8,133 results

1. pRS406-PHO5-GFP-CERT PH domain

   Plasmid

   #58725

   Purpose

   Expresses GFP-Tagged CERT PH domain in yeast

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   collagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
   

   Tags

   GFP and Myc

   Expression

   Yeast

   Promoter

   PHO5

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCfB2802

   Plasmid

   #63655

   Purpose

   pCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.

   Depositor

   Irina Borodina, Jerome Maury

   Article

   Maury et al PLoS One. 2016 Mar 2;11(3):e0150394. doi

   Insert

   GFP
   

   Use

   Cre/Lox

   Expression

   Yeast

   Promoter

   TEF1 promoter

   Available Since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSec7-Halox2

   Plasmid

   #115425

   Purpose

   Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

   Depositor

   Benjamin Glick

   Article

   Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

   Insert

   Sec7-Halox2
   

   Tags

   HaloTag

   Expression

   Yeast

   Promoter

   Endogenous

   Available Since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHLK

   Plasmid

   #64182

   Purpose

   harbours marker genes HIS3, LEU2 and LYS2

   Depositor

   Markus Ralser

   Article

   Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

   Type

   Empty backbone

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKT-SCFP3A-HIS

   Plasmid

   #64686

   Purpose

   pKT vector encoding yeast-codon optimized and fast-folding cyan fluorescent protein SCFP3A.

   Depositor

   Erin O'Shea

   Article

   Hansen et al Mol Syst Biol. 2013 Nov 5;9:704. doi: 10

   Insert

   SCFP3A
   

   Expression

   Yeast

   Mutation

   Nucleotide sequence has been yeast-codon optimize…

   Available Since

   April 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. LHP424-YCplac33-STE2-WT

   Plasmid

   #32171

   Depositor

   Linda Hicke

   Insert

   STE2 (STE2 Budding Yeast)
   

   Expression

   Yeast

   Promoter

   STE2

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pAG425GAL-ccdB-DsRed

   Plasmid

   #14369

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   DsRed

   Expression

   Yeast

   Available Since

   June 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. LD231

   Plasmid

   #69193

   Purpose

   Integration of 2GFP tag at Atg31 C terminus, use auxotrophic marker URA3(Candida albicans)

   Depositor

   Zhiping Xie

   Article

   Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

   Insert

   Atg31-2EGFP
   

   Tags

   2xEGFP

   Expression

   Yeast

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBKWH-Lam

   Plasmid

   #133899

   Purpose

   Negative control. Expression of Gal4BD-Lam hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Insert

   LMNA (Lam) (LMNA Human)
   

   Expression

   Yeast

   Promoter

   T7

   Available Since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pIRTetR-GAL80

    Plasmid

    #185853

    Purpose

    Plugging the mechanism of tetracycline-mediated repression of GAL promoters in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    gal80Arm1-PAgTEF1-KanMX4-TAgTEF1-PHAC1>TetR>TUP1>PABF1- PTEF1+[TetO]>GAL80Arm2
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179077

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosol

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Neon(gfp)
    

    Expression

    Yeast

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPS2167

    Plasmid

    #1365

    Depositor

    Pamela Silver

    Article

    Stage-Zimmermann et al Mol Biol Cell 2000 Nov;11(11):3777-89.

    Insert

    RPL11B (RPL11B Budding Yeast)
    

    Tags

    GFP

    Expression

    Yeast

    Available Since

    Aug. 3, 2005

    Availability

    Academic Institutions and Nonprofits only

14. LD111

    Plasmid

    #69165

    Purpose

    Integration of GFP tag at Atg11 C terminus, use auxotrophic marker URA3(Candida albicans)

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    Atg11-EGFP
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAG416GPD-ccdB-TAP

    Plasmid

    #14268

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    TAP

    Expression

    Yeast

    Available Since

    June 18, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pXP412

    Plasmid

    #26840

    Depositor

    Nancy DaSilva, Suzanne Sandmeyer

    Article

    Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Dec. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDN-G1TCbt

    Plasmid

    #44515

    Depositor

    Gabor Balazsi

    Article

    Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

    Inserts

    PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
    tetR::ymcherry
    

    Use

    Synthetic Biology; Expression regulator/reporter

    Expression

    Yeast

    Mutation

    Two tandem tet operators (2XtetO2) downstream of …

    Promoter

    PGal1-D12 promoter

    Available Since

    April 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. 416Gal-FUS-R521H-YFP

    Plasmid

    #29624

    Depositor

    Aaron Gitler

    Article

    Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

    Insert

    FUS (FUS Human)
    

    Tags

    YFP

    Expression

    Yeast

    Mutation

    R521H

    Available Since

    April 27, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pJK369

    Plasmid

    #73594

    Purpose

    Encodes Crepis alpina delta-12 fatty acid acetylenase (vFAD2) with C-terminal FLAG epitope

    Depositor

    T. J. Kappock

    Insert

    delta-12 fatty acid acetylenase
    

    Tags

    FLAG tag

    Expression

    Yeast

    Promoter

    GAL10

    Available Since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. P413TEF-PYK1

    Plasmid

    #34605

    Depositor

    Markus Ralser

    Article

    Gruning et al Cell Metab. 2011 Sep 7;14(3):415-27.

    Insert

    PYK1
    

    Expression

    Yeast

    Promoter

    TEF

    Available Since

    Jan. 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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