7,943 results

1. pDSM-f3-Prpl28-ACDH-Tecm10

   Plasmid

   #127745

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the RPL28 promoter and ECM10 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Prpl28

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDSM-f3-Ptdh3-ACDH-Trpl3

   Plasmid

   #127746

   Purpose

   Yeast pathway position 7. ACDH transcription unit with the TDH3 promoter and RPL3 terminator.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Insert

   acetylating acetaldehyde dehydrogenase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Ptdh3

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEMY01CD-TER

   Plasmid

   #127751

   Purpose

   Integrative yeast terminator characterization plasmid. Clone in with BpiI. Has mVenus fragment upstream and a KanMX fragment downstream.

   Depositor

   Christopher Voigt

   Article

   Young et al Metab Eng. 2018 Jul;48:33-43. doi: 10.10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pICE ADE2-MET17

   Plasmid

   #122885

   Purpose

   ADE2-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pICE LYS2-LEU2

   Plasmid

   #122888

   Purpose

   LYS2-LEU2 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pICE TRP1-HIS3

   Plasmid

   #122891

   Purpose

   TRP1-HIS3 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pICE TRP1-MET17

   Plasmid

   #122893

   Purpose

   TRP1-MET17 ICE plasmid used to facilitate yeast recombination cloning and stable genomic Integration after CEN Excision.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. YIp LYS2-MET17

   Plasmid

   #122910

   Purpose

   LYS2-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YIp LYS2-URA3

   Plasmid

   #122911

   Purpose

   LYS2-URA3 integrating (YIp) vector for stable genomic integration in the BY4741 background.

   Depositor

   Randy Hampton

   Article

   Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

   Type

   Empty backbone

   Use

   Yeast recombination cloning

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. YIp TRP1-LEU2

    Plasmid

    #122913

    Purpose

    TRP1-LEU2 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YIp TRP1-MET17

    Plasmid

    #122914

    Purpose

    TRP1-MET17 integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. YIp LYS2-KanMx

    Plasmid

    #122920

    Purpose

    LYS2-KanMx integrating (YIp) vector for stable genomic integration in the BY4741 background.

    Depositor

    Randy Hampton

    Article

    Flagg et al Yeast. 2019 May 10. doi: 10.1002/yea.340

    Type

    Empty backbone

    Use

    Yeast recombination cloning

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. p414GPD-3xFLAG-Nop4 L306P

    Plasmid

    #169262

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 L306P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 L306P (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu306Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p414GPD-3xFLAG-Nop4 dE5

    Plasmid

    #169263

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E5 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. p414GPD-3xFLAG-Nop4 dE8

    Plasmid

    #169264

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-Nop4 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    Nop4 delta E8 (NOP4 Budding Yeast)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted region corresponding to human Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p414GPD-3xFLAG-RBM28 WT

    Plasmid

    #169265

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 WT

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 WT (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. p414GPD-3xFLAG-RBM28 L351P

    Plasmid

    #169266

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 L351P

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 L351P (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    p.(Leu351Pro)

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p414GPD-3xFLAG-RBM28 dE5

    Plasmid

    #169267

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 5

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E5 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 5

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p414GPD-3xFLAG-RBM28 dE8

    Plasmid

    #169268

    Purpose

    S. cerevisiae (yeast) vector for constitutive expression of 3xFLAG-RBM28 delta Exon 8

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 delta E8 (RBM28 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Yeast

    Mutation

    deleted Exon 8

    Promoter

    GPD

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHCMM1

    Plasmid

    #167452

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the TEF1 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PTEF1_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TEF1

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHCMM2

    Plasmid

    #167453

    Purpose

    Expresses C.elegans PUP-2 linked to RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (+PAB)] from the Sec63 promoter

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_scPAB14RRMS_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pHCMM3

    Plasmid

    #167454

    Purpose

    A control chimera; expresses C. elegans PUP-2 from the Sec63 promoter without the RNA-recognition motifs (RRMs) of yeast poly(A)-binding protein [PUP alone (-PAB)]

    Depositor

    Marvin Wickens, Hugo Cesar Medina-Munoz

    Article

    Medina-Munoz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

    Insert

    PSEC63_yeGFP_cePUP-2_URA3_TADH1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PSEC63

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. WT Ecm2

    Plasmid

    #169922

    Purpose

    Ecm2 +/-300bp of up- and downstream seq. cloned into the NotI and SalI sites of pRS416

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. Ecm2 1-143

    Plasmid

    #169923

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 143 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-143
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Ecm2 1-265

    Plasmid

    #169925

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 265 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-265
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Ecm2 1-325

    Plasmid

    #169926

    Purpose

    WT Ecm2 mutated to introduce stop codon after AA 325 of Ecm2

    Depositor

    Aaron Hoskins

    Article

    van der Feltz et al RNA. 2021 Feb 5. pii: rna.077727.120. do

    Insert

    Ecm2 1-325
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRS313-prp8-S2197F

    Plasmid

    #89967

    Purpose

    S2197F point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    S2197F

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pRS313-prp8-H2387P

    Plasmid

    #89968

    Purpose

    H2387P point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H2387P

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRS313-prp8-R2388G

    Plasmid

    #89969

    Purpose

    R2388G point mutant

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    R2388G

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRS314-prp16-1

    Plasmid

    #89972

    Purpose

    prp16-1 allele in TRP plasmid

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP16 (PRP16 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    prp16-1 allele (Y386D)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pRS314-prp16-101

    Plasmid

    #89973

    Purpose

    prp16-101 allele in TRP plasmid

    Depositor

    Christine Guthrie

    Article

    Mayerle et al RNA. 2016 May;22(5):793-809. doi: 10.126

    Insert

    PRP16 (PRP16 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    prp16-101 allele (G373S)

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pRS313 - prp8 V1860N

    Plasmid

    #90966

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V1860N

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pRS313 - prp8 V1862D

    Plasmid

    #90967

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V1862D

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pRS313 - prp8 T1865K

    Plasmid

    #90968

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T1865K

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pRS313 - prp8 A1871E

    Plasmid

    #90969

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    A1871E

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pRS313 - prp8 T1872E

    Plasmid

    #90970

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T1872E

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRS313 - prp8 T1861P

    Plasmid

    #90971

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T1861P

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRS313 - prp8 V1862Y

    Plasmid

    #90972

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V1862Y

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pRS313 - prp8 H1863E

    Plasmid

    #90973

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    H1863E

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pRS313 - prp8 K1864E

    Plasmid

    #90974

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    K1864E

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pRS313 - prp8 N1869D

    Plasmid

    #90975

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    N1869D

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pRS313 - prp8 V1870N

    Plasmid

    #90976

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    V1870N

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pRS313 - prp8 I1875T

    Plasmid

    #90977

    Purpose

    yeast vector

    Depositor

    Christine Guthrie

    Article

    Mayerle et al Proc Natl Acad Sci U S A. 2017 Apr 17. p

    Insert

    PRP8 (PRP8 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    I1875T

    Promoter

    Available since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge