2021. doi: 10

   Insert

   pEXP[Psnt-1 | histamine | rpl-3 UTR]
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Psnt-1

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. BiP 2: C. elegans BiP (hsp-4)-GFP

   Plasmid

   #21896

   Depositor

   David Ron

   Article

   Calfon et al Nature. 2002 Jan 3. 415(6867):92-6.

   Insert

   BiP (hsp-4 Nematode)
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Fusion between the GFP and hsp-4 takes place at A…

   Promoter

   Available since

   Sept. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGLOW31

   Plasmid

   #172698

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-3::mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Worm

   Mutation

   Promoter

   myo-3

   Available since

   Aug. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. Punc-4c.TIR1

   Plasmid

   #194053

   Purpose

   TIR1 expression vector under the unc-4c promoter expressed in the DA motor neurons in C. elegans

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   TIR1 (TIR1 Mustard Weed)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   codon-optimized for expression in C. elegans; Two…

   Promoter

   unc-4c

   Available since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJW6.01

   Plasmid

   #62882

   Purpose

   Transgene plasmid for expressing GFP::MEG-3 under meg-3 promoter and 3'UTR

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   GFP::MEG-3
   

   Use

   Tags

   GFP

   Expression

   Worm

   Mutation

   Promoter

   meg-3

   Available since

   March 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCFJ1320

   Plasmid

   #84829

   Purpose

   Minimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 introns

   Depositor

   Andrew Fire

   Article

   Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

   Insert

   C. elegans codon optimized GFP
   

   Use

   Tags

   Expression

   Bacterial and Worm

   Mutation

   Promoter

   Peft-3

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCZGY405

    Plasmid

    #172694

    Purpose

    Punc-25-mcherry::rab-3 Expresses Red-tagged synapses specifically in C. elegans GABAergic motor neurons

    Depositor

    Yishi Jin

    Article

    Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

    Insert

    rab-3 (rab-3 Nematode)
    

    Use

    Tags

    Punc-25 and mcherry

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNM3755

    Plasmid

    #154116

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 & an act-4 3' UTR in a RMCE vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::act-4 3'
    

    Use

    Rmce

    Tags

    Expression

    Worm

    Mutation

    Promoter

    not applicable

    Available since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEG345

    Plasmid

    #40077

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (mex-5 Nematode)
    

    Use

    Tags

    Dendra2 PFAF, S-peptide, and TEV protease cleavag…

    Expression

    Worm

    Mutation

    Promoter

    pie-1

    Available since

    Sept. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

13. pDACR3882 [rab-3p::HYlight::let-858utr]

    Plasmid

    #207666

    Purpose

    Expresses worm-optimized HYlight in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight Biosensor
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDACR3883 [rab-3p::HYlight-RA::let-858utr]

    Plasmid

    #207667

    Purpose

    Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

    Depositor

    Daniel Colón-Ramos

    Article

    Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

    Insert

    HYlight-RA [T152E]
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    rab-3

    Available since

    Feb. 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMS178

    Plasmid

    #215678

    Purpose

    Empty repair plasmid for ChrII split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxP + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMS114

    Plasmid

    #215677

    Purpose

    Empty repair plasmid for ChrI split hygromycinR landing pad

    Depositor

    Patrick Phillips

    Article

    Moerdyk-Schauwecker et al MicroPubl Biol. 2024 Jan 29;2024:10.1791

    Insert

    5'HA + MCS + loxN + rps-0p::5'ΔHygR
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    5' ∆HYGR encodes aa1-226

    Promoter

    Available since

    March 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPD_Pinx-1::ChR2(H134R)::mCherry

    Plasmid

    #117420

    Purpose

    For selectively expressing ChR2(H134R) in AiB interneurons under the expression of a inx-1 promoter

    Depositor

    Shohei Mitani

    Article

    Hori et al PLoS Genet. 2018 Jun 25;14(6):e1007477.

    Insert

    channel rhodopsin-2 with H134R mutation
    

    Use

    Tags

    Expression

    Worm

    Mutation

    H134R

    Promoter

    inx-1

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEY41

    Plasmid

    #191045

    Purpose

    dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    dat-1 (dat-1 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEY43

    Plasmid

    #191047

    Purpose

    eat-4(prom6-1) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

    Depositor

    Eviatar Yemini, Oliver Hobert

    Article

    Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

    Insert

    eat-4(prom6-1) (eat-4 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    None

    Promoter

    Available since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCZGY2727

    Plasmid

    #193330

    Purpose

    Site specific insertion using CRISPR/Cas9 editing of C. elegans ChrI

    Depositor

    Yishi Jin

    Article

    Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

    Insert

    Hygromycin resistance
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Prps-0

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge