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8,350 results

Showing: 6601 - 6650 of 8350 results

  1. CPH3569 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,K1487N,D1497K; msLink2)

    Plasmid
    #91820
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available Since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91820-plasmid-map-sequence-id-181105

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  16. Dcp2 ΔN

    Plasmid
    #163575
    Purpose
    express wild type Dcp2 ΔN(243-970) at endogenous level in S.cerevisiae
    Depositor
    Roy Parker
    Article
    Xing et al Elife. 2020 Jun 19;9. pii: 56525. doi: 1
    Insert
    Dcp2 (DCP2 Budding Yeast)
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Truncation of N-terminal domain (1-242) of Dcp2
    Promoter
    DCP2 promoter
    Available Since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    163575-plasmid-map-sequence-id-323989

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  23. pPEE42

    Plasmid
    #139421
    Purpose
    Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end.
    Depositor
    James Fraser
    Article
    Spencer et al Fungal Genet Biol. 2020 May;138:103365.
    Insert
    Citrine
    Expression
    Yeast
    Promoter
    TEF1
    Available Since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    139421-plasmid-map-sequence-id-324126

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  41. VanR - F165V

    Plasmid
    #158965
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Valine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165V
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158965-plasmid-map-sequence-id-319279

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  42. VanR - F165R

    Plasmid
    #158966
    Purpose
    Wild type VanR where the residue in position 165 was mutated from Phenylalanine to Arginine. This mutation abolished the response to vanillic acid. The gene is under the control of the TEF1 promoter.
    Depositor
    Michael Jensen
    Article
    et al Biotechnology Notes, Volume 1, 2020, Pag
    Insert
    VanR - F165R
    Expression
    Yeast
    Mutation
    Wild type VanR from Caulobacter crescentus with m…
    Promoter
    TEF1
    Available Since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    158966-plasmid-map-sequence-id-319292

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  50. pCL21-decoder

    Plasmid
    #159448
    Purpose
    Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pact1-lexA-ER-B112-phosphodegron-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    act1
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159448-plasmid-map-sequence-id-313196

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Showing: 6601 - 6650 of 8350 results