7,943 results
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Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-S2197F
Plasmid#89967PurposeS2197F point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationS2197FPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-H2387P
Plasmid#89968PurposeH2387P point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationH2387PPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS313-prp8-R2388G
Plasmid#89969PurposeR2388G point mutantDepositorInsertPRP8 (PRP8 Budding Yeast)
UseTagsExpressionYeastMutationR2388GPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-1
Plasmid#89972Purposeprp16-1 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-1 allele (Y386D)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pRS314-prp16-101
Plasmid#89973Purposeprp16-101 allele in TRP plasmidDepositorInsertPRP16 (PRP16 Budding Yeast)
UseTagsExpressionYeastMutationprp16-101 allele (G373S)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL3 - Brr2 del113 N1104L
Plasmid#90253Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 113 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL7 - Brr2 del247 N1104L
Plasmid#90257Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 247 amino acids and N1104LPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL11 - Brr2 del422 N1104L
Plasmid#90261Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid N110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL12 - Brr2 del422 Q904E
Plasmid#90262Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid Q904EPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pSL13 - Brr2 del422 R1107L
Plasmid#90263Purposeprotein expressionDepositorInsertBRR2
UseTagsTAPExpressionYeastMutationdeleted first 422 amino acids and amino acid R110…PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pHES942
Plasmid#90408PurposeExpresses SIC1 udner control of synthetic promoter containing Zif268 binding sites.DepositorInsertpZ-SIC1
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-3 in pRS314
Plasmid#90454PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-3 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-4 in pRS314
Plasmid#90455PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-4 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
Sub2-6 in pRS314
Plasmid#90456PurposeyeastDepositorInsertSUB2
UseTagsExpressionYeastMutationsub2-6 mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
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brr2-Q904E pRS313
Plasmid#111413Purposebrr2-Q904E (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationQ904E aa mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107A pRS313
Plasmid#111415Purposebrr2-R1107A (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107A aa mutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
brr2-R1107M pRS313
Plasmid#111417Purposebrr2-R1107M (endogenous promoter)DepositorInsertBRR2
UseTagsExpressionYeastMutationR1107M aa mutation (note, not an RP allele)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pV1338
Plasmid#111443PurposeSolo vector pV1326 + sgScADE2DepositorInsertCaCas9/sgScADE2
UseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
yFusionRed
Plasmid#111916Purposeyeast optimized FusionRedDepositorInsertyFusionRed
UseTagsExpressionYeastMutationPromoterGPDAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1668
Plasmid#112042PurposeCEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URADepositorInsertFET3
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1669
Plasmid#112043PurposeCEN/ARS plasmid for GAL-inducible NSG2-V5-TEV-EGFP expression marked with URADepositorInsertNSG2
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1672
Plasmid#112046PurposeCEN/ARS plasmid for GAL-inducible PST1-V5-TEV-EGFP expression marked with URADepositorInsertPST1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pJW1674
Plasmid#112048PurposeCEN/ARS plasmid for GAL-inducible SWP1-V5-TEV-EGFP expression marked with URADepositorInsertSWP1
UseTagsV5-TEV-EGFPExpressionYeastMutationPromoterGAL1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4G
Plasmid#104502PurposeUsed to evaluate the expression output of Saccharomyces cerevisiae PGM2 promoter with yEGFP used as the reporter geneDepositorInsertScPGM2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4K
Plasmid#104506PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL1 promoter with yEGFP used as the reporter geneDepositorInsertSpGAL1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4O
Plasmid#104510PurposeUsed to evaluate the expression output of Saccharomyces paradoxus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSpdGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pILGFP4P
Plasmid#104511PurposeUsed to evaluate the expression output of Saccharomyces pastorianus GAL2 promoter with yEGFP used as the reporter geneDepositorInsertSptGAL2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pEMY01AB-PRO
Plasmid#127714PurposeIntegrative yeast promoter characterization plasmid. Clone in with BpiI. Has ChrXV homology upstream and an mVenus fragment downstream.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tadh1
Plasmid#127723PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and ADH1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Ppre3-ITE-Teno1
Plasmid#127724PurposeYeast pathway position 1. ITE transcription unit with the PRE3 promoter and ENO1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPpre3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Pact1-ITE-Tdpp1
Plasmid#127725PurposeYeast pathway position 1. ITE transcription unit with the ACT1 promoter and DPP1 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPact1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-5a-Phta1-ITE-Tvma2
Plasmid#127726PurposeYeast pathway position 1. ITE transcription unit with the HTA1 promoter and VMA2 terminator.DepositorInsertitaconate transporter from A. terreus
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPhta1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE ADE2-MET17
Plasmid#122885PurposeADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE LYS2-LEU2
Plasmid#122888PurposeLYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-HIS3
Plasmid#122891PurposeTRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pICE TRP1-MET17
Plasmid#122893PurposeTRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-MET17
Plasmid#122910PurposeLYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-URA3
Plasmid#122911PurposeLYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-LEU2
Plasmid#122913PurposeTRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
YIp TRP1-MET17
Plasmid#122914PurposeTRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
YIp LYS2-KanMx
Plasmid#122920PurposeLYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.DepositorTypeEmpty backboneUseYeast recombination cloningTagsExpressionYeastMutationPromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Prps3-CAD-Tcyc1
Plasmid#127728PurposeYeast pathway position 3. CAD transcription unit with the RPS3 promoter and CYC1 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPrps3Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Peft2-CAD-Tklura3
Plasmid#127729PurposeYeast pathway position 3. CAD transcription unit with the EFT2 promoter and K. lactis URA3 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPeft2Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-bc-Psmtef1-CAD-Tyhi9
Plasmid#127730PurposeYeast pathway position 3. CAD transcription unit with the S. mikatae TEF1 promoter and YHI9 terminator.DepositorInsertcis-aconitate decarboxylase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPsmtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pfba1p-ACO-Tgpm1
Plasmid#127731PurposeYeast pathway position 4. ACO transcription unit with the FBA1 promoter and GPM1 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPfba1Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Pykt6-ACO-Tprm9
Plasmid#127732PurposeYeast pathway position 4. ACO transcription unit with the YKT6 promoter and PRM9 terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPykt6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pDSM-cd-Ptef1-ACO-Tyol036w
Plasmid#127733PurposeYeast pathway position 4. ACO transcription unit with the TEF1 promoter and YOL036w terminator.DepositorInsertaconitase
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPtef1Available sinceAvailabilityAcademic Institutions and Nonprofits only