8,057 results

1. pSNR52-sgTEF1

   Plasmid

   #46922

   Purpose

   Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TEF1 promoter

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgTEF1 promoter (TEF1 Budding Yeast)
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   SNR52

   Available since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGADT7-αC

   Plasmid

   #197650

   Purpose

   Expression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)

   Depositor

   Juan Bonifacino

   Article

   Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.

   Insert

   AP-2 αC
   

   Use

   Tags

   GAL4 transcriptional activation domain (AD) fragm…

   Expression

   Yeast

   Mutation

   Ser677T substitution

   Promoter

   ADH1

   Available since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYES2-HTH

   Plasmid

   #86470

   Purpose

   Yeast expression vector containing HIS10-TEV-HA (HTH) epitope tag downstream of the GAL1 promoter

   Depositor

   Akash Gunjan

   Article

   Singh et al Nat Cell Biol. 2009 Aug;11(8):925-33. do

   Type

   Empty backbone

   Use

   Tags

   HIS10-TEV-HA

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pRR-ERalpha-5Z

   Plasmid

   #23061

   Depositor

   Charles Miller

   Article

   Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

   Insert

   ERalpha (ESR1 Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 31, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pCS4333 yeTadA1.0-T7 RNAP

   Plasmid

   #137735

   Purpose

   pCS4333 CEN/ARS vector, PGAL1-yeTadA1.0-100AA linker-T7 RNAP-TCYC1, TRP1 selectable marker

   Depositor

   Christina Smolke

   Article

   Cravens et al Nat Commun. 2021 Mar 11;12(1):1579. doi:

   Insert

   yeTadA1.0-T7 RNAP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   E366G (See depositor comment below)

   Promoter

   Available since

   Jan. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDZ513 (pHIS MET25pro PP7-PS-yeGFP)

   Plasmid

   #45931

   Depositor

   Daniel Zenklusen

   Article

   Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

   Insert

   PCP-PS
   

   Use

   Tags

   2x-yeGFP

   Expression

   Yeast

   Mutation

   Promoter

   Met25

   Available since

   Oct. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFA6a- mEGFP-kanMX6

   Plasmid

   #105146

   Purpose

   Yeast gene targeting

   Depositor

   Thomas Pollard, Jian-Qiu Wu

   Article

   Wu et al Dev Cell. 2003 Nov;5(5):723-34.

   Insert

   mEGFP-kanMX6
   

   Use

   Tags

   mEGFP

   Expression

   Bacterial and Yeast

   Mutation

   A206K

   Promoter

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pETCON-HtsptLB12v.3

   Plasmid

   #45121

   Depositor

   David Baker

   Article

   Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

   Insert

   HtsptLB12v.3
   

   Use

   Tags

   Aga2p for yeast surface display and c-myc epitope…

   Expression

   Yeast

   Mutation

   Promoter

   GAL

   Available since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG425GAL-ccdB-EGFP

   Plasmid

   #14201

   Depositor

   Susan Lindquist

   Article

   Alberti et al Yeast. 2007 Oct . 24(10):913-9.

   Type

   Empty backbone

   Use

   Gateway destination

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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10. ZJOM149

    Plasmid

    #133646

    Purpose

    Integration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-ELO3-mCherry (ELO3 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pE2n

    Plasmid

    #17456

    Purpose

    modified pENTR vector for N-terminal 3x HA tag fusion with your gene of interest

    Depositor

    Giovanna Benvenuto

    Article

    Dubin et al Plant Methods. 2008 Jan 22. 4(1):3.

    Type

    Empty backbone

    Use

    Modified gateway entry vector

    Tags

    3xHA

    Expression

    Bacterial, Insect, Mammalia…

    Mutation

    Promoter

    Available since

    March 21, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZS165 CRISPEY HH-HDV NotI

    Plasmid

    #114451

    Purpose

    Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HH-HDV ribozyme

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Type

    Empty backbone

    Use

    CRISPR; Cen/ars

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScGAL7p

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBEVY-L

    Plasmid

    #51226

    Purpose

    bi-directional expression vector for yeast, constitutively active, LEU2 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD/ADH1

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUDP004

    Plasmid

    #101165

    Purpose

    E. coli/S. cerevisiae shuttle vector carrying amd S marker and Spcas9D147Y P411T allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

    Depositor

    Jean-Marc Daran

    Article

    de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScTDH3

    Available since

    Dec. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZJOM65

    Plasmid

    #133654

    Purpose

    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    

    Use

    Tags

    mCherry

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. LT1_34_sfpHluorin

    Plasmid

    #177707

    Purpose

    Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    pTEF1-sfpHluorin
    

    Use

    Used as donor in genome integration after lineari…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTO136_pADH1-tRNA-Ala-gRNA-tracrRNA-HDV-tAgTEF2

    Plasmid

    #171104

    Purpose

    Expresses sgRNA (ENO1 gRNA) in Candida auris.

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    sgRNA
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pIW601-KmCRISPR

    Plasmid

    #98907

    Purpose

    K. marxianus CRISPR Plasmid for sgRNA cloning

    Depositor

    Ian Wheeldon

    Article

    Lobs et al Biotechnol Biofuels. 2017 Jun 24;10:164.

    Inserts

    Codon optimized Cas9
    sgRNA expression cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    SV40

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTO135_pENO1-CaCas9-tCyc1

    Plasmid

    #171103

    Purpose

    Expresses Cas9 in Candida auris

    Depositor

    Teresa O'Meara

    Article

    Santana et al Nat Commun. 2021 Dec 10;12(1):7197. doi:

    Insert

    CaCas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ENO1

    Available since

    July 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYAL_HLA-A2_MART1

    Plasmid

    #171177

    Purpose

    Displays MART-1 peptide in HLA-A*02 as a single-chain trimer

    Depositor

    Michael Birnbaum

    Article

    Huisman et al Methods Mol Biol. 2022;2491:263-291. doi

    Insert

    MART1_HLA-A2*01 single-chain trimer fusion
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    GAL1

    Available since

    July 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge