8,047 results

1. pJT171_GalL_A3AΔ178-BE3

   Plasmid

   #145121

   Purpose

   Expressing base editor A3AΔ178-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3AΔ178-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(1-178AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJT170_GalL_A3AΔ182-BE3

   Plasmid

   #145120

   Purpose

   Expressing base editor A3AΔ182-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3AΔ182-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(1-182AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJT169_GalL_A3AΔ186-BE3

   Plasmid

   #145119

   Purpose

   Expressing base editor A3AΔ186-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3AΔ186-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   A3A(1-186AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJT166_GalL_A3A-BE3

   Plasmid

   #145116

   Purpose

   Expressing base editor A3A-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   A3A-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJT139_GalL_nCDA1-VRERBE3

   Plasmid

   #145102

   Purpose

   Expressing base editor nCDA1-VRERBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   nCDA1-VRERBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A/D1135V/G1218R/R1335E/T1337R)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJT122_GalL_cCDA1-VQRBE3

   Plasmid

   #145087

   Purpose

   Expressing base editor cCDA1-VQRBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-VQRBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, D1135V, R1335Q, T1337R)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJT113_GalL_cCDA1-xBE3

   Plasmid

   #145079

   Purpose

   Expressing base editor cCDA1-xBE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

   Insert

   cCDA1-xBE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJT92_GalL_nCDA1Δ188-BE3

   Plasmid

   #145052

   Purpose

   Expresses nCDA1Δ188-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ188-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-188AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pJT90_GalL_nCDA1Δ190-BE3

   Plasmid

   #145050

   Purpose

   Expresses nCDA1Δ190-BE3 in yeast cells

   Depositor

   Ralph Bock

   Article

   Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

   Insert

   nCDA1Δ190-BE3
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   pmCDA1(1-190AA); spCas9(D10A)

   Promoter

   GalL

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJT85_GalL_nCDA1Δ193-BE3

    Plasmid

    #145048

    Purpose

    Expresses nCDA1Δ193-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ193-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-193AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pJT84_GalL_nCDA1Δ194-BE3

    Plasmid

    #145047

    Purpose

    Expresses nCDA1Δ194-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ194-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-194AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pJT78_GalL_nCDA1Δ195-BE3

    Plasmid

    #145044

    Purpose

    Expresses nCDA1Δ195-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

    Insert

    nCDA1Δ195-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    pmCDA1(1-195AA); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4046638

    Plasmid

    #153941

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4046439

    Plasmid

    #153938

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp4

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp4 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046301

    Plasmid

    #153925

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046274

    Plasmid

    #153927

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046639

    Plasmid

    #153928

    Purpose

    Yeast expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046537

    Plasmid

    #153914

    Purpose

    Yeast expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046343

    Plasmid

    #153915

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046617

    Plasmid

    #153917

    Purpose

    Yeast expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pGBW-m4046622

    Plasmid

    #153904

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

22. pGBW-m4046641

    Plasmid

    #153905

    Purpose

    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

23. pGBW-m4046334

    Plasmid

    #153901

    Purpose

    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pGBW-m4046590

    Plasmid

    #153902

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp7

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

25. pGBW-m4046172

    Plasmid

    #153903

    Purpose

    Yeast expression plasmid for SARS-CoV-2 nsp2

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 nsp2 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

26. pGBW-m4046178

    Plasmid

    #153891

    Purpose

    Yeast expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pGBW-m4046698

    Plasmid

    #153892

    Purpose

    Yeast expression plasmid for SARS-CoV-2 ORF3a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

28. pGBW-m4046618

    Plasmid

    #153893

    Purpose

    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pGBW-m4046226

    Plasmid

    #153889

    Purpose

    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pGBW-m4046350

    Plasmid

    #153890

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

31. pGBW-m4046705

    Plasmid

    #153881

    Purpose

    Yeast expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pGBW-m4046289

    Plasmid

    #153877

    Purpose

    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pGBW-m4046293

    Plasmid

    #153879

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pGBW-m4046365

    Plasmid

    #153867

    Purpose

    Yeast expression plasmid for SARS-CoV-2 ORF7a protein

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 ORF7a protein (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pGBW-m4046680

    Plasmid

    #153870

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

36. pGBW-m4046501

    Plasmid

    #153855

    Purpose

    Yeast expression plasmid for SARS-CoV-2 S (surface glycoprotein)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pGBW-m4046162

    Plasmid

    #153856

    Purpose

    Yeast expression plasmid for SARS-CoV-2 3'-to-5' exonuclease

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 3'-to-5' exonuclease (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;StrepII

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

38. pGBW-m4046670

    Plasmid

    #153858

    Purpose

    Yeast expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pGAL1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

39. pGBW-m4046279

    Plasmid

    #153854

    Purpose

    Yeast expression plasmid for SARS-CoV-2 2'-O-ribose methyltransferase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 2'-O-ribose methyltransferase (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;MBP

    Expression

    Yeast

    Mutation

    wt

    Promoter

    pTEF1

    Available since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pSP569

    Plasmid

    #139499

    Purpose

    Repair DNA plasmid to insert PP7 stem loops in the endogenous STL1 locus after double strand break in S. cerevisiae

    Depositor

    Serge Pelet

    Article

    Wosika et al Nat Commun. 2020 Jun 23;11(1):3171. doi:

    Insert

    pSTL1 24xPP7sl STL1 100 - 600
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    WT

    Promoter

    pSTL1

    Available since

    June 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge