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pDACR3883 [rab-3p::HYlight-RA::let-858utr]

Plasmid

#207667

Purpose

Expresses HYlight-RA, the negative control HYlight variant with reduced binding affinity, in C. elegans neurons

Depositor

Daniel Colón-Ramos

Article

Wolfe et al Proc Natl Acad Sci U S A. 2024 Jan 16;12

Insert

HYlight-RA [T152E]

Expression

Worm

Promoter

rab-3

Available Since

Feb. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pPD95_75

Plasmid

#1494

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, gfpN, IVSR, gfpS65C, IVSS, IVST, gfpC, IVSL…

Expression

Worm

Available Since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pDpy-7::oxCerulean-oxVenus::Lgg-1

Plasmid

#68042

Purpose

Lgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagy

Depositor

Dana Miller

Article

Chapin et al Aging (Albany NY). 2015 Jun 30.

Insert

pDpy-7::oxCerulean-oxVenus::Lgg-1

Expression

Worm

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pCFJ1955

Plasmid

#84830

Purpose

Minimos transposon with Peft-3:tdTomato:tbb-2 3'UTR and cbr-unc-119 selection. tdTomato was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic introns

Depositor

Andrew Fire

Article

Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

Insert

C. elegans codon optimized tdTomato

Expression

Bacterial and Worm

Promoter

Peft-3

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCZGY546

Plasmid

#90466

Purpose

Pmec-4::mcherry, Expresses mcherry red fluorescence in C. elegans touch neurons

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

Pmec-4-mcherry

Tags

mcherry

Expression

Worm

Promoter

Pmec-4

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDD363

Plasmid

#91829

Purpose

LoxP-flanked SEC donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

LoxP-SEC-LoxP

Use

CRISPR and Cre/Lox

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEY88

Plasmid

#191081

Purpose

ggr-1 fluorescent neural reporter driving nuclear CYOFP expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

ggr-1

Expression

Worm

Mutation

None

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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TetR in pENTR1A (559-1)

Plasmid

#22265

Depositor

Eric Campeau

Insert

Tetracycline repressor (tetR )

Expression

Insect, Mammalian, Worm Exp…

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pJF5

Plasmid

#139031

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

gfp (no STOP codon, PATC introns)

Expression

Worm

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pGLOW39

Plasmid

#173068

Purpose

mScarlet^SEC^3xMyc vector with ccdB sites for cloning homology arms

Depositor

Ryan Doonan

Article

Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

Insert

mScarlet-I-C1^SEC^3xMyc

Use

CRISPR and Cre/Lox

Tags

3xMyc and C. elegans codon-optimized mScarlet

Expression

Worm

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLZ31 (pCFJ151_Peft-3_TIR1_linker_mRuby_unc-54 3'UTR)

Plasmid

#71720

Purpose

Expressing an mRuby-tagged TIR1 in the soma of C. elegans

Depositor

Abby Dernburg

Article

Zhang et al Development. 2015 Nov 9. pii: dev.129635

Insert

TRANSPORT INHIBITOR RESPONSE 1 (TIR1 Mustard Weed)

Tags

mRuby

Expression

Worm

Mutation

codon-optimized for expression in C. elegans; Two…

Promoter

eef-1A.1 (eft-3)

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pSF11-wElectra2

Plasmid

#184933

Purpose

Codon optimized blue fluorescent protein Electra2 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra2

Expression

Worm

Promoter

tag-168

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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Prig-3::CD4::spGFP1-10

Plasmid

#65829

Purpose

For membrane contact

Depositor

Cori Bargmann, Kang Shen

Article

Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

Insert

Homo sapiens CD4 molecule (CD4), transcript variant 2, mRNA (CD4 Human)

Tags

spGFP1-10

Expression

Worm

Promoter

Prig-3

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only

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pJH23

Plasmid

#159314

Purpose

Punc-25 GFP::SYD-2

Depositor

Mei Zhen

Article

Yeh et al J Neurosci. 2005 Apr 13;25(15):3833-41.

Insert

syd-2 (syd-2 Nematode)

Tags

GFP

Expression

Worm

Promoter

Punc-25

Available Since

Sept. 22, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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L4440gtwy

Plasmid

#11344

Depositor

Guy Caldwell

Insert

gateway cassette

Use

RNAi

Expression

Worm

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pDD372

Plasmid

#91824

Purpose

Codon-optimized GFP donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

GFP-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMLS134

Plasmid

#73714

Purpose

SapTrap destination vector for building sgRNA expression vectors.

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pSM10

Plasmid

#139034

Purpose

MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

Depositor

Erik Griffin

Article

Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

Insert

mScarlet (no STOP codon)

Expression

Worm

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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