8,085 results

1. pABY-T2

   Plasmid

   #201765

   Purpose

   For C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeast

   Depositor

   Saravanan Palani

   Article

   Akhuli et al mSphere. 2022 Oct 26;7(5):e0033322. doi:

   Insert

   ALFA tag
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRG203MX

   Plasmid

   #64521

   Purpose

   Integrative E. coli/S. cerevisiae shuttle vector

   Depositor

   Joerg Stelling

   Article

   Gnugge et al Yeast. 2015 Dec 9. doi: 10.1002/yea.3144

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Aug. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDR-GW FLIPglu-600μΔ13V

   Plasmid

   #28001

   Depositor

   Wolf Frommer

   Article

   Bermejo et al Biochem J. 2010 Dec 1. 432(2):399-406.

   Insert

   FLIPglu-600μΔ13V
   

   Use

   Tags

   eCFP and eYFP

   Expression

   Yeast

   Mutation

   F16A in mglB

   Promoter

   Available Since

   March 31, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pBF3060

   Plasmid

   #26853

   Purpose

   Expression of yeast codon-optimized CreA recombinase from the GAL1 promoter

   Depositor

   Nancy DaSilva, Suzanne Sandmeyer

   Article

   Fang et al Yeast. 2011 Feb;28(2):123-36. doi: 10.10

   Insert

   CreA recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   GAL1

   Available Since

   Dec. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYES1L-URA

   Plasmid

   #84301

   Purpose

   E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

   Depositor

   George Church

   Article

   Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available Since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBIS-GALkFLP(Trp1)

   Plasmid

   #49459

   Purpose

   overespression of Flp step-arrest mutant in yeast, TRP1 selection

   Depositor

   Marc Gartenberg

   Article

   Tsalik et al Yeast. 1998 Jun 30;14(9):847-52.

   Insert

   Flp(H305L) (flp Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   H305L, ‘step-arrest’ mutant,

   Promoter

   GAL10

   Available Since

   Nov. 22, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SIVt

   Plasmid

   #81092

   Purpose

   Single Integration Vector TRP - Cloning vector for single integration into the TRP1 locus of S.cerevisiae containing two MCS.

   Depositor

   Serge Pelet

   Article

   Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Nov. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Use

   Tags

   ymNeonGreen

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS42H_PTEF1_dCas9_TCYC1

   Plasmid

   #177155

   Purpose

   Expresses dCas9 in yeast cells

   Depositor

   Gautam Dantas

   Article

   Kwak et al ACS Synth Biol. 2022 Jan 21;11(1):508-51

   Insert

   Nuclease-null Cas9
   

   Use

   Tags

   SV40 NLS

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 promoter

   Available Since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. SIVh

    Plasmid

    #81091

    Purpose

    Single Integration Vector HIS - Cloning vector for single integration into the HIS3 locus of S.cerevisiae containing two MCS.

    Depositor

    Serge Pelet

    Article

    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

    Type

    Empty backbone

    Use

    Cre/Lox

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BS-Met15

    Plasmid

    #69198

    Purpose

    complementation of yeast auxotrophic marker MET15

    Depositor

    Zhiping Xie

    Article

    Li et al Autophagy. 2015;11(6):954-60. doi: 10.10

    Insert

    MET15
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pWL078 MCP-2xGFP

    Plasmid

    #197047

    Purpose

    pCYC-NLS-MCP-2xGFP on pRS305

    Depositor

    Robert Singer

    Article

    Li et al Nat Methods. 2022 Dec;19(12):1558-1562.

    Insert

    MCP-2xGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1

    Available Since

    March 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAG303-Gal-GA50

    Plasmid

    #84907

    Purpose

    induce expression of dipeptide repeat GA in yeast

    Depositor

    Aaron Gitler

    Article

    Jovicic et al Nat Neurosci. 2015 Sep;18(9):1226-9. doi

    Insert

    GA50 (C9orf72 Synthetic)
    

    Use

    Tags

    FLAG and Myc

    Expression

    Yeast

    Mutation

    Promoter

    GAL1

    Available Since

    Dec. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAHYB-GFP

    Plasmid

    #85074

    Purpose

    Expression of GFP in Pichia pastoris

    Depositor

    Sheng Yang

    Article

    Yang et al Yeast. 2014 Apr;31(4):115-25.

    Insert

    GFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available Since

    March 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMW#3

    Plasmid

    #13350

    Depositor

    Marian Walhout

    Article

    Deplancke et al Cell. 2006 Jun 16. 125(6):1193-205.

    Insert

    LacZ reporter
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Nov. 9, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pYES2/103Q

    Plasmid

    #1385

    Depositor

    Michael Sherman

    Article

    Meriin et al J Cell Biol 2002 Jun 10;157(6):997-1004.

    Insert

    huntingtin (103Q) (HTT Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Yeast

    Mutation

    Exon1 sequences containing the first 17 amino aci…

    Promoter

    Available Since

    Feb. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRY003

    Plasmid

    #81043

    Purpose

    HO endonuclease expression

    Depositor

    John McCusker

    Insert

    HO
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    endogenous

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pXN22-Dest

    Plasmid

    #105085

    Purpose

    Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

    Depositor

    Christopher Grefen

    Article

    Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

    Type

    Empty backbone

    Use

    Tags

    NubG-3xHA

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. PRS303-240xtetO

    Plasmid

    #44754

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    240x tetO array and CHRI homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBEVY-GU

    Plasmid

    #51229

    Purpose

    bi-directional expression vector for yeast, galactose inducible, URA3 selection

    Depositor

    Charles Miller

    Article

    Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1,10

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge