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Plasmid#32440DepositorInsertUBC4 (UBC4 Budding Yeast)
UseTagsExpressionYeastMutationPromoterUBC4Available sinceSept. 27, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046406
Plasmid#153880PurposeYeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
FRP1454
Plasmid#64510PurposeExpresses destabilized Citrine in yeast.DepositorInsertCitrine-ADH1-tail
UseTagsExpressionYeastMutationPromoterTDH3Available sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCfB6679
Plasmid#106158PurposeEasyCloneYALI system-based yeast integrative vector for markerfree integration via CRISPR/Cas9 to be used in combination with pCfB6638, integration into Yarrowia lipolytica chromosomal location IntE_4, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046463
Plasmid#145462PurposeYeast Expression plasmid for SARS-CoV-2 nsp10DepositorInsertSARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable thrombin;6xHISExpressionYeastMutationwtPromoterpGAL1Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
G30, Cdc20-mCherry-AID tagging
Plasmid#130270PurposeTagging the Saccharomyces cerevisiae Cdc20 gene with a mCherry and auxin-degron tag, via homologous recombinationDepositorInsertCdc20/mCherry-AID(71-114)-tCYC1-NatMX (CDC20 Budding Yeast, Synthetic)
UseTagsAID(71-114) and mCherryExpressionYeastMutationPromoterNative promoterAvailable sinceSept. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-NatMx
Plasmid#122903PurposeTRP1-NatMx ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS403-PGAL1-weakSC_GFP
Plasmid#22315DepositorInsertGFP-rad9
UseIntegrating plasmidTagsExpressionYeastMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUDP044
Plasmid#101168PurposepUDP004 expressing a polycistronic g-RNA array for Cas9 editing targeting the genes SeATF1 and SeATF2 and Spcas9D147Y P411T in S. pastorianus (HH-gRNASeATF1-HDV-linker-HH-gRNASeATF2-HDV)DepositorInsertpolycistronic HH-gRNA-HDV-HH-gRNA-HDV array targetting SeATF1 and 2 in S. pastorianus
UseCRISPRTagsExpressionYeastMutationPromoterScTDH3Available sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB777
Plasmid#60153PurposeRed click beetle luciferase (Promega) driven by LEU1 promoter.DepositorInsertCBR
UseTagsExpressionYeastMutationPromoterLEU1prAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJCHph
Plasmid#185863PurposeThe CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable markerDepositorInsertPTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV0773
Plasmid#133519PurposeExpression of sfGFP targeted to the endoplasmic reticulum with a signal sequence and a retention signal (AHDL)DepositorInsertpLys3(BstZ17I)-p(BiP)-SignalSequenceBiP-sfGFP-AHDL-bsdMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCfB2195
Plasmid#67548PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XI-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAV0662
Plasmid#133477PurposeExpression in fission yeast from promoter pact1, integrated at lys3 locusDepositorInsertpLys3(BstZ17I)-p(act1)-sfGFP-terminator(ScCYC1)
UseTagsExpressionYeastMutationPromoterAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
HEL binding scFv D44.1
Plasmid#111718PurposeYeast Surface Display of D44.1 as a reference starting point for designDepositorInsertD44.1
UseTagscmyc tagExpressionYeastMutationPromoterAvailable sinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAG425GPD-ECFP-ccdB
Plasmid#14298DepositorTypeEmpty backboneUseGateway destinationTagsECFPExpressionYeastMutationPromoterAvailable sinceJune 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4H
Plasmid#104503PurposeUsed to evaluate the expression output of Saccharomyces arboricola GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSaGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4J
Plasmid#104505PurposeUsed to evaluate the expression output of Saccharomyces mikatae GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSmGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4N
Plasmid#104509PurposeUsed to evaluate the expression output of Saccharomyces bayanus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSbGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4R
Plasmid#104513PurposeUsed to evaluate the expression output of Saccharomyces uvarum GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSuGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only