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pML107-PMR1

Plasmid

#226264

Purpose

Plasmid expressing Cas9 and gRNA ACATGACCGTATCTAAACTT which targets the PMR1 gene

Depositor

Jolanda Van Leeuwen

Article

Paltenghi et al G3 (Bethesda). 2025 Mar 3:jkaf047. doi:

Insert

PMR1 gRNA (PMR1 Budding Yeast)

Use

CRISPR

Expression

Yeast

Available Since

Oct. 28, 2024

Availability

Academic Institutions and Nonprofits only

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UG75-DGK1-3HA

Plasmid

#184759

Purpose

DGK1 overexpression. Promotes the formation of phosphatidate from diacylglycerol. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

DGK1 (DGK1 Budding Yeast)

Tags

3HA

Expression

Yeast

Promoter

DGK1

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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UG76-pLYS2-GFP-PKCbeta

Plasmid

#184762

Purpose

Marker for DAG. Yeast expression of human PKC beta under LSY2 promoter. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

pLYS2-GFP-PKCbeta (PRKCB Human, Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Linked Free NES (M)

Plasmid

#182436

Purpose

Yeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al bioRxiv 2022.11.24.517869

Insert

FPPS(M)-NES

Use

Synthetic Biology; Metabolic engineering

Expression

Yeast

Promoter

GAL10

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Article

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJW1667

Plasmid

#112041

Purpose

CEN/ARS plasmid for GAL-inducible ERG5-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

ERG5

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pRS1244

Plasmid

#247644

Purpose

Inducible CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1074

Plasmid

#247642

Purpose

Constitutive CRISPRi-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pRS1242

Plasmid

#247643

Purpose

Inducible CRISPRa-HyperdCas12a for Candida albicans

Depositor

Rebecca Shapiro

Article

Gervais et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Type

Empty backbone

Use

CRISPR

Expression

Yeast

Available Since

Jan. 5, 2026

Availability

Academic Institutions and Nonprofits only

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pJRoC30_Th3

Plasmid

#188064

Purpose

PROSS-designed high-redox potential laccase from Trametes hirsuta

Depositor

Sarel Fleishman

Article

Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

Insert

PROSS-designed high-redox potential laccase from Trametes hirsuta

Tags

S. cerevisiae evolved α-factor prepro-leader sign…

Expression

Yeast

Mutation

20 mutations, described in publication

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pJW1670

Plasmid

#112044

Purpose

CEN/ARS plasmid for GAL-inducible OLE1-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

OLE1

Tags

V5-TEV-EGFP

Expression

Yeast

Mutation

P319T

Promoter

GAL1

Available Since

May 28, 2022

Availability

Academic Institutions and Nonprofits only

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pJW1668

Plasmid

#112042

Purpose

CEN/ARS plasmid for GAL-inducible FET3-V5-TEV-EGFP expression marked with URA

Depositor

Jonathan Weissman

Article

Costa et al Science. 2018 Jan 18. pii: science.aar36

Insert

FET3

Tags

V5-TEV-EGFP

Expression

Yeast

Promoter

GAL1

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pV1210

Plasmid

#242891

Purpose

Modified plasmid from pV1200 to perform ENO1-SI CRISPR in Candidozyma auris

Depositor

Patrick Van Dijck

Article

Sofras et al bioRxiv [Preprint]. 2025 Jan 11:2025.01.

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Feb. 19, 2026

Availability

Academic Institutions and Nonprofits only

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pFA6a-mIAA7-3xFLAG-HIS3MX6

Plasmid

#247614

Purpose

Yeast gene tagging with mIAA7

Depositor

Sebastian Schuck

Article

Hubbe et al Mol Biol Cell. 2026 Feb 1;37(2):mr2. doi

Insert

mIAA7

Tags

3xFLAG

Expression

Yeast

Available Since

Dec. 9, 2025

Availability

Academic Institutions and Nonprofits only

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pACT-HAB1_star

Plasmid

#241277

Purpose

Orthogonal PYR1/HAB1 sensor module

Depositor

Sean Cutler

Article

Park et al Nat Chem Biol. 2024 Jan;20(1):103-110. d

Insert

HAB1_star (HAB1 Mustard Weed)

Expression

Yeast

Mutation

NA

Available Since

Aug. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-Kan

Plasmid

#44903

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCHA-VRC01-scFv

Plasmid

#44874

Depositor

Dane Wittrup

Article

Mata-Fink et al J Mol Biol. 2013 Jan 23;425(2):444-56. d

Insert

VRC01 scFv

Tags

HA and Myc

Expression

Yeast

Promoter

GAL1

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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p403-BrdU-Inc

Plasmid

#71789

Purpose

BrdU-incorporating plasmid with selectable marker HIS3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pSFS2A-CaKan

Plasmid

#189565

Purpose

Codon optomized Kan resistance gene in the pSFS2A flipper backbone for genetic manipulation of Candida albicans using G418 selection

Depositor

Richard Bennett

Article

Park et al mSphere. 2022 Aug 15:e0034722. doi: 10.1

Insert

CaKan

Expression

Yeast

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pESC-leu2d(empty)

Plasmid

#20120

Depositor

Jay Keasling

Article

Ro et al Nature. 2006 Apr 13. 440(7086):940-3.

Type

Empty backbone

Use

Synthetic Biology

Expression

Yeast

Available Since

Feb. 12, 2009

Availability

Academic Institutions and Nonprofits only

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